#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA750485	B	Binding affinity for nAChR with [3H]-nicotine in rat alpha4 beta2 membranes		2		cell membrane format	Scientific Literature
2.	ALA751868	B	Blockade of Nicotinic acetylcholine receptor alpha4-beta2		1		single protein format	Scientific Literature
3.	ALA751869	B	Blockage of Nicotinic acetylcholine receptor alpha4-beta2 noncompetitively in CA1 stratum radiatum interneurons		1		single protein format	Scientific Literature
4.	ALA751870	B	Blockage of Nicotinic acetylcholine receptor alpha4-beta2 noncompetitively in acute hippocampal slices		1		tissue-based format	Scientific Literature
5.	ALA751871	B	Blockage of Nicotinic acetylcholine receptor alpha4-beta2 noncompetitively in cultured hippocampal neurons		1		tissue-based format	Scientific Literature
6.	ALA3706132	B	Radioligand Binding: The binding of [3H]MLA was measured using a modification of the methods of Davies et al., Neuropharmacol. 38: 679 (1999). [3H]MLA (Specific Activity=25-35 Ci/mmol) was obtained from Tocris. The binding of [3H]MLA was determined using a 2 h incubation at 21 C. Incubations were conducted in 48-well micro-titre plates and contained about 200 μg of protein per well in a final incubation volume of 300 uL. The incubation buffer was PBS and the final concentration of [3H]MLA was 5 nM. The binding reaction was terminated by filtration of the protein containing bound ligand onto glass fiber filters (GF/B, Brandel) using a Brandel Tissue Harvester at room temperature. Filters were soaked in de-ionized water containing 0.33% polyethyleneimine to reduce non-specific binding. Each filter was washed with PBS (3x1 mL) at room temperature. Non-specific binding was determined by inclusion of 50 uM non-radioactive MLA in selected wells.	Rattus norvegicus	79		single protein format	BindingDB Database