| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA5106878 | B | Binding affinity to PARK7 in human HEK293T cells at 1 uM incubated for 1 hr by fluorescence scanning based analysis | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 2. | ALA5106879 | B | Binding affinity to N-terminal GST-tagged human recombinant PARK7 expressed in Escherichia coli BL21(DE3)Rosetta2 cells at 5 uM measured after 1 hr by coomassie staining based analysis | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 3. | ALA5106880 | B | Binding affinity to N-terminal GST-tagged human recombinant PARK7 expressed in Escherichia coli BL21(DE3)Rosetta2 cells at 1 uM measured after 1 hr by coomassie staining based analysis | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
| 4. | ALA5106881 | B | Binding affinity to N-terminal GST-tagged human recombinant PARK7 expressed in Escherichia coli BL21(DE3)Rosetta2 cells at 5 uM measured after 1 hr by fluorescence scanning analysis | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 5. | ALA5106882 | B | Binding affinity to N-terminal GST-tagged human recombinant PARK7 expressed in Escherichia coli BL21(DE3)Rosetta2 cells at 1 uM measured after 1 hr by fluorescence scanning analysis | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
| 6. | ALA5106887 | B | Inhibition of N-terminal GST-tagged human recombinant PARK7 expressed in Escherichia coli BL21(DE3)Rosetta2 cells using DiFMUAc as substrate preincubated with enzyme for 1 hr followed by substrate addition and measured for 1 hr by DiFMUAc assay based fluorescence analysis | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 7. | ALA5106888 | B | Inhibition of N-terminal GST-tagged human recombinant PARK7 expressed in Escherichia coli BL21(DE3)Rosetta2 cells using SRK59 as fluorescent probe at 0.5 uM preincubated for 1 hr followed by SRK59 addition for 30 mins by Gel-based competition assay | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 8. | ALA5106890 | B | Inhibition of N-terminal GST-tagged human recombinant PARK7 expressed in Escherichia coli BL21(DE3)Rosetta2 cells using SRK59 as fluorescent probe at 1 to 2 uM preincubated for 1 hr followed by SRK59 addition for 30 mins by Gel-based competition assay | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 9. | ALA5106891 | B | Binding affinity to PARK7 in human HEK293T cells at 0.5 uM incubated for 1 hr by fluorescence scanning based analysis | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 10. | ALA5106894 | B | Binding affinity to N-terminal GST-tagged human recombinant PARK7 expressed in Escherichia coli BL21(DE3)Rosetta2 cells at 2 uM measured after 1 hr by coomassie staining based analysis | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 11. | ALA5106895 | B | Binding affinity to N-terminal GST-tagged human recombinant PARK7 expressed in Escherichia coli BL21(DE3)Rosetta2 cells at 2 uM measured after 1 hr by fluorescence scanning analysis | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 12. | ALA5106896 | B | Binding affinity to PARK7 in human HEK293T cells at 0.5 to 1 uM incubated for 1 hr by western blot analysis | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 13. | ALA5106897 | B | Inhibition of N-terminal GST-tagged human recombinant PARK7 expressed in Escherichia coli BL21(DE3)Rosetta2 cells using SRK59 as fluorescent probe at 2 uM preincubated for 1 hr followed by SRK59 addition for 30 mins by Gel-based competition assay | Homo sapiens | 7 | cell-based format | Scientific Literature | ||
| 14. | ALA5106898 | B | Binding affinity to PARK7 in UCHL1 knockdown human HEK293T cells at 1 uM incubated for 1 hr by fluorescence scanning based analysis | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 15. | ALA5106899 | B | Inhibition of PARK7 in human HEK293T cells assessed as reduction in JQ-92 labelled PARK7 band intensity incubated for 1 hr followed by incubation with JQ-92 for 30 mins by fluorescence scanning based analysis | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 16. | ALA5106901 | B | Binding affinity to N-terminal GST-tagged human recombinant PARK7 expressed in Escherichia coli BL21(DE3)Rosetta2 cells assessed as ratio of Kinact/Ki by fluorescence polarization assay | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 17. | ALA5106902 | B | Inhibition of N-terminal GST-tagged human recombinant PARK7 expressed in Escherichia coli BL21(DE3)Rosetta2 cells assessed as reduction in JQ-107-labelled PARK7 band intensity incubated for 1 hr followed by incubation with JQ-107 and measured for 2 hrs by fluorescence polarization assay | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 18. | ALA5106903 | B | Inhibition of N-terminal GST-tagged human recombinant PARK7 expressed in Escherichia coli BL21(DE3)Rosetta2 cells using DiFMUAc as substrate at 12.5 to 100 uM preincubated with enzyme for 1 hr followed by substrate addition and measured for 1 hr by DiFMUAc assay based fluorescence analysis | Homo sapiens | 3 | cell-based format | Scientific Literature | ||
| 19. | ALA5106904 | B | Inhibition of PARK7 in human HEK293T cells assessed as reduction in JQ-92 labelled PARK7 band intensity at 1 uM incubated for 1 hr followed by incubation with JQ-92 for 30 mins by fluorescence scanning based analysis | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 20. | ALA5106905 | B | Inhibition of PARK7 in human HEK293T cells assessed as reduction in JQ-92 labelled PARK7 band intensity at 0.1 to 50 uM incubated for 1 hr followed by incubation with JQ-92 for 30 mins by fluorescence scanning based analysis | Homo sapiens | 3 | cell-based format | Scientific Literature |
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