| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA4329767 | B | Inhibition of Cy5-preE-let-7g binding to recombinant human N-terminal His6-tagged Lin28a expressed in Escherichia coli Rosetta2 (DE3) pLysS measured after 30 to 60 mins by electrophoretic mobility shift assay | Homo sapiens | 2 | ALA4325922 | assay format | Scientific Literature | |
| 2. | ALA4329768 | B | Inhibition of Cy5-preE-let-7g binding to recombinant human N-terminal His6-tagged Lin28a expressed in Escherichia coli Rosetta2 (DE3) pLysS at 15 uM measured after 30 to 60 mins by electrophoretic mobility shift assay relative to control | Homo sapiens | 2 | ALA4325922 | assay format | Scientific Literature | |
| 3. | ALA4329769 | B | Inhibition of Cy5-preE-let-7a-1 binding to recombinant human N-terminal His6-tagged Lin28a expressed in Escherichia coli Rosetta2 (DE3) pLysS measured after 30 to 60 mins by electrophoretic mobility shift assay | Homo sapiens | 1 | ALA4325922 | assay format | Scientific Literature | |
| 4. | ALA4329770 | B | Inhibition of PreE-let-7a-1-BHQ2 binding to recombinant human N-terminal His6-tagged/Cy3-labelled Lin28a expressed in Escherichia coli Rosetta2 (DE3) pLysS measured after 30 to 60 mins by FRET assay | Homo sapiens | 1 | ALA4325922 | assay format | Scientific Literature | |
| 5. | ALA4329771 | B | Inhibition of pre-let-7g binding to recombinant human N-terminal His6-tagged Lin28a expressed in Escherichia coli Rosetta2 (DE3) pLysS assessed as increase in dicer-mediated pre-let-7g microRNA maturation at 5 to 10 uM measured after 40 mins by SYBR green2 dye based fluorescence assay | Homo sapiens | 1 | ALA4325922 | assay format | Scientific Literature | |
| 6. | ALA4329773 | B | Binding affinity to recombinant human N-terminal His6-tagged Lin28a cold shock domain expressed in Escherichia coli Rosetta2 (DE3) pLysS at 0.625 to 10 uM by surface plasmon resonance analysis | Homo sapiens | 1 | ALA4325922 | assay format | Scientific Literature | |
| 7. | ALA4329774 | B | Binding affinity to recombinant human N-terminal His6-tagged Lin28a zinc knuckle domain expressed in Escherichia coli Rosetta2 (DE3) pLysS at 0.625 to 10 uM by surface plasmon resonance analysis | Homo sapiens | 1 | ALA4325922 | assay format | Scientific Literature | |
| 8. | ALA4329776 | B | Inhibition of biotinylated-preE-let-7g binding to Lin28a in human JAR cells by pull down assay | Homo sapiens | 1 | ALA4325922 | cell-based format | Scientific Literature | |
| 9. | ALA4329777 | B | Binding affinity to recombinant human N-terminal His6-tagged Lin28a expressed in Escherichia coli Rosetta2 (DE3) pLysS by surface plasmon resonance analysis | Homo sapiens | 1 | ALA4325922 | assay format | Scientific Literature | |
| 10. | ALA4329792 | B | Inhibition of Lin28a in human PA1 cells assessed as increase in mature let-7d level at 10 uM after 20 hrs by TRIzol dye based qRT-PCR analysis | Homo sapiens | 1 | ALA4325922 | cell-based format | Scientific Literature | |
| 11. | ALA4329793 | B | Inhibition of Lin28a in human PA1 cells assessed as increase in mature let-7g level at 10 uM after 20 hrs by TRIzol dye based qRT-PCR analysis | Homo sapiens | 1 | ALA4325922 | cell-based format | Scientific Literature | |
| 12. | ALA4329794 | B | Inhibition of Lin28a in human PA1 cells assessed as increase in mature let-7i level at 10 uM after 20 hrs by TRIzol dye based qRT-PCR analysis | Homo sapiens | 1 | ALA4325922 | cell-based format | Scientific Literature | |
| 13. | ALA4329806 | B | Inhibition of Lin28a in human PA1 cells assessed as reduction in HMGA2 expression at 10 uM after 24 hrs by Western blot analysis | Homo sapiens | 1 | ALA4325922 | cell-based format | Scientific Literature | |
| 14. | ALA4329807 | B | Inhibition of Lin28a in human PA1 cells assessed as reduction in IMP1 expression at 10 uM after 24 hrs by Western blot analysis | Homo sapiens | 1 | ALA4325922 | cell-based format | Scientific Literature | |
| 15. | ALA4329808 | B | Inhibition of Lin28a in human PA1 cells assessed as reduction in Ras expression at 10 uM after 24 hrs by Western blot analysis | Homo sapiens | 1 | ALA4325922 | cell-based format | Scientific Literature | |
| 16. | ALA4329809 | B | Inhibition of Lin28a in human PA1 cells assessed as reduction in Lin28 expression at 10 uM after 24 hrs by Western blot analysis | Homo sapiens | 1 | ALA4325922 | cell-based format | Scientific Literature | |
| 17. | ALA4715226 | B | Inhibition of LIN28A cold shock domain (16 to 126 residues) (unknown origin) assessed as increase in thermal stability measured after 45 mins by differential scanning fluorimetry | Homo sapiens | 1 | ALA4706754 | single protein format | Scientific Literature | |
| 18. | ALA5233803 | B | Inhibition of LIN28A (unknown origin) by FRET assay | Homo sapiens | 4 | ALA5233220 | single protein format | Scientific Literature |
The page will load shortly, Thanks for your patience!