Inhibition of SARS-CoV-2 3CLpro preincubated for 30 mins followed by addition of (Dabcyl)KTSAVLQSGFRKM(Glu) peptide substrate and measured after 1.5 hrs by FRET assay
Inhibition of recombinant SARS CoV-2 main protease expressed in Escherichia coli using MCA-AVLQ SGFR-Lys (Dnp)-Lys-NH2 as substrate by FRET based assay
Inhibition of recombinant SARS-CoV-2 Main protease expressed in Escherichia coli BL21 (DE3) preincubated for 30 mins followed by addition of DABCYL-Lys-Thr-Ser-Ala-Val-Leu-Gln-Ser-Gly-Phe-Arg-Lys-Met-Glu-EDANS substrate and measured after 30 mins by microplate reader method
Inhibition of SARS-CoV-2 Main protease expressed in Escherichia coli BL21 (DE3) preincubated for 30 mins followed by addition of Dabcyl-KTSAVLQ/SGFRKME(Edans) substrate and measured after 1 hr by FRET assay
Inhibition of SARS-CoV-2 3CL (3264 to 3569 residues) preincubated for 30 mins followed by addition of Dabcyl-KTSAVLQSGFRKME(Edans) substrate and measured after 1 hr by flourescence based plate reader assay
Competitive inhibition of SARS CoV-2 main protease using varying concentrations of DABCYL-KTSAVLQ1SGFRKM-E(EDANS)-NH2 as substrate by Dixon plot analysis
Inhibition of SARS-CoV-2 main protease at 50 uM using Dabcy1-KTSAVLQSGFRKM-E-Edans-NH2 as substrate incubated for 10 mins followed by substrate addition by FRET assay relative to control
Inhibition of SARS-CoV-2 3CLpro using TVLQ-AMC probe as substrate at 10 uM preincubated with enzyme for 5 mins followed by substrate addition and measured after 3 hrs for every 15 mins for 1 hr by FRET assay
Inhibition of SARS-CoV-2 3CLpro using TVLQ-AMC probe as substrate at 50 uM preincubated with enzyme for 5 mins followed by substrate addition and measured after 3 hrs for every 15 mins for 1 hr by FRET assay
Inhibition of SARS-CoV-2 RdRp transfected in HEK293T cells cotransfected with CoV-Gluc assessed as decrease in plus and minus strand-Gluc RNA levels incubated for 24 hrs by qRT-PCR analysis