#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA677230	B	Relative binding affinity calculated as the ratio of molar concentration of Estrogen receptor beta and the triarylpropenones (TAP's) required to decrease [3H]E2 binding by 50%.		25		single protein format	Scientific Literature
2.	ALA829092	B	Inhibition of [3H]17-beta-estradiol binding to mouse ER beta expressed in Escherichia coli	Mus musculus	9		assay format	Scientific Literature
3.	ALA4154493	B	Binding affinity to ERbeta in mouse Melan-a cells	Mus musculus	3		cell-based format	Scientific Literature
4.	ALA4154494	B	Agonist activity at ERbeta in mouse Melan-a cells assessed as increase in melanin content at 40 uM measured after 48 hrs in presence of ER antagonist ICI 182780	Mus musculus	1		cell-based format	Scientific Literature
5.	ALA4190807	B	Activation of ERbeta in mouse calvarial osteoblasts assessed as induction of osteoblast differentiation by measuring increase in ALP activity at 1 pM using PNPP as substrate after 48 hrs by colorimetric method	Mus musculus	4		single protein format	Scientific Literature
6.	ALA4190809	B	Activation of ERbeta in mouse calvarial osteoblasts assessed as induction of mineralization by measuring increase in calcium nodules at 1 pM supplemented with fresh medium containing compounds every 48 hrs for 18 days measured on day 18 by alizarin red-S staining based colorimetric method relative to control	Mus musculus	1		single protein format	Scientific Literature
7.	ALA4190814	B	Up regulation of ERbeta expression in mouse calvarial osteoblasts at 1 to 100 pM after 48 hrs by Western blot analysis	Mus musculus	1		assay format	Scientific Literature
8.	ALA4620361	B	Induction of ESR2-mediated osteoblast differentiation in mouse calvarial osteoblast assessed as increase in ALP activity at 1 nM measured after 48 hrs by spectrophotometric analysis	Mus musculus	1		cell-based format	Scientific Literature
9.	ALA4620362	B	Induction of ESR2-mediated osteoblast differentiation in mouse calvarial osteoblast assessed as increase in ALP activity at 100 pM measured after 48 hrs by spectrophotometric analysis	Mus musculus	1		cell-based format	Scientific Literature
10.	ALA4620363	B	Induction of ESR2-mediated osteoblast differentiation in mouse calvarial osteoblast assessed as increase in ALP activity at 1 pM measured after 48 hrs by spectrophotometric analysis	Mus musculus	4		cell-based format	Scientific Literature
11.	ALA4620366	B	Induction of ESR2-mediated osteoblast differentiation in mouse calvarial osteoblast assessed as formation of mineralized nodule at 1 to 10 uM measured after 21 days by alizarin red staining based assay	Mus musculus	1		cell-based format	Scientific Literature