# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA5248914 | B | PROTAC activity at VHL/NTMT1 in human HCT-116 cells assessed as reduction in NTMT1 protein level incubated for 24 hrs by Western blot analysis | Homo sapiens | 3 | cell-based format | Scientific Literature | ||
2. | ALA5248915 | B | PROTAC activity at VHL/NTMT1 in human HT-29 cells assessed as reduction in NTMT1 protein level incubated for 24 hrs by Western blot analysis | Homo sapiens | 3 | cell-based format | Scientific Literature | ||
3. | ALA5248916 | B | PROTAC activity at VHL/NTMT1 in human HCT-116 cells assessed as reduction in NTMT1 protein level at 50 uM incubated for 24 hrs by Western blot analysis | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
4. | ALA5248917 | B | PROTAC activity at VHL/NTMT1 in human HT-29 cells assessed as reduction in NTMT1 protein level at 50 uM incubated for 24 hrs by Western blot analysis | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
5. | ALA5248918 | B | PROTAC activity at VHL/NTMT1 in human HCT-116 cells assessed as reduction in NTMT1 protein level by measuring maximal efficacy at 0.1 to 50 uM incubated for 24 hrs by Western blot analysis relative to control | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
6. | ALA5248919 | B | PROTAC activity at VHL/NTMT1 in human HT-29 cells assessed as reduction in NTMT1 protein level by measuring maximal efficacy at 0.1 to 50 uM incubated for 24 hrs by Western blot analysis relative to control | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
7. | ALA5248920 | B | PROTAC activity at VHL/NTMT1 in human HCT-116 cells assessed as reduction in NTMT1 protein level at 50 uM preincubated with MG132 for 2 hrs followed by compound addition and measured after 24 hrs by Western blot analysis | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
8. | ALA5248921 | B | PROTAC activity at VHL/NTMT1 in human HCT-116 cells assessed as reduction in NTMT1 protein level at 50 uM preincubated with MLN4924 for 2 hrs followed by compound addition and measured after 24 hrs by Western blot analysis | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
9. | ALA5248922 | B | PROTAC activity at VHL/NTMT1 in human HCT-116 cells co-expressing eGFP fusion protein assessed as reduction in NTMT1 protein level by measuring reduction in eGFP fluorescence at 100 uM incubated for 24 hrs by fluorescence microscopic analysis | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
10. | ALA5248923 | B | PROTAC activity at VHL/NTMT1 in human HCT-116 cells assessed as reduction in NTMT1 protein level by measuring maximal efficacy at 50 uM measured at 3 hrs by Western blot analysis relative to control | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
11. | ALA5248924 | B | PROTAC activity at VHL/NTMT1 in human HT-29 cells assessed as reduction in NTMT1 protein level at 50 uM measured upto 6 hrs by Western blot analysis | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
12. | ALA5248925 | B | PROTAC activity at VHL/NTMT1 in human HT-29 cells assessed as reduction in NTMT1 protein level by measuring maximal efficacy at 50 uM measured after 24 hrs by Western blot analysis relative to control | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
13. | ALA5248926 | B | PROTAC activity at VHL/NTMT1 in human HT-29 cells assessed as reduction in NTMT1 protein level at 50 uM preincubated with compound for 24 hrs followed by compound washout and measured after 20 hrs by Western blot analysis | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
14. | ALA5248927 | B | PROTAC activity at VHL/NTMT1 in human HCT-116 cells assessed as reduction in NTMT1 protein level at 50 uM preincubated with compound for 24 hrs followed by compound washout and measured after 20 hrs by Western blot analysis | Homo sapiens | 1 | cell-based format | Scientific Literature |