# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4308949 | B | Inhibition of recombinant mTORC2 (unknown origin) expressed in Escherichia coli after 15 mins in presence of gamma-[32]P-ATP by high-throughput screening assay | Homo sapiens | 1 | ALA4308824 | assay format | Scientific Literature | |
2. | ALA4308964 | B | Inhibition of mTORC2 (unknown origin) | Homo sapiens | 5 | ALA4308824 | protein complex format | Scientific Literature | |
3. | ALA4308966 | B | Inhibition of N-terminally FLAG-tagged mTORC2 (unknown origin) expressed in human HeLa cells using S6K1 or Akt1 as substrate after 20 mins by immunoblotting assay | Homo sapiens | 2 | ALA4308824 | cell-based format | Scientific Literature | |
4. | ALA4308973 | B | Inhibition of mTORC2 in HEK293 cells using GST-tagged S6K1 or Akt1 as substrate after 30 mins by immunoblotting assay | Homo sapiens | 1 | ALA4308824 | cell-based format | Scientific Literature | |
5. | ALA4322495 | B | Inhibition of mTORC2 in human A2058 cells assessed as reduction in PKB phosphorylation at S473 residues incubated for 1 hr by Western blot analysis | Homo sapiens | 29 | ALA4321805 | cell-based format | Scientific Literature | |
6. | ALA4352823 | B | Inhibition of mTORC2 in human RD cells assessed as reduction in Akt phosphorylation at Ser473 site at 20 uM incubated for 2 hrs in presence of insulin by Western blot analysis | Homo sapiens | 19 | ALA4350972 | cell-based format | Scientific Literature | |
7. | ALA4370820 | B | Inhibition of mTORC2 signaling in human HEK293 cells assessed as reduction in leucine-induced AKT phosphorylation at 200 uM preincubated for 50 mins followed by leucine-stimulation and measured after 10 mins by Western blot analysis | Homo sapiens | 10 | ALA4368933 | cell-based format | Scientific Literature | |
8. | ALA4410951 | B | Inhibition of mTORC2 in human A431 cells assessed as AKT phosphorylation at S473 residue incubated for 3 hrs by HTRF assay | Homo sapiens | 19 | ALA4406914 | cell-based format | Scientific Literature | |
9. | ALA4432844 | B | Inhibition of mTORC2 in HEK293T/17 cells assessed as reduction in Akt phosphorylation at Ser-473 residue after 2 hrs by sandwich immunoassay | Homo sapiens | 1 | ALA4431376 | cell-based format | Scientific Literature | |
10. | ALA4668257 | B | Inhibition of mTORC2 in human A2058 cells assessed as reduction in PKB phosphorylation at S473 residue incubated for 1 hr by Western blot analysis | Homo sapiens | 4 | ALA4665671 | cell-based format | Scientific Literature | |
11. | ALA5127283 | B | Inhibition of mTORC2 (unknown origin) | Homo sapiens | 1 | ALA5126514 | protein complex format | Scientific Literature | |
12. | ALA5216191 | B | Inhibition of mTORC2 (unknown origin) | Homo sapiens | 19 | ALA5214923 | protein complex format | Scientific Literature | |
13. | ALA5216193 | B | Inhibition of mTORC2 in human PC-3 cells assessed as measuring phosphorylated Akt level incubated for 24 hrs by AlphaLISA assay | Homo sapiens | 5 | ALA5214923 | cell-based format | Scientific Literature | |
14. | ALA5216212 | B | Inhibition of mTORC2 (unknown origin) assessed as measuring phosphorylated AKT at 0.5 uM relative to control | Homo sapiens | 3 | ALA5214923 | protein complex format | Scientific Literature | |
15. | ALA5216216 | B | Inhibition of mTORC2 in human A-431 cells assessed as phosphorylated AKT level incubated for 3 hrs by HTRF assay | Homo sapiens | 12 | ALA5214923 | cell-based format | Scientific Literature | |
16. | ALA5216223 | B | Inhibition of mTORC2 in human MCF7 cells assessed as measuring phosphorylated AKT incubated for 2 hrs by AlphaLISA assay | Homo sapiens | 15 | ALA5214923 | cell-based format | Scientific Literature | |
17. | ALA5216227 | B | Inhibition of mTORC2 in human MDA-MB-468 cells assessed as measuring phosphorylated AKT incubated for 2 to 4 hrs by AlphaLISA assay | Homo sapiens | 8 | ALA5214923 | cell-based format | Scientific Literature | |
18. | ALA5225765 | B | Inhibition of mTORC2 in human LNCaP C4-2B cells assessed as inhibition of phosphorylation of AKT at Ser473 at 5 uM incubated for 2 hrs by Western blot analysis | Homo sapiens | 1 | ALA5223168 | cell-based format | Scientific Literature | |
19. | ALA5231734 | B | Inhibition of mTORC2 in human HEK293 cells assessed as decrease in phosphorylated Akt level incubated for 30 mins by immunoblot assay | Homo sapiens | 1 | ALA5230127 | cell-based format | Scientific Literature | |
20. | ALA5231762 | B | Inhibition of mTORC2 in human LN-229 cells assessed as decrease in phosphorylation of AKT at Ser473 level incubated for 24 hrs by immunoblot assay | Homo sapiens | 1 | ALA5230127 | cell-based format | Scientific Literature |