#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA3706023	Functional	FLIPR Assay: Compounds were tested for their ability to inhibit C5a-mediated calcium mobilization using a stable cell line over expressing the human C5aR & the Galpha 15 protein on the FLIPR (Fluorescent Imaging Plate Reader) Tetra system. Cells were maintained in culture media containing DMEM (Dulbecco's Modified Eagle Medium) with 4.5 g/L glucose, 10% fetal bovine serum, 100 U/mL, 1x non-essential amino acid, and 250 ug/mL G418 (Geneticin). Prior to testing of compounds, cells were plated at 10,000 cells/well in clear bottom 384-well black plate, and incubated overnight at 37C. with 5% CO2. On the day of experiment, culture media was removed, and replaced with assay buffer HBSS (Hanks' Balanced Salt Solution) with 20 mM HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) and 0.1% BSA (bovine serum albumin) BSA containing calcium 5 dye (Molecular Devices) and 2.5 mM probenecid. After 1 hour incubation at 37C., 5% CO2 for 60 min, cells were pre-incubated with compounds for 30 minutes.		143	ALA3639060	assay format	BindingDB Database
2.	ALA3739126	Binding	Displacement of [125I]-C5a from C5aR in human MDM cells at 20 uM after 60 mins by microbeta scintillation counting analysis	Homo sapiens	48	ALA3734743	cell-based format	Scientific Literature
3.	ALA654425	Binding	Inhibition of binding of [125I]C5a to human polymorphonuclear leukocyte(PMN) C5a anaphylatoxin chemotactic receptor		1	ALA1132227	single protein format	Scientific Literature
4.	ALA654426	Binding	Compound was evaluated for inhibition of radiolabeled C5a binding to its membrane C5a anaphylatoxin chemotactic receptor		1	ALA1151577	assay format	Scientific Literature
5.	ALA653615	Binding	Inhibition of radiolabeled C5a binding to its membrane bound C5a anaphylatoxin chemotactic receptor		27	ALA1151577	assay format	Scientific Literature
6.	ALA652972	Functional	Compound was evaluated for the antagonistic activity against C5a anaphylatoxin chemotactic receptor in human neutrophil C5a stimulated respiratory burst assay		1	ALA1129896	assay format	Scientific Literature
7.	ALA652973	Binding	Ability to inhibit binding of radiolabeled C5a anaphylatoxin to intact membrane bound receptor from human neutrophils.		26	ALA1126141	assay format	Scientific Literature
8.	ALA652974	Binding	Compound was tested for its ability to inhibit binding of radiolabeled C5a anaphylatoxin chemotactic receptor to intact membrane bound receptor from human neutrophils.; insoluble		1	ALA1126141	assay format	Scientific Literature
9.	ALA856063	Binding	Compound was tested for its ability to inhibit binding of radiolabeled C5a anaphylatoxin chemotactic receptor to intact membrane bound receptor from human neutrophils; insoluble		1	ALA1126141	assay format	Scientific Literature
10.	ALA652975	Binding	Inhibition of binding of [125I]C5a to human polymorphonuclear leukocyte(PMN) C5a anaphylatoxin chemotactic receptor		1	ALA1132227	single protein format	Scientific Literature
11.	ALA652976	Binding	Inhibitory activity for C5a anaphylatoxin chemotactic receptor binding using radiolabeled C5a receptor on neutrophil membranes (~2.0 uM)		1	ALA1151577	assay format	Scientific Literature
12.	ALA652977	Binding	Inhibitory activity against radiolabeled C5a receptor on neutrophil membranes expressing C5a anaphylatoxin chemotactic receptor		1	ALA1151577	assay format	Scientific Literature
13.	ALA652978	Binding	Tested for its ability to compete with [125I]C5a for binding to C5a anaphylatoxin chemotactic receptor of human neutrophil membrane preparations	Homo sapiens	11	ALA1130566	assay format	Scientific Literature
14.	ALA652979	Binding	Tested for the ability to compete with [125I]-CO13 ([125I]-Y-F-K-A-Cha-G-L-dF-R) for binding to C5a anaphylatoxin chemotactic receptor from human neutrophil membrane preparations		24	ALA1130566	assay format	Scientific Literature
15.	ALA651507	Binding	Tested for the its ability to compete with [125I]-CO13 [125I]-Y-F-K-A-Cha-G-L-dF-R) for binding to C5a anaphylatoxin chemotactic receptor of human neutrophil membrane preparations		1	ALA1130566	assay format	Scientific Literature
16.	ALA655461	Binding	Binding affinity against C5a anaphylatoxin chemotactic receptor assayed on human monocyte cell line U937		20	ALA1129896	cell-based format	Scientific Literature
17.	ALA655462	Binding	Compound was evaluated for its ability to inhibit the binding of [125I]C5a anaphylatoxin chemotactic receptor to polymorphonuclear leukocyte (PMNL) membrane receptor at 1 mM concentration.		4	ALA1126137	assay format	Scientific Literature
18.	ALA874667	Binding	Compound was tested for the inhibition of [125I]C5a anaphylatoxin chemotactic receptor binding to PMNL intact cells at a concentration of 0.1-1 mM.		2	ALA1125281	cell-based format	Scientific Literature
19.	ALA655463	Binding	Compound was tested for the inhibition of [125I]C5a binding to C5a anaphylatoxin chemotactic receptor in PMNL membranes.		1	ALA1125281	cell membrane format	Scientific Literature
20.	ALA655464	Binding	Effect on uM; no effect		5	ALA1126126	single protein format	Scientific Literature