| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA2051716 | B | Inhibition of N-terminus His6-tagged human kallikrein 6 expressed in baculovirus infected insect sf9 cells using Boc-Phe-Ser-Arg-AMC as substrate by fluorescence analysis | Homo sapiens | 6 | ALA2046411 | cell-based format | Scientific Literature | |
| 2. | ALA3110694 | B | Inhibition of human KLK6 at 1 to 500 uM after 5 mins by spectrophotometry | Homo sapiens | 19 | ALA3108751 | single protein format | Scientific Literature | |
| 3. | ALA3774275 | B | Inhibition of human tissue kallikrein-6 expressed in baculovirus infected insect cells using Abz-KLRSSQ-EDDnp as substrate preincubated for 10 mins followed by substrate addition by FRET assay | Homo sapiens | 1 | ALA3769311 | cell-based format | Scientific Literature | |
| 4. | ALA3776077 | B | Inhibition of human KLK6 using Abz-KLRSSKQ-EDDnp as substrate at >1 uM preincubated for 5 mins followed by substrate addition by spectrofluorimetric analysis | Homo sapiens | 3 | ALA3774332 | single protein format | Scientific Literature | |
| 5. | ALA3776082 | B | Inhibition of human KLK6 using Abz-KLRSSKQ-EDDnp as substrate at 10 uM preincubated for 5 mins followed by substrate addition by spectrofluorimetric analysis | Homo sapiens | 3 | ALA3774332 | single protein format | Scientific Literature | |
| 6. | ALA3776085 | B | Inhibition of human KLK6 using Abz-KLRSSKQ-EDDnp as substrate at 10 to 100 uM preincubated for 5 mins followed by substrate addition by spectrofluorimetric analysis | Homo sapiens | 3 | ALA3774332 | single protein format | Scientific Literature | |
| 7. | ALA4038140 | B | Inhibition of recombinant human C-terminal His-tagged KLK6 isoform 1 (1 to 244 residues) expressed in HEK293 cells using fluorogenic Boc-QARAMC peptide as substrate by fluorescence based assay | Homo sapiens | 3 | ALA4033799 | cell-based format | Scientific Literature | |
| 8. | ALA4201823 | B | Inhibition of KLK6 in human FaDu cells assessed as reduction in cell proliferation incubated for 48 hrs by BrdU incorporation assay | Homo sapiens | 1 | ALA4196210 | cell-based format | Scientific Literature | |
| 9. | ALA4201824 | B | Inhibition of human KLK6 expressed in baculovirus/insect cell line system using Abz-KLRSSKQ-EDDnp substrate | Homo sapiens | 1 | ALA4196210 | assay format | Scientific Literature | |
| 10. | ALA4201825 | B | Inhibition of recombinant human KLK6 assessed as Kinact/Ki ratio using Boc-QAR-AMC substrate | Homo sapiens | 1 | ALA4196210 | single protein format | Scientific Literature | |
| 11. | ALA4201826 | B | Inhibition of His-6-tagged human KLK6 R74G/R76Q/N132Q mutant expressed in Escherichia coli DH10 at 40 uM pre-incubated for 30 mins before N-Boc-FSR-AMC substrate addition and measured after 30 mins | Homo sapiens | 5 | ALA4196210 | assay format | Scientific Literature | |
| 12. | ALA4201827 | B | Inhibition of His-6-tagged human KLK6 R74G/R76Q/N132Q mutant expressed in Escherichia coli DH10 pre-incubated for 30 mins before N-Boc-FSR-AMC substrate addition and measured after 30 mins | Homo sapiens | 43 | ALA4196210 | assay format | Scientific Literature | |
| 13. | ALA4201831 | B | Binding affinity to His-6-tagged human KLK6 R74G/R76Q/N132Q mutant expressed in Escherichia coli DH10 assessed as covalent adduct formation by measuring mass shift at 100 to 200 uM incubated for 1 hr by ESI-MS analysis | Homo sapiens | 2 | ALA4196210 | assay format | Scientific Literature | |
| 14. | ALA4201832 | B | Binding affinity to His-6-tagged human KLK6 R74G/R76Q/N132Q mutant expressed in Escherichia coli DH10 assessed as covalent adduct formation at Ser195 at 100 to 200 uM incubated for 1 hr by ESI-MS analysis | Homo sapiens | 2 | ALA4196210 | assay format | Scientific Literature | |
| 15. | ALA4201833 | B | Binding affinity to His-6-tagged human KLK6 R74G/R76Q/N132Q/S195A mutant expressed in Escherichia coli DH10 assessed as covalent adduct formation at 100 to 200 uM incubated for 1 hr by ESI-MS analysis | Homo sapiens | 2 | ALA4196210 | assay format | Scientific Literature | |
| 16. | ALA4201834 | B | Binding affinity to His-6-tagged human KLK6 R74G/R76Q/N132Q mutant expressed in Escherichia coli DH10 assessed as covalent adduct formation at 100 to 200 uM incubated for 24 hrs by ESI-MS analysis | Homo sapiens | 1 | ALA4196210 | assay format | Scientific Literature | |
| 17. | ALA4201835 | B | Binding affinity to His-6-tagged human KLK6 R74G/R76Q/N132Q mutant expressed in Escherichia coli DH10 assessed as covalent adduct formation at 100 to 200 uM incubated for 48 hrs by ESI-MS analysis | Homo sapiens | 1 | ALA4196210 | assay format | Scientific Literature | |
| 18. | ALA4201836 | B | Binding affinity to His-6-tagged human KLK6 R74G/R76Q/N132Q mutant expressed in Escherichia coli DH10 assessed as covalent adduct formation at 100 to 200 uM incubated for 72 hrs by ESI-MS analysis | Homo sapiens | 1 | ALA4196210 | assay format | Scientific Literature | |
| 19. | ALA4201837 | B | Inhibition of His-6-tagged human KLK6 R74G/R76Q/N132Q mutant expressed in Escherichia coli DH10 assessed as Kinact/Ki ratio pre-incubated for 30 mins before N-Boc-FSR-AMC substrate addition and measured after 30 mins | Homo sapiens | 1 | ALA4196210 | assay format | Scientific Literature | |
| 20. | ALA4201838 | B | Time dependent inhibition of His-6-tagged human KLK6 R74G/R76Q/N132Q mutant expressed in Escherichia coli DH10 pre-incubated up to 40 mins before N-Boc-FSR-AMC substrate addition and measured after 30 mins | Homo sapiens | 1 | ALA4196210 | assay format | Scientific Literature |
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