# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA733331 | B | Inhibition of murine Mitogen-activated protein kinase p38 alpha | Mus musculus | 13 | ALA1146826 | single protein format | Scientific Literature | |
2. | ALA733332 | B | Inhibition of murine phosphorylated His-Mitogen-activated protein kinase p38 alpha. | 18 | ALA1147483 | single protein format | Scientific Literature | ||
3. | ALA733333 | B | Inhibition of murine His-Mitogen-activated protein kinase p38 alpha phosphorylation. | 18 | ALA1147484 | single protein format | Scientific Literature | ||
4. | ALA733334 | B | In vivo inhibition of murine Mitogen-activated protein kinase p38 alpha activity, GST-ATF-2 as substrate in the presence of 120 uM ATP | 17 | ALA1134856 | organism-based format | Scientific Literature | ||
5. | ALA733335 | B | Inhibition of murine p38 alpha kinase | Mus musculus | 12 | ALA1136507 | single protein format | Scientific Literature | |
6. | ALA733336 | B | Kd value and murine Mitogen-activated protein kinase p38 alpha complex was calculated | 27 | ALA1145135 | single protein format | Scientific Literature | ||
7. | ALA733337 | B | Thermal denaturation temperature (Tm) of the inhibitor and murine pMitogen-activated protein kinase p38 alpha complex | 27 | ALA1145135 | single protein format | Scientific Literature | ||
8. | ALA733338 | B | Tested in vitro for filter plate binding versus gamma-[33P]ATP in active recombinant murine FLAG-P38 alpha fusion protein (GST-Activating transcription factor-2). | 18 | ALA1145952 | single protein format | Scientific Literature | ||
9. | ALA735048 | B | Inhibition of [gamma-33P]-ATP binding to recombinant murine p38 alpha at 100 nM | Mus musculus | 1 | ALA1145952 | single protein format | Scientific Literature | |
10. | ALA842191 | B | Inhibition of murine p38 alpha MAP kinase | Mus musculus | 12 | ALA1132910 | single protein format | Scientific Literature | |
11. | ALA863439 | B | Inhibitory activity against murine p38-alpha MAPK | Mus musculus | 20 | ALA1147752 | single protein format | Scientific Literature | |
12. | ALA932044 | B | Inhibition of mouse p38alpha in anisomycin-stimulated RAW 264.7 cells by ELISA | Mus musculus | 36 | ALA1143180 | cell-based format | Scientific Literature | |
13. | ALA1112959 | B | Inhibition of mouse p38alpha after 3 hrs by SPA method | Mus musculus | 12 | ALA1158539 | single protein format | Scientific Literature | |
14. | ALA1825800 | B | Inhibition of p38alpha-dependent LPS-stimulated IL1-beta release in mouse RAW264.7 cells at 12.5 uM after 1 hr by sandwich immunoassay relative to control | Mus musculus | 1 | ALA1821566 | cell-based format | Scientific Literature | |
15. | ALA2090336 | B | Inhibition of mouse p38apha assessed as reduction in GST-ATF2 substrate phosphorylation by SPA assay | Mus musculus | 20 | ALA2086361 | single protein format | Scientific Literature | |
16. | ALA3707920 | B | Fluorescence Quenching Assay: CADD and biological testing to identify compounds that interact with novel ERK substrate docking sites. The known ERK2 docking sites include the DRS (site 1) and FRS (site 5) regions, whereas predicted docking sites are labeled sites 2-4 (Table 6). One compound, 76, that targets the DRS is being marketed by Calbiochem/EMD Biosciences (Cat#328006) as an ERK inhibitor. Successful inhibitor identification to date has been based on a combination of fluorescence quenching (FQ) of ERK2 wild type and docking site mutants in the presence of test compound. | Mus musculus | 5 | ALA3638871 | single protein format | BindingDB Database | |
17. | ALA3877175 | B | Inhibition of mouse N-terminal 5His-tagged p38alpha assessed as decrease in 6His-tagged MK2a (51 to 400 residues) phosphorylation in presence of [gamma-33P]-ATP by scintillation counting method | Mus musculus | 1 | ALA3875233 | single protein format | Scientific Literature | |
18. | ALA4361864 | B | Inhibition of p38alpha MAPK in LPS-stimulated mouse RAW264.7 cells assessed as reduction in p38alpha MAPK phosphorylation at 4 uM incubated for 60 mins by Western blot analysis | Mus musculus | 1 | ALA4359854 | cell-based format | Scientific Literature | |
19. | ALA4361871 | B | Inhibition of p38alpha MAPK in LPS-stimulated mouse RAW264.7 cells assessed as reduction in p38alpha MAPK phosphorylation incubated for 60 mins by Western blot analysis | Mus musculus | 1 | ALA4359854 | cell-based format | Scientific Literature | |
20. | ALA4361872 | B | Inhibition of p38alpha MAPK in LPS-stimulated mouse RAW264.7 cells assessed as reduction in p38alpha MAPK phosphorylation at 2 uM incubated for 60 mins by Western blot analysis | Mus musculus | 1 | ALA4359854 | cell-based format | Scientific Literature |