#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA4380225	ADME	Substrate activity at Staphylococcus saprophyticus nitroreductase expressed in Escherichia coli BL21 (DE3) assessed as retention time by HPLC analysis	Staphylococcus saprophyticus	36	ALA4376859	assay format	Scientific Literature
2.	ALA4380229	ADME	Substrate activity at Staphylococcus saprophyticus nitroreductase NtrB assessed as Kcat using NADPH as cofactor in Tris-Cl buffer at pH 7.5 incubated for 30 mins by HPLC analysis	Staphylococcus saprophyticus	2	ALA4376859	single protein format	Scientific Literature
3.	ALA4380231	ADME	Substrate activity at Staphylococcus saprophyticus nitroreductase NtrB assessed as Kcat using NADPH as cofactor in Tris-Cl buffer at pH 7.5 by HPLC analysis	Staphylococcus saprophyticus	2	ALA4376859	single protein format	Scientific Literature
4.	ALA4380232	ADME	Substrate activity at Staphylococcus saprophyticus nitroreductase NtrB assessed as Kcat using NADH as cofactor in Tris-Cl buffer at pH 7.5 by HPLC analysis	Staphylococcus saprophyticus	7	ALA4376859	single protein format	Scientific Literature
5.	ALA4380235	ADME	Substrate activity at Staphylococcus saprophyticus nitroreductase NtrB assessed as Km using NADPH as cofactor in Tris-Cl buffer at pH 7.5 incubated for 30 mins by HPLC analysis	Staphylococcus saprophyticus	2	ALA4376859	single protein format	Scientific Literature
6.	ALA4380238	ADME	Substrate activity at Staphylococcus saprophyticus nitroreductase NtrB assessed as Km using NADPH as cofactor in Tris-Cl buffer at pH 7.5 by HPLC analysis	Staphylococcus saprophyticus	2	ALA4376859	single protein format	Scientific Literature
7.	ALA4380239	ADME	Substrate activity at Staphylococcus saprophyticus nitroreductase NtrB assessed as Km using NADH as cofactor in Tris-Cl buffer at pH 7.5 by HPLC analysis	Staphylococcus saprophyticus	7	ALA4376859	single protein format	Scientific Literature
8.	ALA4380243	ADME	Substrate activity at Staphylococcus saprophyticus nitroreductase NtrB assessed as ratio of Kcat/Km using NADPH as cofactor in Tris-Cl buffer at pH 7.5 incubated for 30 mins by HPLC analysis	Staphylococcus saprophyticus	2	ALA4376859	single protein format	Scientific Literature
9.	ALA4380245	ADME	Substrate activity at Staphylococcus saprophyticus nitroreductase NtrB assessed as Kcat/Km using NADPH as cofactor in Tris-Cl buffer at pH 7.5 by HPLC analysis	Staphylococcus saprophyticus	2	ALA4376859	single protein format	Scientific Literature
10.	ALA4380246	ADME	Substrate activity at Staphylococcus saprophyticus nitroreductase NtrB assessed as Kcat/Km using NADH as cofactor in Tris-Cl buffer at pH 7.5 by HPLC analysis	Staphylococcus saprophyticus	7	ALA4376859	single protein format	Scientific Literature
11.	ALA4380250	ADME	Substrate activity at Staphylococcus saprophyticus nitroreductase NtrB assessed as ratio of Kcat/Km using NADPH as cofactor in Tris-Cl buffer at pH 7.5 incubated for 30 mins by HPLC analysis relative to CB1954/NfsB	Staphylococcus saprophyticus	2	ALA4376859	single protein format	Scientific Literature
12.	ALA4380252	ADME	Substrate activity at Staphylococcus saprophyticus nitroreductase NtrB assessed as Kcat/Km using NADPH as cofactor in Tris-Cl buffer at pH 7.5 by HPLC analysis relative to CB1954/NfsB	Staphylococcus saprophyticus	2	ALA4376859	single protein format	Scientific Literature
13.	ALA4380253	ADME	Substrate activity at Staphylococcus saprophyticus nitroreductase NtrB assessed as Kcat/Km using NADH as cofactor in Tris-Cl buffer at pH 7.5 by HPLC analysis relative to CB1954/NfsB	Staphylococcus saprophyticus	6	ALA4376859	single protein format	Scientific Literature
14.	ALA4380254	ADME	Substrate activity at Staphylococcus saprophyticus nitroreductase NtrB assessed as enzyme specific activity using NADH as cofactor in Tris-Cl buffer at pH 7.5 by HPLC analysis	Staphylococcus saprophyticus	11	ALA4376859	single protein format	Scientific Literature
15.	ALA4380255	ADME	Prodrug activation assessed as Ssap-NtrB-mediated metabolite formation measured after 15 mins by LC-MS/MS analysis	Staphylococcus saprophyticus	1	ALA4376859	single protein format	Scientific Literature
16.	ALA4380258	Binding	Binding affinity to Staphylococcus saprophyticus nitroreductase NtrB	Staphylococcus saprophyticus	4	ALA4376859	single protein format	Scientific Literature
17.	ALA4380265	ADME	Substrate activity at Staphylococcus saprophyticus nitroreductase expressed in Escherichia coli BL21 (DE3) assessed as enzyme mediated reduction at 50 to 150 uM measured up 20 hrs by HPLC analysis in presence of NADPH	Staphylococcus saprophyticus	36	ALA4376859	assay format	Scientific Literature
18.	ALA4394190	ADME	Prodrug conversion assessed as Staphylococcus saprophyticus nitroreductase mediated P2 metabolite formation in presence of FDH cofactor regeneration system at 100 uM by HPLC analysis	Staphylococcus saprophyticus	1	ALA4393661	single protein format	Scientific Literature
19.	ALA4394191	ADME	Substrate activity at Staphylococcus saprophyticus nitroreductase expressed in Escherichia coli assessed as retention time in the presence of NADPH at 100 uM by HPLC analysis (0 to 5 mins at 20 % ACN, 15 to 22 min at 80% ACN, 27-32 AT 20% ACN)	Staphylococcus saprophyticus	16	ALA4393661	assay format	Scientific Literature
20.	ALA4394192	ADME	Substrate activity at Staphylococcus saprophyticus nitroreductase expressed in Escherichia coli assessed as half life in the presence of NADPH at 100 uM by HPLC analysis (0 to 5 mins at 20 % ACN, 15 to 22 min at 80% ACN, 27-32 AT 20% ACN)	Staphylococcus saprophyticus	10	ALA4393661	assay format	Scientific Literature