#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA3706299	Binding	In Vitro Inhibition Assay: This work was performed at the MDS Pharma Services, Pharmacology Laboratories, Taiwan. The assay was an in vitro evaluation of the ability of an extract or a pure compound to inhibit the steroid 5alpha -reductase enzyme from metabolizing testosterone into dihydrotestosterone. This is an enzyme-immunoassay (EIA) for quantitative determination of testosterone in human serum or plasma. The significance of this type of inhibition is that it can lead to eradication of benign prostatic hyperplasia (BPH). Two distinct isozymes are found in mice, rats, monkeys and humans: type 1 and II. Each of these isozymes is differentially expressed in tissues and developmental stages. In human, type 1 steroid 5alpha -reductase is predominant in the sebaceous glands of most regions of skin, including scalp and liver and is responsible for approximately one third of circulating DHT. Inhibitors of steroid 5alpha -reductase may be of benefit in the treatment of androgenetic alopecia.	Homo sapiens	2	ALA3638782	tissue-based format	BindingDB Database
2.	ALA3737756	Binding	Inhibition of human prostate type 2 5alpha-reductase assessed as transformation of testosterone to dihydrotestosterone	Homo sapiens	13	ALA3734650	tissue-based format	Scientific Literature
3.	ALA3756855	Binding	Inhibition of human 5alpha-2 reductase expressed in HEK293 cells assessed as suppression of conversion of [3]androstenedione at 10 uM incubated for 30 mins by radioactivity-based HPLC analysis	Homo sapiens	6	ALA3751829	cell-based format	Scientific Literature
4.	ALA3756856	Binding	Inhibition of human 5alpha-2 reductase expressed in HEK293 cells assessed as suppression of conversion of [3]androstenedione at 2 uM incubated for 30 mins by radioactivity-based HPLC analysis	Homo sapiens	6	ALA3751829	cell-based format	Scientific Literature
5.	ALA3756857	Binding	Inhibition of human 5alpha-2 reductase expressed in HEK293 cells assessed as suppression of conversion of [3]androstenedione incubated for 30 mins by radioactivity-based HPLC analysis	Homo sapiens	7	ALA3751829	cell-based format	Scientific Literature
6.	ALA884527	Binding	Compound was tested for the inhibition of type 2 5-alpha-reductase of human prostates		6	ALA1134121	tissue-based format	Scientific Literature
7.	ALA873205	Binding	Inhibition of recombinant human 5-alpha reductase-2 at a concentration of 5 microL after pre-incubation for 10 minutes		23	ALA1126579	single protein format	Scientific Literature
8.	ALA841571	Binding	Inhibitory concentration against human recombinant steroid 5-alpha-reductase type 2 in stably transfected chinese hamster ovary 1827 cells using [3H]testosterone		1	ALA1148350	assay format	Scientific Literature
9.	ALA828727	Binding	Inhibitory concentration against human steroid 5-alpha-reductase 2 receptor expressed in CHO 1829 cells	Homo sapiens	21	ALA1145142	cell-based format	Scientific Literature
10.	ALA827275	Functional	Inhibition of [1-beta-2beta-3H]- -testosterone binding to human steroid 5-alpha-reductase type 2 of BPH tissue at 10 uM	Homo sapiens	2	ALA1143608	assay format	Scientific Literature
11.	ALA832273	Binding	Inhibitory concentration against human steroid 5-alpha-reductase 2 receptor expressed in CHO 1829 cells; la= low activity	Homo sapiens	9	ALA1145142	cell-based format	Scientific Literature
12.	ALA835269	Functional	Inhibition of [1-beta-2beta-3H]- -testosterone binding to human steroid 5-alpha-reductase type 2 of BPH tissue at 10 uM	Homo sapiens	3	ALA1143608	assay format	Scientific Literature
13.	ALA834907	Functional	Inhibition of [1-beta-2beta-3H]- -testosterone binding to human steroid 5-alpha-reductase type 2 of BPH tissue at 10 uM; - = not determined	Homo sapiens	7	ALA1143608	assay format	Scientific Literature
14.	ALA863950	Binding	Displacement of [3H]-[1-beta,2beta]-testosterone from 5-alpha reductase2 in human prostate homogenate at 10 uM	Homo sapiens	4	ALA1147727	assay format	Scientific Literature
15.	ALA859578	Binding	Displacement of [3H]-[1-beta,2beta]-testosterone from 5alpha reductase2 in human prostate homogenate	Homo sapiens	11	ALA1147727	assay format	Scientific Literature
16.	ALA896327	Binding	Inhibition of steroid 5-alpha reductase type 2 expressed in HEK 293 cells at 10 uM		1	ALA1148877	cell-based format	Scientific Literature
17.	ALA896329	Binding	Inhibition of steroid 5-alpha reductase type 2 expressed in HEK 293 cells		1	ALA1148877	cell-based format	Scientific Literature
18.	ALA1106729	Binding	Inhibition of human 5alpha reductase 2 expressed in HEK293 cells assessed as inhibition of conversion of [14C]4-androstene-3,17-dione to [14C]5R-androstane-3,17-dione at 0.3 uM after 3 hrs	Homo sapiens	3	ALA1154295	cell-based format	Scientific Literature
19.	ALA809999	Binding	Binding affinity for human 5-alpha reductase 2 isozyme	Homo sapiens	13	ALA1129998	single protein format	Scientific Literature
20.	ALA810000	Binding	Inhibition of type 2 steroid-5-alpha-reductase		26	ALA1127399	single protein format	Scientific Literature