#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA4334288	B	Non-competitive inhibition of C-terminal His6-tagged linked Zika virus NS2B-NS3 protease domain expressed in Escherichia coli Bl21(DE3) using Bz-Nle-Lys-Lys-Arg-AMC as substrate after 30 mins by Cornish-Bowden plot analysis	Zika virus	6		assay format	Scientific Literature
2.	ALA4334289	B	Inhibition of C-terminal His6-tagged linked Zika virus NS2B-NS3 protease domain expressed in Escherichia coli Bl21(DE3) using Bz-Nle-Lys-Lys-Arg-AMC as substrate after 30 mins by fluorophotometric method	Zika virus	5		assay format	Scientific Literature
3.	ALA4334290	B	Binding affinity to C-terminal His6-tagged linked Zika virus NS2B-NS3 protease domain by SPR analysis	Zika virus	6		single protein format	Scientific Literature
4.	ALA4334291	B	Inhibition of C-terminal His6-tagged unlinked Zika virus NS2B-NS3 protease domain expressed in Escherichia coli Bl21(DE3) using Bz-Nle-Lys-Lys-Arg-AMC as substrate after 30 mins by fluorophotometric method	Zika virus	6		assay format	Scientific Literature
5.	ALA4334296	B	Inhibition of C-terminal His6-tagged unlinked Zika virus NS2B-NS3 protease domain expressed in Escherichia coli Bl21(DE3) at 200 to 400 nM using Bz-Nle-Lys-Lys-Arg-AMC as substrate after 30 mins in presence of cn-716 by fluorophotometric method	Zika virus	1		assay format	Scientific Literature
6.	ALA4334299	B	Competitive inhibition of C-terminal His6-tagged linked Zika virus NS2B-NS3 protease domain expressed in Escherichia coli Bl21(DE3) using Bz-Nle-Lys-Lys-Arg-AMC as substrate after 30 mins by Cornish-Bowden plot analysis	Zika virus	6		assay format	Scientific Literature
7.	ALA4338581	B	Inhibition of Zika virus NS2B/NS3 protease	Zika virus	1		single protein format	Scientific Literature
8.	ALA4338583	B	Inhibition of N-terminal hexa-histidine tagged Zika virus NS2B/NS3 protease expressed in Escherichia coli BL21-Gold (DE3) using fluorogenic AMC-substituted peptide boc-GRR-7-amino-4-methylcoumarin measured every 30 secs for 10 mins by fluorometric assay	Zika virus	27		assay format	Scientific Literature
9.	ALA4338585	B	Inhibition of N-terminal hexa-histidine tagged Zika virus NS2B/NS3 protease expressed in Escherichia coli BL21-Gold (DE3) at 20 uM using fluorogenic AMC-substituted peptide boc-GRR-7-amino-4-methylcoumarin measured every 30 secs for 10 mins by fluorometric assay relative to control	Zika virus	8		assay format	Scientific Literature
10.	ALA4338587	B	Binding affinity to N-terminal hexa-histidine tagged Zika virus NS2B/NS3 protease expressed in Escherichia coli BL21-Gold (DE3) by microscale thermophoresis method	Zika virus	2		assay format	Scientific Literature
11.	ALA4338588	B	Non-competitive inhibition of N-terminal hexa-histidine tagged Zika virus NS2B/NS3 protease expressed in Escherichia coli BL21-Gold (DE3) using 50 to 200 uM fluorogenic AMC-substituted peptide boc-GRR-7-amino-4-methylcoumarin measured every 30 secs for 10 mins by fluorometric assay based Dixon plot analysis	Zika virus	1		assay format	Scientific Literature
12.	ALA4355616	B	Inhibition of N-terminal His6-SUMO tagged Zika virus Puerto Rico NS2B-NS3 linked protease expressed in Escherichia coli BL21 Codon Plus (DE3) cells preincubated for 10 mins followed by pyr-TGKR-AMC addition and measured every 5 secs for 2 mins by fluorometric enzyme assay	Zika virus	1		cell-based format	Scientific Literature
13.	ALA4355617	B	Inhibition of N-terminal His6-SUMO tagged Zika virus Puerto Rico NS2B-NS3 unlinked protease expressed in Escherichia coli BL21 Codon Plus (DE3) cells preincubated for 10 mins followed by pyr-TGKR-AMC addition and measured every 5 secs for 2 mins by fluorometric enzyme assay	Zika virus	1		cell-based format	Scientific Literature
14.	ALA4355618	B	Inhibition of N-terminal His6-SUMO tagged Zika virus Puerto Rico NS2B-NS3 unlinked protease expressed in Escherichia coli BL21 Codon Plus (DE3) cells preincubated for 90 mins followed by pyr-TGKR-AMC addition and measured every 5 secs for 2 mins by fluorometric enzyme assay	Zika virus	1		cell-based format	Scientific Literature
15.	ALA4355619	B	Inhibition of Zika virus IbH_30656 NS2B (45 to 96 residues)-NS3 (1 to 177 residues) protease expressed in Escherichia coli BL21/Rosetta (DE3) cells preincubated for 60 mins followed by Bz-nKRR-AMC addition and measured at 60 secs intervals over 10 mins by fluorescence method	Zika virus	1		cell-based format	Scientific Literature
16.	ALA4355620	B	Inhibition of Zika virus unlinked NS2B-NS3 protease preincubated for 5 mins followed by benzoyl-nKRR-AMC addition and measured every 60 secs for 12 mins by fluorescence method	Zika virus	3		single protein format	Scientific Literature
17.	ALA4355964	B	Inhibition of N-terminal His6-tagged Zika virus NS2B (49 to 95 residues) - NS3 (1 to 170 residues) protease domain expressed in Escherichia coli BL21-Gold (DE3) cells preincubated for 10 mins followed by Bz-Nle-Lys-Lys-Arg-AMC substrate addition	Zika virus	1		cell-based format	Scientific Literature
18.	ALA4356007	B	Inhibition of N-terminal His6-tagged Zika virus NS2B (45 to 96 residues) - NS3 (1 to 177 residues) protease domain expressed in Escherichia coli BL21-Gold (DE3) cells or Escherichia coli BL21 Rosetta (DE3) preincubated for 30 mins followed by Bz-nKRR-AM substrate addition measured over 10 mins at 60 secs interval by fluorescence method	Zika virus	2		cell-based format	Scientific Literature
19.	ALA4356008	B	Inhibition of wild type Zika virus NS2B-NS3 protease preincubated for 30 mins followed by Pyr-RTKR-AMC substrate addition	Zika virus	1		single protein format	Scientific Literature
20.	ALA4356009	B	Inhibition of Zika virus NS2B (1421 to 1469 residues) - NS3 (1503 to 1688 residues) expressed in Escherichia coli BL21(DE3) cells using Dabcyl-KTSAVLQSGFRKME-Edans as substrate after 11 mins by FRET assay	Zika virus	7		cell-based format	Scientific Literature