#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA3583124	Binding	Inhibition of Tel-fused FGFR4 (unknown origin) expressed in mouse BA/F3 cells after 48 hrs by luciferase reporter gene assay in absence of recombinant mouse IL3	Homo sapiens	4	ALA3580662	cell-based format	Scientific Literature
2.	ALA3630499	Binding	Inhibition of FGFR4 (unknown origin) at 10 uM after 120 mins P33 radiolabeled kinase activity assay	Homo sapiens	3	ALA3627608	single protein format	Scientific Literature
3.	ALA3706133	Binding	ADP-Glo Kinase Assay: In this assay, the inhibitory activity of a test substance against the tyrosine kinase activity of FGFR4 protein is measured.To each well of a flat bottom 96 well white plate (Sumitomo Bakelite Co., Ltd., MS-8496W), 10 ul FGFR4 protein (Carna Biosciences, Inc., 08-136) solution diluted to 1 ug/mL with an assay buffer (20 mM HEPES-NaOH, 0.01% Triton X-100, 2 mM DTT, 5 mM MgCl2 and 2 mM MnCl2), 10 uL of an assay buffer solution containing CSK-tide substrate (Ana Spec Inc., 63843) in a final concentration of 1000 nM and ATP (Promega Corporation, V9102) in a final concentration of 75 uM, and 5 ul of a test substance diluted with the assay buffer were added and the reaction was performed at room temperature for 2 hours (kinase reaction). For measuring kinase activity, ADP-Glo Kinase Assay (Promega Corporation, V9102) was used.		3	ALA3638780	single protein format	BindingDB Database
4.	ALA3707934	Binding	Inhibition Assay: Purified recombinant human FGFR4 protein was purchased from Carna Biosciences, Inc. When setting conditions for the measurement of the inhibitory effect of the compounds on FGFR4 kinase activity, a biotinylated peptide (biotin-EEPLYWSFPAKKK) was synthesized for use as a substrate by utilizing the amino acid sequence of FL-Peptide 22 (Caliper Life Sciences, Inc.) with biotin. The purified recombinant human FGFR4 protein used in the test was purchased from Carna Biosciences, Inc. In the measurement of the inhibitory effect of the compounds, first, a test compound was gradually diluted with dimethylsulfoxide (DMSO) to a concentration 20 times higher than the final concentration. Next, the purified human FGFR4 protein, substrate peptide (final concentration: 250 nM), magnesium chloride (final concentration: 5 mM), ATP (final concentration: 190 .mu.M), and the test compound DMSO solution (final concentration of DMSO: 5%) were added to a reaction buffer.		7	ALA3638928	single protein format	BindingDB Database
5.	ALA3751059	Binding	Inhibition of FGFR4 (unknown origin) at 1 uM	Homo sapiens	1	ALA3745631	single protein format	Scientific Literature
6.	ALA3750394	Binding	Inhibition of FGFR4 (unknown origin)	Homo sapiens	1	ALA3745712	single protein format	Scientific Literature
7.	ALA3748189	Binding	Inhibition of human FGFR4 using poly[Glu:Tyr] (4:1) as substrate	Homo sapiens	1	ALA3745707	single protein format	Scientific Literature
8.	ALA3762701	Binding	Inhibition of FGFR4 (unknown origin) at 100 nM	Homo sapiens	1	ALA3758169	single protein format	Scientific Literature
9.	ALA3779691	Binding	Inhibition of N-terminal His6-tagged recombinant human FGFR4 expressed in baculovirus infected insect Sf9 cells assessed as remaining activity at 1 uM relative to control	Homo sapiens	3	ALA3774357	cell-based format	Scientific Literature
10.	ALA3789988	Binding	Inhibition of FGFR4 (unknown origin) in presence of [gamma33P]ATP	Homo sapiens	6	ALA3785066	single protein format	Scientific Literature
11.	ALA3804522	Binding	Inhibition of recombinant FGFR4 (unknown origin) using (Glu,Tyr)4:1 as substrate incubated for 60 mins by ELISA	Homo sapiens	2	ALA3797059	single protein format	Scientific Literature
12.	ALA3816337	Binding	Inhibition of recombinant human His-tagged cytoplasmic FGFR4 (781 to 1338 residues) expressed in baculovirus expression system at 1 uM relative to control	Homo sapiens	1	ALA3813614	assay format	Scientific Literature
13.	ALA3816608	Binding	Inhibition of human FGFR4 at 1 uM using poly[Glu:Tyr] (4:1) as substrate	Homo sapiens	1	ALA3813650	single protein format	Scientific Literature
14.	ALA3820494	Binding	Inhibition of FGFR4 (unknown origin) incubated for 1 hr by spectrophotometric analysis	Homo sapiens	2	ALA3817824	single protein format	Scientific Literature
15.	ALA3820333	Binding	Inhibition of recombinant human N-terminal His6-tagged FGFR4 (442 to 755 residues) expressed in baculovirus infected Sf21 cells at 1 uM by radiometric assay relative to control	Homo sapiens	2	ALA3817791	cell-based format	Scientific Literature
16.	ALA3830174	Binding	Inhibition of recombinant human FGFR4 using poly (Glu, Tyr) 4:1 as substrate after 60 mins by ELISA	Homo sapiens	1	ALA3826910	single protein format	Scientific Literature
17.	ALA3830200	Binding	Inhibition of recombinant human FGFR4	Homo sapiens	1	ALA3826910	single protein format	Scientific Literature
18.	ALA839213	Binding	Selectivity for fibroblast growth factor receptor 4		1	ALA1146681	single protein format	Scientific Literature
19.	ALA1099660	Binding	Inhibition of FGFR4 at 10 uM		2	ALA1154277	single protein format	Scientific Literature
20.	ALA1108402	Binding	Inhibition of FGFR4 at 5 uM		1	ALA1154316	single protein format	Scientific Literature