| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA5126002 | B | Inhibition of Mycobacterium tuberculosis PafA expressed in Escherichia coli BL-21(DE3) assessed as inhibition of pup-ylation using Mtb PanB substrate at 20 uM by SDS-PAGE analysis | Mycobacterium tuberculosis | 5 | assay format | Scientific Literature | ||
| 2. | ALA5126004 | B | Inhibition of Mycobacterium tuberculosis PafA expressed in Escherichia coli BL-21(DE3) assessed as inhibition of pup-ylation using Mtb PanB substrate by SDS-PAGE analysis | Mycobacterium tuberculosis | 2 | assay format | Scientific Literature | ||
| 3. | ALA5126005 | B | Binding affinity to Mycobacterium tuberculosis PafA expressed in Escherichia coli BL-21(DE3) assessed as increase in thermal stability at 40 uM incubated for 15 mins at 25 degC followed by incubated at 52 to 72 degC for 5 mins by CESTA analysis | Mycobacterium tuberculosis | 1 | assay format | Scientific Literature | ||
| 4. | ALA5126007 | B | Inhibition of Mycobacterium tuberculosis PafA expressed in Escherichia coli BL-21(DE3) assessed as inhibition of pup-ylation using Mtb InhA substrate at 20 uM by SDS-PAGE analysis | Mycobacterium tuberculosis | 1 | assay format | Scientific Literature | ||
| 5. | ALA5126008 | B | Inhibition of Mycobacterium tuberculosis PafA expressed in Escherichia coli BL-21(DE3) assessed as inhibition of pup-ylation using Mtb KasA substrate at 20 uM by SDS-PAGE analysis | Mycobacterium tuberculosis | 1 | assay format | Scientific Literature | ||
| 6. | ALA5126009 | B | Inhibition of Mycobacterium tuberculosis PafA expressed in Escherichia coli BL-21(DE3) assessed as inhibition of pup-ylation using Mtb PknB substrate at 20 uM by SDS-PAGE analysis | Mycobacterium tuberculosis | 1 | assay format | Scientific Literature | ||
| 7. | ALA5126010 | B | Inhibition of Mycobacterium tuberculosis PafA expressed in Escherichia coli BL-21(DE3) assessed as inhibition of pup-ylation using Mtb DHFR substrate at 20 uM by SDS-PAGE analysis | Mycobacterium tuberculosis | 1 | assay format | Scientific Literature | ||
| 8. | ALA5126011 | B | Binding affinity to Mycobacterium tuberculosis PafA expressed in Escherichia coli BL-21(DE3) assessed as increase in thermal stability by measuring change in melting temperature in at 50 uM by CESTA analysis | Mycobacterium tuberculosis | 1 | assay format | Scientific Literature | ||
| 9. | ALA5126012 | B | Activation of Mycobacterium tuberculosis PafA expressed in Escherichia coli BL-21(DE3) assessed as pup-ylation using Mtb PanB substrate at 20 uM by SDS-PAGE analysis | Mycobacterium tuberculosis | 1 | assay format | Scientific Literature | ||
| 10. | ALA5126013 | B | Activation of Mycobacterium tuberculosis PafA expressed in Escherichia coli BL-21(DE3) assessed as pup-ylation using Mtb InhA substrate at 20 uM by SDS-PAGE analysis | Mycobacterium tuberculosis | 1 | assay format | Scientific Literature | ||
| 11. | ALA5126014 | B | Activation of Mycobacterium tuberculosis PafA expressed in Escherichia coli BL-21(DE3) assessed as pup-ylation using Mtb PknB substrate at 20 uM by SDS-PAGE analysis | Mycobacterium tuberculosis | 1 | assay format | Scientific Literature | ||
| 12. | ALA5126015 | B | Activation of Mycobacterium tuberculosis PafA expressed in Escherichia coli BL-21(DE3) assessed as pup-ylation using Mtb KasA substrate at 20 uM by SDS-PAGE analysis | Mycobacterium tuberculosis | 1 | assay format | Scientific Literature | ||
| 13. | ALA5126016 | B | Activation of Mycobacterium tuberculosis PafA expressed in Escherichia coli BL-21(DE3) assessed as pup-ylation using Mtb DHFR substrate at 20 uM by SDS-PAGE analysis | Mycobacterium tuberculosis | 1 | assay format | Scientific Literature |
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