# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA3706257 | Binding | Reductase Assay: All assays were carried out in a reaction buffer containing 100 nM KxPO4 at pH 7.2, 1 mM EDTA, 500 mM KCl and 1 mg/ml BSA. The concentrations of NADPH and HMG-CoA were both 200 μM. The enzyme concentration used is unknown although this concentration is 10-fold lower than that of the stock solution purchased. Inhibitors were dissolved in 75% DMSO. Where inhibitors were found to be insoluble or only partly soluble in 75% DMSO, 100% DMSO was used. Reactions were activated by the addition of enzyme and agitated for 12 seconds following the addition. Absorbance readings were then taken every 20 seconds for 600 seconds. In initial tests the concentration of each inhibitor was set at 50 nM to identify which compounds were the better inhibitors, compared to the known Pravastatin inhibitor. | Rattus norvegicus | 20 | ALA3639298 | single protein format | BindingDB Database | |
2. | ALA615415 | Binding | Ability of compound (2.5 uM) to inhibit the activity of 3-hydroxy -3- methyl glutanyl coenzyme A reductase(HMGR) using [14C]mevalonate in rat liver microsomes | Rattus norvegicus | 1 | ALA1124675 | microsome format | Scientific Literature | |
3. | ALA615416 | Binding | Ability of compound (2500 uM) to inhibit the activity of 3-hydroxy -3- methyl glutanyl coenzyme A reductase(HMGR) using [14C]mevalonate in rat liver microsomes | Rattus norvegicus | 1 | ALA1124675 | microsome format | Scientific Literature | |
4. | ALA615417 | Binding | Ability of compound (250 uM) to inhibit the activity of 3-hydroxy -3- methyl glutanyl coenzyme A reductase(HMGR) using [14C]mevalonate in rat liver microsomes | Rattus norvegicus | 1 | ALA1124675 | microsome format | Scientific Literature | |
5. | ALA615418 | Binding | Ability of compound (25 uM) to inhibit the activity of 3-hydroxy -3- methyl glutanyl coenzyme A reductase(HMGR) using [14C]mevalonate in rat liver microsomes | Rattus norvegicus | 1 | ALA1124675 | microsome format | Scientific Literature | |
6. | ALA615419 | Binding | Ability of compound at 0 uM to inhibit the activity of 3-hydroxy -3- methyl glutanyl coenzyme A reductase(HMGR) using [14C]mevalonate in rat liver microsomes | Rattus norvegicus | 1 | ALA1124675 | microsome format | Scientific Literature | |
7. | ALA615420 | Binding | Ability of compound to inhibit the activity of 3-hydroxy-3-methylglutarylcoenzyme A(HMGR) reductase in rat liver microsomes | Rattus norvegicus | 7 | ALA1124675 | microsome format | Scientific Literature | |
8. | ALA615421 | Binding | Percentage inhibition of 3-hydroxy-3-methyl glutaryl coenzyme A reductase in rat liver microsomes at 250 uM | Rattus norvegicus | 1 | ALA1124675 | microsome format | Scientific Literature | |
9. | ALA615422 | Binding | Percentage inhibition of 3-hydroxy -3- methyl glutanyl coenzyme A reductase(HMGR) in rat liver microsomes at 0 uM | Rattus norvegicus | 1 | ALA1124675 | microsome format | Scientific Literature | |
10. | ALA615423 | Binding | Percentage inhibition of 3-hydroxy -3- methyl glutanyl coenzyme A reductase(HMGR) in rat liver microsomes at 2.5 uM | Rattus norvegicus | 1 | ALA1124675 | microsome format | Scientific Literature | |
11. | ALA872868 | Binding | Percentage inhibition of 3-hydroxy -3- methyl glutanyl coenzyme A reductase(HMGR) in rat liver microsomes at 2500 uM | Rattus norvegicus | 1 | ALA1124675 | microsome format | Scientific Literature | |
12. | ALA615424 | Binding | Percentage inhibition of 3-hydroxy -3- methyl glutanyl coenzyme A reductase(HMGR) in rat liver microsomes at 25 uM | Rattus norvegicus | 1 | ALA1124675 | microsome format | Scientific Literature | |
13. | ALA688066 | Binding | Inhibitory activity against partially purified rat liver HMG-CoA reductase in vitro; 0.0015-0.0040 | 1 | ALA1126959 | assay format | Scientific Literature | ||
14. | ALA688067 | Binding | Inhibitory activity against partially purified rat liver HMG-CoA reductase in vitro; 0.0015-0.0049 | 1 | ALA1126959 | assay format | Scientific Literature | ||
15. | ALA688068 | Binding | Inhibitory activity against partially purified rat liver HMG-CoA reductase in vitro; 0.0040-0.053 | 2 | ALA1126959 | assay format | Scientific Literature | ||
16. | ALA688123 | Binding | Inhibitory activity against partially purified rat liver HMG-CoA reductase in vitro; 0.0050-0.016 | 1 | ALA1126959 | assay format | Scientific Literature | ||
17. | ALA871962 | Binding | Inhibitory activity against partially purified rat liver HMG-CoA reductase in vitro; 0.0073-0.017 | 1 | ALA1126959 | assay format | Scientific Literature | ||
18. | ALA688124 | Binding | Inhibitory activity against partially purified rat liver HMG-CoA reductase in vitro; 0.015-0.51 | 1 | ALA1126959 | assay format | Scientific Literature | ||
19. | ALA688125 | Binding | Inhibitory activity against partially purified rat liver HMG-CoA reductase in vitro; 0.021-0.036 | 2 | ALA1126959 | assay format | Scientific Literature | ||
20. | ALA688126 | Binding | Inhibitory activity against partially purified rat liver HMG-CoA reductase in vitro; 0.026-0.34 | 1 | ALA1126959 | assay format | Scientific Literature |