#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA678888	B	Maximum percent potentiation against gamma-aminobutyric acid (GABA) A receptor, alpha 2	Xenopus laevis	33	ALA1135642	single protein format	Scientific Literature
2.	ALA678889	B	Effective concentration against gamma-aminobutyric acid (GABA) A receptor, alpha 2	Xenopus laevis	30	ALA1135642	single protein format	Scientific Literature
3.	ALA678890	B	Displacement of [3H]Ro-151788 from human GABA-A receptor alpha2 subunit expressed in L(tk) cells	Homo sapiens	1	ALA1148234	cell-based format	Scientific Literature
4.	ALA1687496	F	Agonist activity at recombinant GABAA receptor subunit alpha-2 expressed in CHO cells at 3 uM by sensitive fluorometric read-out assay relative to midazolam		16	ALA1681636	cell-based format	Scientific Literature
5.	ALA1687498	B	Displacement of [3H]flumazenil from GABAA receptor subunit alpha-2 expressed in CHO cells		17	ALA1681636	cell-based format	Scientific Literature
6.	ALA1687501	F	Agonist activity at recombinant GABAA receptor subunit alpha-2 expressed in CHO cells at 1 uM by sensitive fluorometric read-out assay relative to midazolam		1	ALA1681636	cell-based format	Scientific Literature
7.	ALA2327261	B	Positive allosteric modulation of GABAA alpha2 (unknown origin) relative to control	Homo sapiens	3	ALA2321869	single protein format	Scientific Literature
8.	ALA2327955	B	Positive allosteric modulation of GABAA alpha2 (unknown origin)	Homo sapiens	4	ALA2321869	single protein format	Scientific Literature
9.	ALA3706142	B	Radioligand Competition Binding Assay: The affinity of the test compounds was determined by radioligand competition binding assay, using the known compound [3H]Ro-15-1788 (Flumazenil) (Perkin Elmer, 85.4 Ci/mmol) and the human recombinant GABA A receptor containing the alpha2, beta2, and gamma3 subunits.Membranes were prepared from HEK cells expressing hGABA A alpha2beta2- gamma3 receptor, and validated to ascertain protein concentration, receptor expression and to determine the Kd of the flumazenil as well as the Ki of a standard set of compounds before being used to test new compounds.The assay was carried out in 96 well plates; testing compounds using a 10 point semi-log dilution range from 19 uM top concentration. 100 ul of radioligand and 100 ul of membrane in 50 mM Tris-HCI and 0.05% F127 with 1 ul of test compound was incubated for 2 hours to allow the reaction to achieve equilibrium, and then harvested onto filter plates, dried and counted on a TopCount NXT. The data was analysed, and the Ki values were presented.		50	ALA3638710	cell-based format	BindingDB Database
10.	ALA4403717	B	Binding affinity to GABA-A alpha2 (unknown origin)	Homo sapiens	26	ALA4402545	single protein format	Scientific Literature
11.	ALA4403724	B	Binding affinity to GABA-A alpha2 (unknown origin) assessed as increase in induced current relative to control	Homo sapiens	23	ALA4402545	single protein format	Scientific Literature
12.	ALA4403750	B	Positive allosteric modulation of GABAA alpha2 (unknown origin) relative to control	Homo sapiens	1	ALA4402545	single protein format	Scientific Literature
13.	ALA4403771	B	Binding affinity to GABA-A alpha2 (unknown origin) assessed as reduction in DMCM induced current relative to DMCM	Homo sapiens	2	ALA4402545	single protein format	Scientific Literature
14.	ALA4403775	B	Binding affinity to GABA-A alpha2 (unknown origin) assessed as reduction in DMCM induced current relative to control	Homo sapiens	5	ALA4402545	single protein format	Scientific Literature
15.	ALA4701825	A	Negative allosteric modulation of recombinant human GABA-A alpha2 expressed in HEK293 cells assessed as reduction in GABA-induced current at 1 uM relative to control	Homo sapiens	25	ALA4699487	cell-based format	Scientific Literature
16.	ALA4701850	A	Positive allosteric modulation of recombinant human GABA-A alpha2 expressed in HEK293 cells assessed as reduction in GABA-induced current at 1 uM relative to control	Homo sapiens	5	ALA4699487	cell-based format	Scientific Literature
17.	ALA4837571	B	Binding affinity to GABBA alpha2 (unknown origin)	Homo sapiens	1	ALA4837182	single protein format	Scientific Literature
18.	ALA5235568	B	Binding affinity to human recombinant GABAA alpha2 receptor assessed as inhibition constant incubated for 1 hrs by liquid scintillation counting analysis	Homo sapiens	21	ALA5233266	single protein format	Scientific Literature
19.	ALA5235574	B	Inhibition of human recombinant GABAA alpha2 receptor	Homo sapiens	1	ALA5233266	single protein format	Scientific Literature
20.	ALA5235583	B	Inhibition of GABAA alpha2 receptor (unknown origin)	Homo sapiens	2	ALA5233266	single protein format	Scientific Literature