#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA3428992	Binding	Inhibition of human TIE2/TEK using poly[Glu:Tyr] (4:1) peptide substrate at 1 uM by 33P Hotspot assay	Homo sapiens	1	ALA3425491	single protein format	Scientific Literature
2.	ALA3594870	Binding	Inhibition of TIE2 (unknown origin)	Homo sapiens	1	ALA3593212	single protein format	Scientific Literature
3.	ALA3595406	Binding	Inhibition of human TEK at 0.1 uM relative to control	Homo sapiens	1	ALA3593229	single protein format	Scientific Literature
4.	ALA3603011	Binding	Inhibition of recombinant TIE2 (unknown origin) at 1 uM after 3 hrs	Homo sapiens	1	ALA3600296	single protein format	Scientific Literature
5.	ALA3620320	Binding	Inhibition of TIE2 (unknown origin) at 10 uM after 40 mins by scintillation counting analysis	Homo sapiens	1	ALA3616395	single protein format	Scientific Literature
6.	ALA3631497	Binding	Inhibition of TIE2 (unknown origin) at 10 uM after 120 mins P33 radiolabeled kinase activity assay	Homo sapiens	3	ALA3627608	single protein format	Scientific Literature
7.	ALA3631697	Binding	Inhibition of Tie2 (unknown origin) at 1 uM by FRET method	Homo sapiens	1	ALA3627690	single protein format	Scientific Literature
8.	ALA3631586	Binding	Inhibition of TIE2 (unknown origin) assessed as remaining activity at 10 uM by high-throughput assay relative to control	Homo sapiens	1	ALA3627674	single protein format	Scientific Literature
9.	ALA3707796	Binding	Kinase Screen Assay: KINOMEscan (Ambit Biosciences, San Diego, Calif.), a high-throughput method for screening small molecular agents against a large panel of human kinases, was utilized for compound 2. The technology is a competition binding assay that profiled the selectivity of compound 2 against 350 kinases, each fused to a proprietary tag. The quantity of each kinase bound to an immobilized, active site-directed ligand was measured in the presence and absence of compound 2.	Homo sapiens	1	ALA3639044	single protein format	BindingDB Database
10.	ALA3707700	Binding	Kinase Inhibition Assay: The compound of Example 39, 2-(1H-indol-5-ylamino)-6-(2,4-difluorophenylsulfonyl)-8-methylpyrido[2,3-d]pyrimidin-7(8H)-one, was subjected to a kinase inhibition assay for the kinases. Compounds were tested in 5 dose IC50 mode with 10-fold serial dilution starting at 10 μM. Staurosporine, a known protein kinase inhibitor, was tested in 5-dose IC50 mode with 3-fold serial dilution starting at 20 μM. Reactions were carried out in 10 μM ATP. The results are shown in Table 5. The results show that the compound of Example 39 is a kinase inhibitor highly selective for the kinase Plk2.	Homo sapiens	2	ALA3639034	single protein format	BindingDB Database
11.	ALA3738545	Binding	Inhibition of TIE2 (unknown origin) at 200 nM by electrophoretic mobility shift assay	Homo sapiens	2	ALA3734671	single protein format	Scientific Literature
12.	ALA3750183	Binding	Inhibition of human TIE2 using poly[Glu:Tyr] (4:1) as substrate	Homo sapiens	1	ALA3745707	single protein format	Scientific Literature
13.	ALA3751239	Binding	Inhibition of Tie2 (unknown origin) at 1 uM	Homo sapiens	1	ALA3745631	single protein format	Scientific Literature
14.	ALA3750777	Binding	Inhibition of TIE2 (unknown origin) at 10 uM	Homo sapiens	9	ALA3745672	single protein format	Scientific Literature
15.	ALA3750812	Binding	Inhibition of TIE2 (unknown origin) at 1 uM	Homo sapiens	1	ALA3745672	single protein format	Scientific Literature
16.	ALA3761606	Binding	Inhibition of TEK (unknown origin) at 500 nM	Homo sapiens	11	ALA3758163	single protein format	Scientific Literature
17.	ALA3761276	Binding	Inhibition of TIE2 (unknown origin) at 100 nM	Homo sapiens	1	ALA3758169	single protein format	Scientific Literature
18.	ALA3767332	Binding	Inhibition of TIE2 (unknown origin) at 10 uM	Homo sapiens	1	ALA3763027	single protein format	Scientific Literature
19.	ALA3766483	Binding	Inhibition of human TIE2 using poly(Glu, Tyr) as substrate by [33P]-phosphoryl transfer assay	Homo sapiens	1	ALA3763130	single protein format	Scientific Literature
20.	ALA3772128	Binding	Inhibition of human recombinant TIE2 assessed as residual activity at 10 uM after 1 hr by qPCR analysis relative to control	Homo sapiens	1	ALA3769377	single protein format	Scientific Literature