#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA3429076	Functional	Agonist activity at rat mGluR5 expressed in HEK293 cells assessed as induction of inositol phosphate production at 100 uM by HTRF assay	Rattus norvegicus	1	ALA3425503	cell-based format	Scientific Literature
2.	ALA3429081	Functional	Agonist activity at rat mGluR5 expressed in HEK293 cells assessed as induction of inositol phosphate production by HTRF assay	Rattus norvegicus	1	ALA3425503	cell-based format	Scientific Literature
3.	ALA3600166	Functional	Negative allosteric modulator activity at rat mGlu5 receptor expressed in HEK293 cells assessed as effect on quisqualate-induced inositol phosphate accumulation incubated for 30 mins by florescence assay	Rattus norvegicus	4	ALA3596138	cell-based format	Scientific Literature
4.	ALA3600167	Binding	Potency index, ratio of IC50 for MPEP to IC50 for test compound for negative allosteric modulator activity at rat mGlu5 receptor expressed in HEK293 cells	Rattus norvegicus	1	ALA3596138	cell-based format	Scientific Literature
5.	ALA3600169	Functional	Agonist activity at rat mGlu5 receptor expressed in HEK293 cells by inositol phosphate accumulation assay	Rattus norvegicus	1	ALA3596138	cell-based format	Scientific Literature
6.	ALA3599913	Binding	Displacement of [3H]MPEP from mGluR5 receptor in Sprague-Dawley rat forebrain membrane after 60 mins by liquid scintillation spectrometry	Rattus norvegicus	22	ALA3596098	assay format	Scientific Literature
7.	ALA3607322	Binding	Ago-PAM activity at rat mGlu5 receptor assessed as increase in glutamate-induced response	Rattus norvegicus	1	ALA3603827	single protein format	Scientific Literature
8.	ALA3620961	Functional	Modulation of rat mGlu5 receptor assessed as calcium mobilization by cell based fluorescence assay	Rattus norvegicus	79	ALA3616441	cell-based format	Scientific Literature
9.	ALA3620962	Functional	Modulation of rat mGlu5 receptor assessed as assessed as calcium mobilization at 30 uM by cell based fluorescence assay	Rattus norvegicus	64	ALA3616441	cell-based format	Scientific Literature
10.	ALA3619172	Functional	Positive allosteric modulation of rat mGlu5 receptor assessed as calcium mobilization by cell based fluorescence assay	Rattus norvegicus	40	ALA3616441	cell-based format	Scientific Literature
11.	ALA3619173	Functional	Negative allosteric modulation of rat mGlu5 receptor assessed as calcium mobilization by cell based fluorescence assay	Rattus norvegicus	24	ALA3616441	cell-based format	Scientific Literature
12.	ALA3637034	Binding	Positive allosteric modulation of mGlu5 in rat astrocytes	Rattus norvegicus	1	ALA3632544	cell-based format	Scientific Literature
13.	ALA3637035	Binding	Positive allosteric modulation of mGlu5 in rat astrocytes relative to glutamate	Rattus norvegicus	1	ALA3632544	cell-based format	Scientific Literature
14.	ALA3637425	Functional	Antagonist activity at rat mGluR5 expressed in CHO cells assessed as inhibition of L-glutamate-induced inositol monophosphate accumulation after 1 hr by FRET assay	Rattus norvegicus	4	ALA3632567	cell-based format	Scientific Literature
15.	ALA3636977	Functional	Positive allosteric modulation of rat mGlu5 assessed as calcium mobilization by cell-based assay	Rattus norvegicus	1	ALA3632544	cell-based format	Scientific Literature
16.	ALA3636978	Functional	Positive allosteric modulation of rat mGlu5 assessed as calcium mobilization by cell-based assay relative to glutamate	Rattus norvegicus	1	ALA3632544	cell-based format	Scientific Literature
17.	ALA3636980	Functional	Positive allosteric modulation of rat mGlu5 assessed as maximal calcium mobilization response at 10 uM by cell-based assay relative to control	Rattus norvegicus	1	ALA3632544	cell-based format	Scientific Literature
18.	ALA3705628	Binding	Cell Based Assay: mGluR5 activity was determined in a cell-based assay.	Rattus norvegicus	77	ALA3638497	cell-based format	BindingDB Database
19.	ALA3705718	Binding	Binding Assay: Binding assays were performed as described in Schaffhauser H, Richards J G, Cartmell J, Chaboz S, Kemp J A, Klingelschmidt A, Messer J, Stadler H, Woltering T and Mutel V (1998) In vitro binding characteristics of a new selective group II metabotropic glutamate receptor radioligand, [3H]LY354740, in rat brain. Mol Pharmacol 53:228-233) at room temperature with slight modifications.	Rattus norvegicus	101	ALA3639017	tissue-based format	BindingDB Database
20.	ALA3706001	Binding	Metabotropic Glutamate Receptor Activity Assay: The utility of the compounds in accordance with the present invention as negative allosteric modulators of metabotropic glutamate receptor activity, in particular mGluR5 activity, can be demonstrated by methodology known in the art. Human embryonic kidney (HEK) cells transfected with rat or human mGluR5 were plated in clear bottom assay plates for assay in a Functional Drug Screening System (FDSS). The cells were loaded with a Ca2+-sensitive fluorescent dye (e.g., Fluo-4), and the plates were washed and placed in the FDSS instrument. Test compound was applied to cells 3 seconds after baseline readings were taken. Cells were incubated with the test compounds for 140 seconds and then stimulated with an EC20 concentration of an mGluR5 agonist (e.g., glutamate, 3,5-dihydroxyphenylglycine, or quisqualate); 60-80 seconds later an EC80 concentration of agonist was added and readings taken for an additional 40 seconds. Data were collected at 1 Hz. Negative allosteric modulation of the agonist.	Rattus norvegicus	15	ALA3638661	assay format	BindingDB Database