| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA678888 | B | Maximum percent potentiation against gamma-aminobutyric acid (GABA) A receptor, alpha 2 | Xenopus laevis | 33 | single protein format | Scientific Literature | ||
| 2. | ALA678889 | B | Effective concentration against gamma-aminobutyric acid (GABA) A receptor, alpha 2 | Xenopus laevis | 30 | single protein format | Scientific Literature | ||
| 3. | ALA678890 | B | Displacement of [3H]Ro-151788 from human GABA-A receptor alpha2 subunit expressed in L(tk) cells | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 4. | ALA1687496 | F | Agonist activity at recombinant GABAA receptor subunit alpha-2 expressed in CHO cells at 3 uM by sensitive fluorometric read-out assay relative to midazolam | 16 | cell-based format | Scientific Literature | |||
| 5. | ALA1687498 | B | Displacement of [3H]flumazenil from GABAA receptor subunit alpha-2 expressed in CHO cells | 17 | cell-based format | Scientific Literature | |||
| 6. | ALA1687501 | F | Agonist activity at recombinant GABAA receptor subunit alpha-2 expressed in CHO cells at 1 uM by sensitive fluorometric read-out assay relative to midazolam | 1 | cell-based format | Scientific Literature | |||
| 7. | ALA2327261 | B | Positive allosteric modulation of GABAA alpha2 (unknown origin) relative to control | Homo sapiens | 3 | single protein format | Scientific Literature | ||
| 8. | ALA2327955 | B | Positive allosteric modulation of GABAA alpha2 (unknown origin) | Homo sapiens | 4 | single protein format | Scientific Literature | ||
| 9. | ALA3706142 | B | Radioligand Competition Binding Assay: The affinity of the test compounds was determined by radioligand competition binding assay, using the known compound [3H]Ro-15-1788 (Flumazenil) (Perkin Elmer, 85.4 Ci/mmol) and the human recombinant GABA A receptor containing the alpha2, beta2, and gamma3 subunits.Membranes were prepared from HEK cells expressing hGABA A alpha2beta2- gamma3 receptor, and validated to ascertain protein concentration, receptor expression and to determine the Kd of the flumazenil as well as the Ki of a standard set of compounds before being used to test new compounds.The assay was carried out in 96 well plates; testing compounds using a 10 point semi-log dilution range from 19 uM top concentration. 100 ul of radioligand and 100 ul of membrane in 50 mM Tris-HCI and 0.05% F127 with 1 ul of test compound was incubated for 2 hours to allow the reaction to achieve equilibrium, and then harvested onto filter plates, dried and counted on a TopCount NXT. The data was analysed, and the Ki values were presented. | 50 | cell-based format | BindingDB Database | |||
| 10. | ALA4403717 | B | Binding affinity to GABA-A alpha2 (unknown origin) | Homo sapiens | 26 | single protein format | Scientific Literature | ||
| 11. | ALA4403724 | B | Binding affinity to GABA-A alpha2 (unknown origin) assessed as increase in induced current relative to control | Homo sapiens | 23 | single protein format | Scientific Literature | ||
| 12. | ALA4403750 | B | Positive allosteric modulation of GABAA alpha2 (unknown origin) relative to control | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 13. | ALA4403771 | B | Binding affinity to GABA-A alpha2 (unknown origin) assessed as reduction in DMCM induced current relative to DMCM | Homo sapiens | 2 | single protein format | Scientific Literature | ||
| 14. | ALA4403775 | B | Binding affinity to GABA-A alpha2 (unknown origin) assessed as reduction in DMCM induced current relative to control | Homo sapiens | 5 | single protein format | Scientific Literature | ||
| 15. | ALA4701825 | A | Negative allosteric modulation of recombinant human GABA-A alpha2 expressed in HEK293 cells assessed as reduction in GABA-induced current at 1 uM relative to control | Homo sapiens | 25 | cell-based format | Scientific Literature | ||
| 16. | ALA4701850 | A | Positive allosteric modulation of recombinant human GABA-A alpha2 expressed in HEK293 cells assessed as reduction in GABA-induced current at 1 uM relative to control | Homo sapiens | 5 | cell-based format | Scientific Literature | ||
| 17. | ALA4837571 | B | Binding affinity to GABBA alpha2 (unknown origin) | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 18. | ALA5235568 | B | Binding affinity to human recombinant GABAA alpha2 receptor assessed as inhibition constant incubated for 1 hrs by liquid scintillation counting analysis | Homo sapiens | 21 | single protein format | Scientific Literature | ||
| 19. | ALA5235574 | B | Inhibition of human recombinant GABAA alpha2 receptor | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 20. | ALA5235583 | B | Inhibition of GABAA alpha2 receptor (unknown origin) | Homo sapiens | 2 | single protein format | Scientific Literature |
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