# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4415964 | Binding | Protac activity against VHL/BCR-ABL in human K562 cells assessed as induction of BCR-ABL degradation at 10 uM by Western blot analysis | Homo sapiens | 14 | ALA4414590 | cell-based format | Scientific Literature | |
2. | ALA4415965 | Binding | Protac activity against VHL/BCR-ABL in human K562 cells assessed as induction of BCR-ABL degradation by Western blot analysis | Homo sapiens | 7 | ALA4414590 | cell-based format | Scientific Literature | |
3. | ALA4415967 | Binding | Protac activity against VHL/BCR-ABL in human K562 cells assessed as induction of BCR-ABL degradation at 1 to 100 nM by Western blot analysis | Homo sapiens | 1 | ALA4414590 | cell-based format | Scientific Literature | |
4. | ALA4415968 | Binding | Protac activity against VHL/BCR-ABL in human K562 cells assessed as induction of BCR-ABL degradation at 100 nM by Western blot analysis | Homo sapiens | 1 | ALA4414590 | cell-based format | Scientific Literature | |
5. | ALA4415977 | Binding | Protac activity against VHL/BCR-ABL in human K562 cells assessed as induction of BCR-ABL degradation at 100 nM incubated up to 24 hrs by Western blot analysis | Homo sapiens | 1 | ALA4414590 | cell-based format | Scientific Literature | |
6. | ALA4415979 | Binding | Protac activity against VHL/BCR-ABL in human K562 cells assessed as induction of BCR-ABL degradation pre-treated with 4 uM VHL1 for 2 hrs followed by compound addition at 100 nM and further incubated for 8 hrs by Western blot analysis | Homo sapiens | 1 | ALA4414590 | cell-based format | Scientific Literature | |
7. | ALA4415980 | Binding | Protac activity against VHL/BCR-ABL in human K562 cells assessed as induction of BCR-ABL degradation pre-treated with 100 nM dasatinib for 2 hrs followed by compound addition at 100 nM and further incubated for 8 hrs by Western blot analysis | Homo sapiens | 1 | ALA4414590 | cell-based format | Scientific Literature | |
8. | ALA4415981 | Binding | Protac activity against VHL/BCR-ABL in human K562 cells assessed as induction of BCR-ABL degradation pre-treated with 2.5 uM proteasome inhibitor MG132 for 2 hrs followed by compound addition at 100 nM and further incubated for 8 hrs by Western blot analysis | Homo sapiens | 1 | ALA4414590 | cell-based format | Scientific Literature | |
9. | ALA4415982 | Binding | Induction of ternary complex formation between VHL and BCR-ABL (unknown origin) using recombinant ABL kinase domain and recombinant VHL/EloB/EloC by SEC-MALS analysis | Homo sapiens | 3 | ALA4414590 | protein format | Scientific Literature | |
10. | ALA4415983 | Binding | Protac activity against VHL/BCR-ABL in human K562 cell lysates assessed as induction of pull down of endogenous BCR-ABL at 10 to 100 nM in presence of excess immobilized GST-VBC bait by GST-pull down assay | Homo sapiens | 1 | ALA4414590 | subcellular format | Scientific Literature | |
11. | ALA4415984 | Binding | Protac activity against VHL/BCR-ABL in human K562 cell lysates assessed as induction of pull down of endogenous ABL at 10 to 100 nM in presence of excess immobilized GST-VBC bait by GST-pull down assay | Homo sapiens | 1 | ALA4414590 | subcellular format | Scientific Literature | |
12. | ALA4415985 | Binding | Induction of ternary complex formation between VHL and BCR-ABL (unknown origin) expressed in HEK293 cells expressing N-terminal BCR-ABL LgBit fusion protein and N-terminal SmBit-VHL fusion protein at 1 uM incubated for 1 hr by NanoBit technology based luciferase complementation assay | Homo sapiens | 2 | ALA4414590 | cell-based format | Scientific Literature | |
13. | ALA4415986 | Binding | Induction of ternary complex formation between VHL and BCR-ABL (unknown origin) expressed in HEK293 cells expressing N-terminal BCR-ABL LgBit fusion protein and N-terminal SmBit-VHL fusion protein pre-treated with 10 uM dasatinib for 2 hrs followed by compound addition at 1 uM and further incubated for 1 hr by NanoBit technology based luciferase complementation assay | Homo sapiens | 1 | ALA4414590 | cell-based format | Scientific Literature | |
14. | ALA4415990 | Binding | Induction of ternary complex formation between VHL and BCR-ABL (unknown origin) expressed in HEK293 cells expressing N-terminal BCR-ABL LgBit fusion protein and N-terminal SmBit-VHL fusion protein incubated for 1 hr by NanoBit technology based luciferase complementation assay | Homo sapiens | 2 | ALA4414590 | cell-based format | Scientific Literature | |
15. | ALA4415991 | Binding | Protac activity against VHL/BCR-ABL in human K562 cells assessed as reduction in ABL2 protein levels at 100 nM incubated for 16 hrs by multiplexed tandem mass spectrometry | Homo sapiens | 1 | ALA4414590 | cell-based format | Scientific Literature | |
16. | ALA4415994 | Binding | Protac activity against VHL/BCR-ABL in human K562 cells assessed as reduction in BCR-ABL protein levels at 1 to 100 nM incubated for 16 hrs by by Western blot analysis | Homo sapiens | 1 | ALA4414590 | cell-based format | Scientific Literature | |
17. | ALA4416011 | Binding | Protac activity against VHL/BCR-ABL (unknown origin) expressed in human U937 cells assessed as induction of BCR-ABL degradation incubated for 16 hrs by Western blot analysis | Homo sapiens | 1 | ALA4414590 | cell-based format | Scientific Literature | |
18. | ALA4416013 | Binding | Protac activity against VHL/BCR-ABL V299L mutant (unknown origin) expressed in human U937 cells assessed as induction of BCR-ABL degradation incubated for 16 hrs by Western blot analysis | Homo sapiens | 1 | ALA4414590 | cell-based format | Scientific Literature | |
19. | ALA4416020 | Binding | Protac activity against VHL/BCR-ABL in human K562 cells assessed as induction of BCR-ABL degradation at 20 times IC50 incubated for 12 hrs followed by drug wash-out and incubation in drug-free medium up to 72 hrs by Western blot analysis | Homo sapiens | 3 | ALA4414590 | cell-based format | Scientific Literature | |
20. | ALA4416021 | Binding | Protac activity against VHL/BCR-ABL in human K562 cells assessed as reduction in BCR-ABL phosphorylation at 20 times IC50 incubated for 12 hrs followed by drug wash-out and incubation in drug-free medium up to 72 hrs by Western blot analysis | Homo sapiens | 3 | ALA4414590 | cell-based format | Scientific Literature |