| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA5107654 | B | Inhibition of human PHPT1 expressed in Escherichia coli BL21 (DE3) using pNPP as substrate incubated for 1 hr by multimode microplate reader analysis | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 2. | ALA5107655 | B | Covalent inhibition of human PHPT1 expressed in Escherichia coli BL21 (DE3) using pNPP as substrate at 10 equiv preincubated for 60 mins by multimode microplate reader analysis | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 3. | ALA5107656 | B | Covalent inhibition of human PHPT1 expressed in Escherichia coli BL21 (DE3) assessed as inhibition of dephosphorylation of histidine-phosphorylated BSA at 10 equiv preincubated for 1 hr followed by substrate addition and measured for 150 secs by Western blot analysis | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 4. | ALA5107657 | B | Covalent inhibition of human PHPT1 expressed in Escherichia coli BL21 (DE3) assessed as increase in protein mass at 10 equiv incubated for 1 hr by RP-HPLC based ESI-MS analysis | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 5. | ALA5107663 | B | Inhibition of human PHPT1 expressed in Escherichia coli BL21 (DE3) using pNPP as substrate at 25 uM incubated for 1 hr by multimode microplate reader analysis | Homo sapiens | 4 | assay format | Scientific Literature | ||
| 6. | ALA5107664 | B | Inhibition of human PHPT1 expressed in Escherichia coli BL21 (DE3) using pNPP as substrate assessed as Kinact by reciprocal analysis | Homo sapiens | 2 | assay format | Scientific Literature | ||
| 7. | ALA5107665 | B | Inhibition of human PHPT1 expressed in Escherichia coli BL21 (DE3) using pNPP as substrate assessed as inhibition constant by reciprocal analysis | Homo sapiens | 2 | assay format | Scientific Literature | ||
| 8. | ALA5107666 | B | Inhibition of human PHPT1 expressed in Escherichia coli BL21 (DE3) using pNPP as substrate assessed as Kinact/Ki by reciprocal analysis | Homo sapiens | 2 | assay format | Scientific Literature | ||
| 9. | ALA5125515 | B | Mixed inhibition of PHPT1 (unknown origin) assessed as change in Km and Vmax at above 150 uM using DifMUP as substrate by Michaelis-Menten based analysis | Homo sapiens | 2 | single protein format | Scientific Literature | ||
| 10. | ALA5125516 | B | Inhibition of PHPT1 (unknown origin) using DiFMUP as fluorogenic substrate incubated for 30 mins followed by substrate addition and measured every 60 seconds for 30 mins by fluorogenic assay | Homo sapiens | 4 | single protein format | Scientific Literature | ||
| 11. | ALA5125517 | B | Competitive inhibition of PHPT1 (unknown origin) assessed as change in Km and Vmax at below 150 uM using DifMUP as substrate by Michaelis-Menten based analysis | Homo sapiens | 2 | single protein format | Scientific Literature | ||
| 12. | ALA5125518 | B | Time dependent inhibition of PHPT1 (unknown origin) at 20 uM using DiFMUP as fluorogenic substrate preincubated for 15 to 120 mins followed by substrate addition and measured for 30 mins by fluorogenic assay | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 13. | ALA5125519 | B | Time dependent inhibition of PHPT1 (unknown origin) at 10 uM using DiFMUP as fluorogenic substrate preincubated for 15 to 120 mins followed by substrate addition and measured for 30 mins by fluorogenic assay | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 14. | ALA5125520 | B | Time dependent inhibition of PHPT1 (unknown origin) at 150 uM using DiFMUP as fluorogenic substrate preincubated for 15 to 120 mins followed by substrate addition and measured for 30 mins by fluorogenic assay | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 15. | ALA5125521 | B | Time dependent inhibition of PHPT1 (unknown origin) at 100 uM using DiFMUP as fluorogenic substrate preincubated for 15 to 120 mins followed by substrate addition and measured for 30 mins by fluorogenic assay | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 16. | ALA5125522 | B | Inhibition of PHPT1 (unknown origin) assessed as inhibition constant measured up to 100 uM incubated up to 30 mins using DiFMUP as fluorogenic substrate by fluorogenic assay | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 17. | ALA5125523 | B | Inhibition of PHPT1 (unknown origin) assessed as inactivation constant measured up to 100 uM incubated up to 30 mins using DiFMUP as fluorogenic substrate by fluorogenic assay | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 18. | ALA5125524 | B | Reversible inhibition of PHPT1 (unknown origin) at 1 mM using DiFMUP as fluorogenic substrate preincubated for 30 mins followed by substrate addition by dialysis method | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 19. | ALA5125525 | B | Reversible inhibition of PHPT1 (unknown origin) at 100 uM using DiFMUP as fluorogenic substrate preincubated for 30 mins followed by substrate addition by dialysis method | Homo sapiens | 1 | single protein format | Scientific Literature |
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