#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA2065180	B	Binding affinity to CK2beta at 150 uM followed by UV irradiation for 30 mins with Wood's glass filtered medium pressure mercury arc light by SDS-PAGE based pull down assay	Homo sapiens	1	ALA2062417	single protein format	Scientific Literature
2.	ALA3887160	B	Inhibition Assay: Test compounds dissolved and diluted in DMSO (2 μl) were added to a reaction mixture comprising 10 μl of 5× Reaction Buffer (40 mM MOPS pH 7.0, 5 mM EDTA), 10 μl of recombinant human PIM2 solution (4 ng PIM-2 dissolved in dilution buffer (20 mM MOPS pH 7.0; EDTA 1 mM; 5% Glycerol; 0.01% Brij 35; 0.1%; 0.1% 2-mercaptoethanol; 1 mg/ml BSA)) and 8 ul of water. Reactions were initiated by the addition of 10 ul of ATP Solution (49% (15 mM MgCl2; 75 uM ATP) 1% ([γ-33P]ATP: Stock 1 mCi/100 μl; 3000 Ci/mmol (Perkin Elmer)) and 10 ul of substrate peptide solution (RSRSSYPAGT (SEQ ID NO.: 6), dissolved in water at a concentration of 1 mM), Reactions were maintained for 10 min at 30° C. The reactions were quenched with 100 ul of 0.75% Phosphoric acid, then transferred to and filtered through a Phosphocellulose filter plate (Millipore, MSPH-N6B-50). After washing each well 4 times with 0.75% Phosphoric acid, scintillation fluid (20 uL) was added to each well and the resid		532	ALA3886195	single protein format	BindingDB Database
3.	ALA3888221	B	Inhibition Assay: 5 uM ATP: Test compounds dissolved and diluted in DMSO (2 μl) were added to a reaction mixture comprising 10 μl of 5× Reaction Buffer (40 mM MOPS pH 7.0, 5 mM EDTA), 10 μl of recombinant human PIM2 solution (4 ng PIM-2 dissolved in dilution buffer (20 mM MOPS pH 7.0; EDTA 1 mM; 5% Glycerol; 0.01% Brij 35; 0.1%; 0.1% 2-mercaptoethanol; 1 mg/ml BSA)) and 8 ul of water. Reactions were initiated by the addition of 10 ul of ATP Solution (49% (15 mM MgCl2; 5 uM ATP) 1% ([γ-33P]ATP: Stock 1 mCi/100 μl; 3000 Ci/mmol (Perkin Elmer)) and 10 ul of substrate peptide solution (RSRSSYPAGT (SEQ ID NO.: 6), dissolved in water at a concentration of 1 mM), Reactions were maintained for 10 min at 30° C. The reactions were quenched with 100 ul of 0.75% Phosphoric acid, then transferred to and filtered through a Phosphocellulose filter plate (Millipore, MSPH-N6B-50). After washing each well 4 times with 0.75% Phosphoric acid, scintillation fluid (20 uL) was added to each well and		390	ALA3886195	single protein format	BindingDB Database
4.	ALA4118626	B	Binding affinity to CSNK2B in human NCI-H23 cells at 1 uM by mass spectrometry based pull down assay	Homo sapiens	1	ALA4118021	cell-based format	Scientific Literature
5.	ALA4414187	B	Inhibition of CK2beta autophosphorylation (unknown origin) in presence of ATP by radiometric kinase assay	Homo sapiens	3	ALA4411336	single protein format	Scientific Literature
6.	ALA4414206	B	Inhibition of recombinant human GST-tagged CK2beta at 15 uM in presence of varying ATP levels by radiometric kinase assay	Homo sapiens	1	ALA4411337	single protein format	Scientific Literature
7.	ALA4414279	B	Inhibition of recombinant human GST-tagged CK2beta at 15 uM in presence of varying substrate levels by radiometric kinase assay	Homo sapiens	1	ALA4411337	single protein format	Scientific Literature