# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4383291 | Binding | Inhibition of Enterococcus faecalis Ddl | Enterococcus faecalis | 1 | ALA4382202 | single protein format | Scientific Literature | |
2. | ALA4383292 | Binding | Inhibition of Enterococcus faecalis JH2-2 C-terminal 6His-tagged DdlB expressed in Escherichia coli LMG194 preincubated for 30 mins followed by substrate addition and measured after 20 mins by Biomol Green reagent assay | Enterococcus faecalis | 22 | ALA4382202 | assay format | Scientific Literature | |
3. | ALA4383293 | Binding | Inhibition of Enterococcus faecalis JH2-2 C-terminal 6His-tagged DdlB expressed in Escherichia coli LMG194 at 10 uM preincubated for 30 mins followed by substrate addition and measured after 20 mins by Biomol Green reagent assay relative to control | Enterococcus faecalis | 24 | ALA4382202 | assay format | Scientific Literature | |
4. | ALA4383294 | Binding | Inhibition of Enterococcus faecalis JH2-2 C-terminal 6His-tagged DdlB expressed in Escherichia coli LMG194 assessed as residual activity at 10 uM preincubated for 30 mins followed by substrate addition and measured after 20 mins by Biomol Green reagent assay relative to control | Enterococcus faecalis | 18 | ALA4382202 | assay format | Scientific Literature | |
5. | ALA4383295 | Binding | Inhibition of Enterococcus faecalis JH2-2 C-terminal 6His-tagged DdlB expressed in Escherichia coli LMG194 at IC50 after 10 mins by Biomol Green reagent assay relative to control | Enterococcus faecalis | 1 | ALA4382202 | assay format | Scientific Literature | |
6. | ALA4383296 | Binding | Inhibition of Enterococcus faecalis JH2-2 C-terminal 6His-tagged DdlB expressed in Escherichia coli LMG194 at IC50 after 90 mins by Biomol Green reagent assay relative to control | Enterococcus faecalis | 1 | ALA4382202 | assay format | Scientific Literature | |
7. | ALA4383356 | Binding | Inhibition of Enterococcus faecalis JH2-2 C-terminal 6His-tagged DdlB expressed in Escherichia coli LMG194 preincubated for 30 mins followed by substrate addition and measured after 20 mins in presence of 0.01% Triton X-100 by Biomol Green reagent assay | Enterococcus faecalis | 1 | ALA4382202 | assay format | Scientific Literature | |
8. | ALA4383357 | Binding | Reversible inhibition of Enterococcus faecalis JH2-2 C-terminal 6His-tagged DdlB expressed in Escherichia coli LMG194 at 3 uM preincubated for 30 mins followed by 10 times dilution followed by 20 mins incubation with substrate by Biomol Green reagent assay | Enterococcus faecalis | 1 | ALA4382202 | assay format | Scientific Literature | |
9. | ALA4383358 | Binding | Mixed non-competitive type inhibition of Enterococcus faecalis JH2-2 C-terminal 6His-tagged DdlB expressed in Escherichia coli LMG194 at 1 to 10 uM preincubated for 30 mins followed increasing substrate and fixed ATP addition and measured after 20 mins by Biomol Green reagent assay | Enterococcus faecalis | 1 | ALA4382202 | assay format | Scientific Literature | |
10. | ALA4383359 | Binding | Inhibition of Enterococcus faecalis JH2-2 C-terminal 6His-tagged DdlB expressed in Escherichia coli LMG194 assessed as Km1 of D-Ala using 500 uM ATP by Biomol Green reagent assay | Enterococcus faecalis | 1 | ALA4382202 | assay format | Scientific Literature | |
11. | ALA4383360 | Binding | Inhibition of Enterococcus faecalis JH2-2 C-terminal 6His-tagged DdlB expressed in Escherichia coli LMG194 assessed as Km2 of D-Ala using 500 uM ATP by Biomol Green reagent assay | Enterococcus faecalis | 1 | ALA4382202 | assay format | Scientific Literature | |
12. | ALA4383361 | Binding | Inhibition of Enterococcus faecalis JH2-2 C-terminal 6His-tagged DdlB expressed in Escherichia coli LMG194 assessed as Km of ATP using 50 mM D-Ala substrate by Biomol Green reagent assay | Enterococcus faecalis | 1 | ALA4382202 | assay format | Scientific Literature | |
13. | ALA4383366 | Binding | Inhibition of Enterococcus faecalis JH2-2 C-terminal 6His-tagged DdlB expressed in Escherichia coli LMG194 assessed as Vmax of D-Ala at 1 uM by Biomol Green reagent assay (Rvb = 61.95 +/- 1.11 microM/min) | Enterococcus faecalis | 1 | ALA4382202 | assay format | Scientific Literature | |
14. | ALA4383367 | Binding | Inhibition of Enterococcus faecalis JH2-2 C-terminal 6His-tagged DdlB expressed in Escherichia coli LMG194 assessed as Vmax of D-Ala at 10 uM by Biomol Green reagent assay (Rvb = 61.95 +/- 1.11 microM/min) | Enterococcus faecalis | 1 | ALA4382202 | assay format | Scientific Literature | |
15. | ALA4383368 | Binding | Inhibition of Enterococcus faecalis JH2-2 C-terminal 6His-tagged DdlB expressed in Escherichia coli LMG194 assessed as Km of D-Ala at 1 uM by Biomol Green reagent assay (Rvb = 5.62 mM) | Enterococcus faecalis | 1 | ALA4382202 | assay format | Scientific Literature | |
16. | ALA4383369 | Binding | Inhibition of Enterococcus faecalis JH2-2 C-terminal 6His-tagged DdlB expressed in Escherichia coli LMG194 assessed as Km of D-Ala at 10 uM by Biomol Green reagent assay (Rvb = 5.62 mM) | Enterococcus faecalis | 1 | ALA4382202 | assay format | Scientific Literature | |
17. | ALA4383370 | Binding | Inhibition of Enterococcus faecalis JH2-2 C-terminal 6His-tagged DdlB expressed in Escherichia coli LMG194 assessed as Vmax/Km of D-Ala at 1 uM by Biomol Green reagent assay (Rvb = 11.02 /min) | Enterococcus faecalis | 1 | ALA4382202 | assay format | Scientific Literature | |
18. | ALA4383371 | Binding | Inhibition of Enterococcus faecalis JH2-2 C-terminal 6His-tagged DdlB expressed in Escherichia coli LMG194 assessed as Vmax/Km of D-Ala at 10 uM by Biomol Green reagent assay (Rvb = 11.02 /min) | Enterococcus faecalis | 1 | ALA4382202 | assay format | Scientific Literature | |
19. | ALA4383372 | Binding | Inhibition of Enterococcus faecalis JH2-2 C-terminal 6His-tagged DdlB expressed in Escherichia coli LMG194 assessed as Vmax of ATP at 1 uM by Biomol Green reagent assay (Rvb = 89.05 +/- 5.41 microM/min) | Enterococcus faecalis | 1 | ALA4382202 | assay format | Scientific Literature | |
20. | ALA4383373 | Binding | Inhibition of Enterococcus faecalis JH2-2 C-terminal 6His-tagged DdlB expressed in Escherichia coli LMG194 assessed as Vmax of ATP at 10 uM by Biomol Green reagent assay (Rvb = 89.05 +/- 5.41 microM/min) | Enterococcus faecalis | 1 | ALA4382202 | assay format | Scientific Literature |