# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4418529 | Functional | Antiproliferative activity against human SK-MEL-147 cells assessed as inhibition of LPA-stimulated cell proliferation at 0.3 mmol/L and measured on day 8 by WST1 based assay | Homo sapiens | 1 | ALA4418480 | cell-based format | Patent Bioactivity Data | |
2. | ALA4623431 | Functional | Cytotoxicity against human SK-MEL-147 cells assessed as reduction in cell viability at EC50 concentration preincubated for 1 hr in presence of GSH measured after 24 hrs by MTT assay | Homo sapiens | 2 | ALA4622851 | cell-based format | Scientific Literature | |
3. | ALA4623432 | Functional | Cytotoxicity against human SK-MEL-147 cells assessed as reduction in cell viability at 50 and 100 uM after 24 hrs by MTT assay | Homo sapiens | 23 | ALA4622851 | cell-based format | Scientific Literature | |
4. | ALA4623434 | Functional | Cytotoxicity against human SK-MEL-147 cells assessed as reduction in cell viability after 24 hrs by MTT assay | Homo sapiens | 23 | ALA4622851 | cell-based format | Scientific Literature | |
5. | ALA4623436 | Functional | Induction of apoptosis in human SK-MEL-147 cells assessed as early apoptotic cells at 20 uM after 24 hrs by Annexin V-FITC/propidium iodide staining based flow cytometry | Homo sapiens | 2 | ALA4622851 | cell-based format | Scientific Literature | |
6. | ALA4623437 | Functional | Induction of apoptosis in human SK-MEL-147 cells assessed as late apoptotic cells at 20 uM after 24 hrs by Annexin V-FITC/propidium iodide staining based flow cytometry | Homo sapiens | 2 | ALA4622851 | cell-based format | Scientific Literature | |
7. | ALA4623438 | Functional | Induction of apoptosis in human SK-MEL-147 cells assessed as necrotic cells at 20 uM after 24 hrs by Annexin V-FITC/propidium iodide staining based flow cytometry | Homo sapiens | 2 | ALA4622851 | cell-based format | Scientific Literature | |
8. | ALA4623439 | Functional | Induction of apoptosis in human SK-MEL-147 cells assessed as mitochondrial membrane potential dissipation after 24 hrs by TMRM staining based fluorescence microscopic method | Homo sapiens | 2 | ALA4622851 | cell-based format | Scientific Literature | |
9. | ALA4623440 | Functional | Induction of apoptosis in human SK-MEL-147 cells assessed as increase in reactive oxygen species generation by CM-H2DCFDA staining based flow cytometric analysis | Homo sapiens | 2 | ALA4622851 | cell-based format | Scientific Literature | |
10. | ALA4623441 | Functional | Induction of apoptosis in human SK-MEL-147 cells assessed mitochondrial permeabilization after 24 hrs by mitotracker green staining based microscopic analysis | Homo sapiens | 2 | ALA4622851 | cell-based format | Scientific Literature | |
11. | ALA4623442 | Functional | Cytotoxicity against human SK-MEL-147 cells assessed as reduction in cell viability at EC50 concentration preincubated for 1 hr in presence of DTT measured after 24 hrs by MTT assay | Homo sapiens | 2 | ALA4622851 | cell-based format | Scientific Literature | |
12. | ALA4623443 | Functional | Cytotoxicity against human SK-MEL-147 cells assessed as reduction in cell viability at EC50 concentration preincubated for 1 hr in presence of N-acetyl cysteine measured after 24 hrs by MTT assay | Homo sapiens | 2 | ALA4622851 | cell-based format | Scientific Literature | |
13. | ALA4623446 | Functional | Cytotoxicity against human SK-MEL-147 cells assessed as reduction in cell viability at EC50 concentration preincubated for 1 hr in presence of Boc-D-FMK measured after 24 hrs by MTT assay | Homo sapiens | 2 | ALA4622851 | cell-based format | Scientific Literature | |
14. | ALA4623447 | Functional | Cytotoxicity against human SK-MEL-147 cells assessed as reduction in cell viability at EC50 concentration preincubated for 1 hr in presence of E-64 measured after 24 hrs by MTT assay | Homo sapiens | 2 | ALA4622851 | cell-based format | Scientific Literature | |
15. | ALA4623456 | Functional | Induction of apoptosis in human SK-MEL-147 cells assessed as increase in BAX expression at 20 uM after 24 hrs by western blotting analysis | Homo sapiens | 2 | ALA4622851 | cell-based format | Scientific Literature | |
16. | ALA4623457 | Functional | Induction of apoptosis in human SK-MEL-147 cells assessed as effect on BCL-2 protein expression at 20 uM after 24 hrs by western blotting analysis | Homo sapiens | 2 | ALA4622851 | cell-based format | Scientific Literature | |
17. | ALA4623458 | Functional | Cytotoxicity against human SK-MEL-147 cells assessed as reduction in cell viability in presence of BCL2 inhibitor venetoclax | Homo sapiens | 2 | ALA4622851 | cell-based format | Scientific Literature | |
18. | ALA5151563 | Functional | Cytotoxicity against human SK-MEL-147 cells assessed as cell growth inhibition measured after 24 hrs by WST-1 assay | Homo sapiens | 2 | ALA5149994 | cell-based format | Scientific Literature |