#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA4311618	B	Inhibition of calf thymus DNA topoisomerase 1 assessed as reduction in enzyme-mediated relaxation of supercoiled pBR322 plasmid DNA at 10 uM after 30 mins by agarose gel electrophoresis	Bos taurus	6		single protein format	Scientific Literature
2.	ALA4311619	B	Inhibition of calf thymus DNA topoisomerase 1 assessed as reduction in enzyme-mediated relaxation of supercoiled pBR322 plasmid DNA at 10 uM after 30 mins by ethidium bromide staining-based agarose gel electrophoresis	Bos taurus	6		single protein format	Scientific Literature
3.	ALA4347111	B	Inhibition of calf thymus topoisomerase1-mediated relaxation of supercoiled pBR322 DNA at 5 uM measured after 30 mins by ethidium bromide staining based agarose gel electrophoresis	Bos taurus	1		single protein format	Scientific Literature
4.	ALA4385195	B	Inhibition of calf thymus topoisomerase-1-mediated supercoiled pBR322 DNA relaxation at 50 uM after 30 mins by agarose gel electrophoresis assay relative to control	Bos taurus	5		single protein format	Scientific Literature
5.	ALA4385196	B	Inhibition of calf thymus topoisomerase-1 assessed as reduction in cleavage complex formation by measuring decrease in nicked form DNA using supercoiled pBR322 DNA as substrate after 30 mins by agarose gel electrophoresis assay	Bos taurus	1		single protein format	Scientific Literature
6.	ALA4385197	B	Inhibition of calf thymus topoisomerase-1 assessed as reduction in cleavage complex formation by measuring nicked form DNA level using supercoiled pBR322 DNA as substrate after 30 mins by agarose gel electrophoresis assay (Rvb = 47%)	Bos taurus	3		single protein format	Scientific Literature
7.	ALA4385198	B	Inhibition of supercoiled pBR322 DNA binding to calf thymus topoisomerase-1 after 0.5 hrs by agarose gel electrophoresis assay	Bos taurus	2		single protein format	Scientific Literature
8.	ALA4385199	B	Inhibition of calf thymus topoisomerase-1-mediated supercoiled pBR322 DNA unwinding at 25 uM preincubated with DNA for 10 mins followed by enzyme addition and measured after 30 mins by agarose gel electrophoresis assay	Bos taurus	3		single protein format	Scientific Literature
9.	ALA4385200	B	Inhibition of calf thymus topoisomerase-1-mediated relaxed pBR322 DNA unwinding at 25 uM preincubated with DNA for 10 mins followed by enzyme addition and measured after 30 mins by agarose gel electrophoresis assay	Bos taurus	3		single protein format	Scientific Literature
10.	ALA4385857	B	Inhibition of calf thymus DNA topoisomerase 1 using supercoiled pBR322 plasmid DNA as substrate at 20 uM after 30 mins by agarose gel electrophoresis method relative to control	Bos taurus	2		single protein format	Scientific Literature
11.	ALA4411457	B	Inhibition of calf thymus DNA topoisomerase 1 assessed as decrease in relaxation of supercoiled pBR322 DNA after 30 mins by Genview nucleic acid staining based agarose gel electrophoresis	Bos taurus	1		single protein format	Scientific Literature
12.	ALA4613618	B	Inhibition of calf thymus gland topoisomerase 1 mediated DNA relaxation	Bos taurus	2		single protein format	Scientific Literature
13.	ALA4623530	B	Inhibition of calf thymus Top1 assessed as reduction in supercoiled pBR322 DNA relaxation at 100 uM measured after 30 mins by agarose gel electrophoresis relative to camptothecin	Bos taurus	27		single protein format	Scientific Literature
14.	ALA4623538	B	Inhibition of calf thymus Top1-mediated supercoiled pBR322 DNA cleavage assessed as nicked DNA content at 25 uM measured after 30 mins by ethidium bromide staining agarose gel electrophoresis (Rvb = 15%)	Bos taurus	4		single protein format	Scientific Literature
15.	ALA4623539	B	Inhibition of calf thymus Top1-mediated supercoiled pBR322 DNA cleavage assessed as nicked DNA content at 50 uM measured after 30 mins by ethidium bromide staining agarose gel electrophoresis (Rvb = 15%)	Bos taurus	4		single protein format	Scientific Literature
16.	ALA4623540	B	Inhibition of calf thymus Top1 assessed as reduction in supercoiled pBR322 DNA relaxation measured after 30 mins by agarose gel electrophoresis	Bos taurus	3		single protein format	Scientific Literature
17.	ALA4623541	B	Inhibition of calf thymus Top1 assessed as reduction in Top1-supercoiled pBR322 DNA covalent cleavage formation at 25 to 50 uM measured after 30 mins by electrophoretic mobility shift assay	Bos taurus	3		single protein format	Scientific Literature