# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4376261 | B | Inhibition of Pseudomonas aeruginosa tRNA (guanine(37)-N1)-methyltransferase (Leu5 to Asp25 residues) expressed in Escherichia coli BL21 (DE3) Rosetta T1R cells assessed as reduction in S-adenosyl-L-homocysteine production preincubated for 15 mins followed by 1.5 uM of tRNALeu(CAG) substrate and SAM addition and measured after 45 mins by MTase-Glo assay | Pseudomonas aeruginosa | 26 | ALA4373727 | cell-based format | Scientific Literature | |
2. | ALA4376262 | B | Inhibition of Pseudomonas aeruginosa tRNA (guanine(37)-N1)-methyltransferase (Leu5 to Asp25 residues) expressed in Escherichia coli BL21 Rosetta (DE3) assessed as reduction in S-adenosyl-L-homocysteine production preincubated for 15 mins followed by 4.5 uM of tRNALeu(CAG) substrate and SAM addition and measured after 45 mins by MTase-Glo assay | Pseudomonas aeruginosa | 2 | ALA4373727 | assay format | Scientific Literature | |
3. | ALA4376263 | B | Inhibition of Pseudomonas aeruginosa tRNA (guanine(37)-N1)-methyltransferase (Leu5 to Asp25 residues) expressed in Escherichia coli BL21 Rosetta (DE3) assessed as reduction in S-adenosyl-L-homocysteine production preincubated for 15 mins followed by 15 uM of tRNALeu(CAG) substrate and SAM addition and measured after 45 mins by MTase-Glo assay | Pseudomonas aeruginosa | 2 | ALA4373727 | assay format | Scientific Literature | |
4. | ALA4376264 | B | Binding affinity to Pseudomonas aeruginosa tRNA (guanine(37)-N1)-methyltransferase (Leu5 to Asp25 residues) expressed in Escherichia coli BL21 (DE3) Rosetta T1R cells by surface plasmon resonance assay | Pseudomonas aeruginosa | 3 | ALA4373727 | cell-based format | Scientific Literature | |
5. | ALA4376267 | B | Binding affinity to Pseudomonas aeruginosa tRNA (guanine(37)-N1)-methyltransferase (Leu5 to Asp25 residues) expressed in Escherichia coli BL21 (DE3) Rosetta T1R cells assessed as association rate constant by surface plasmon resonance assay | Pseudomonas aeruginosa | 3 | ALA4373727 | cell-based format | Scientific Literature | |
6. | ALA4376270 | B | Binding affinity to Pseudomonas aeruginosa tRNA (guanine(37)-N1)-methyltransferase (Leu5 to Asp25 residues) expressed in Escherichia coli BL21 (DE3) Rosetta T1R cells assessed as dissociation rate constant by surface plasmon resonance assay | Pseudomonas aeruginosa | 3 | ALA4373727 | cell-based format | Scientific Literature | |
7. | ALA4376273 | B | Binding affinity to Pseudomonas aeruginosa tRNA (guanine(37)-N1)-methyltransferase (Leu5 to Asp25 residues) expressed in Escherichia coli BL21 (DE3) Rosetta T1R cells assessed as thermal stabilization of protein by measuring change in melting temperature at 0.1 mM by SYPRO orange based thermal shift assay | Pseudomonas aeruginosa | 11 | ALA4373727 | cell-based format | Scientific Literature | |
8. | ALA4376274 | B | Binding affinity to Pseudomonas aeruginosa tRNA (guanine(37)-N1)-methyltransferase (Leu5 to Asp25 residues) expressed in Escherichia coli BL21 (DE3) Rosetta T1R cells assessed as thermal stabilization of protein by measuring change in melting temperature at 2 mM by SYPRO orange based thermal shift assay | Pseudomonas aeruginosa | 1 | ALA4373727 | cell-based format | Scientific Literature | |
9. | ALA4376275 | B | Binding affinity to Pseudomonas aeruginosa tRNA (guanine(37)-N1)-methyltransferase (Leu5 to Asp25 residues) expressed in Escherichia coli BL21 (DE3) Rosetta T1R cells assessed as thermal stabilization of protein by measuring melting temperature at 0.1 mM by SYPRO orange based thermal shift assay (Rvb = 50 degreeC) | Pseudomonas aeruginosa | 2 | ALA4373727 | cell-based format | Scientific Literature | |
10. | ALA4376276 | B | Binding affinity to Pseudomonas aeruginosa tRNA (guanine(37)-N1)-methyltransferase (Leu5 to Asp25 residues) expressed in Escherichia coli BL21 (DE3) Rosetta T1R cells assessed as thermal stabilization of protein by measuring melting temperature at 2 mM by SYPRO orange based thermal shift assay (Rvb = 50 degreeC) | Pseudomonas aeruginosa | 1 | ALA4373727 | cell-based format | Scientific Literature | |
11. | ALA4376309 | B | Inhibition of Pseudomonas aeruginosa tRNA (guanine(37)-N1)-methyltransferase (Leu5 to Asp25 residues) expressed in Escherichia coli BL21 (DE3) Rosetta T1R cells assessed as reduction in S-adenosyl-L-homocysteine production preincubated for 30 mins followed by 1.5 to 15 uM of tRNALeu(CAG) substrate and SAM addition and measured after 60 mins by MTase-Glo assay | Pseudomonas aeruginosa | 1 | ALA4373727 | cell-based format | Scientific Literature | |
12. | ALA4376310 | B | Inhibition of Pseudomonas aeruginosa tRNA (guanine(37)-N1)-methyltransferase (Leu5 to Asp25 residues) expressed in Escherichia coli BL21 (DE3) Rosetta T1R cells assessed as reduction in S-adenosyl-L-homocysteine production up to 50 uM preincubated for 15 mins followed by 1.5 uM of tRNALeu(CAG) substrate and SAM addition and measured after 45 mins by MTase-Glo assay | Pseudomonas aeruginosa | 8 | ALA4373727 | cell-based format | Scientific Literature |