# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4323086 | B | Binding affinity to recombinant human His-tagged SPIN1 (26 to 262 residues) expressed in Escherichia coli BL21 (DE3) assessed as melting temperature at 20 uM by Sypro orange dye based differential scanning fluorimetry | Homo sapiens | 6 | assay format | Scientific Literature | ||
2. | ALA4323093 | B | Binding affinity to recombinant human His-tagged SPIN1 (26 to 262 residues) expressed in Escherichia coli BL21 (DE3) assessed as dissociation constant by isothermal titration calorimetry | Homo sapiens | 2 | assay format | Scientific Literature | ||
3. | ALA4323097 | B | Displacement of trimethylated biotinylated histone H3 peptide from recombinant human His-tagged SPIN1 (26 to 262 residues) expressed in Escherichia coli BL21 (DE3) incubated for 30 mins followed by peptide addition and measured after 1 hr by Alphascreen method | Homo sapiens | 18 | assay format | Scientific Literature | ||
4. | ALA4323099 | B | Inhibition of C-terminal Halotag-histone 3.3 tagged SPIN1 (unknown origin) transfected in human U2OS cells cotransfected with nano-luciferase at 1 uM using NanoBRET Nano-Glo substrate as substrate incubated for 24 hrs by NanoBRET assay | Homo sapiens | 6 | cell-based format | Scientific Literature | ||
5. | ALA4323101 | B | Binding affinity to human His-tagged SPIN1 (21 to 262 residues) expressed in Escherichia coli BL21 (DE3) assessed as dissociation constant preincubated for 5 mins followed by compound addition by octet BLI assay | Homo sapiens | 6 | assay format | Scientific Literature | ||
6. | ALA4323102 | B | Binding affinity to recombinant human His-tagged SPIN1 (49 to 262 residues) expressed in Escherichia coli BL21 (DE3) assessed as dissociation constant by isothermal titration calorimetry | Homo sapiens | 7 | assay format | Scientific Literature | ||
7. | ALA4323124 | B | Binding affinity to recombinant human N-terminal His-tagged SPIN1 (50 to 262 residues) expressed in Escherichia coli BL21 (DE3) assessed as dissociation constant by isothermal titration calorimetry | Homo sapiens | 1 | assay format | Scientific Literature | ||
8. | ALA4323125 | B | Binding affinity to recombinant human His-tagged SPIN1 (49 to 262 residues) expressed in Escherichia coli BL21 (DE3) incubated for 30 mins by AlphaLISA assay | Homo sapiens | 1 | assay format | Scientific Literature | ||
9. | ALA4377283 | B | Inhibition of SPIN1 (unknown origin) assessed as dissociation constant by isothermal titration calorimetry | Homo sapiens | 1 | single protein format | Scientific Literature | ||
10. | ALA4377285 | B | Inhibition of H3(1-12)K4me3 binding to recombinant human C-terminal His6-tagged SPIN1 (49 to 262 residues) expressed in Escherichia coli BL21 (DE3) cells assessed as dissociation constant by FTSA assay | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
11. | ALA4377289 | B | Inhibition of FL-H3K4me3 binding to recombinant human C-terminal His6-tagged SPIN1 (49 to 262 residues) expressed in Escherichia coli BL21 (DE3) cells incubated for 30 mins by fluorescence polarization displacement assay | Homo sapiens | 5 | cell-based format | Scientific Literature | ||
12. | ALA4377290 | B | Inhibition of biotin-H3(1-23)K4me3 binding to recombinant human C-terminal His6-tagged SPIN1 (49 to 262 residues) expressed in Escherichia coli BL21 (DE3) cells incubated for 90 mins by AlphaLISA assay | Homo sapiens | 5 | cell-based format | Scientific Literature | ||
13. | ALA4377292 | B | Inhibition of recombinant human C-terminal His6-tagged SPIN1 (49 to 262 residues) expressed in Escherichia coli BL21 (DE3) cells assessed as dissociation constant by isothermal titration calorimetry | Homo sapiens | 3 | cell-based format | Scientific Literature | ||
14. | ALA4377295 | B | Inhibition of C-terminal Halo Tag-histone H3.3/N-terminal Nano Luciferase-fused full length SPIN1 (unknown origin) transfected in human U2OS cells assessed as reduction in SPIN1 and histone H3 interaction incubated for 24 hrs by NanoBRET assay | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
15. | ALA4377357 | B | Inhibition of recombinant human C-terminal His6-tagged SPIN1 (49 to 262 residues) expressed in Escherichia coli BL21 (DE3) cells assessed as binding to one tudor domain of protein by measuring molar ratio by isothermal titration calorimetry | Homo sapiens | 1 | cell-based format | Scientific Literature |