#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA3427825	Binding	Inhibition of S6K1 (unknown origin) assessed as remaining activity at 100 uM by radioactive filter binding assay in presence of [33P]-ATP relative to control	Homo sapiens	16	ALA3425453	single protein format	Scientific Literature
2.	ALA3428320	Binding	Inhibition of human p70S6K/RPS6KB1 using KKRNRTLTK peptide substrate at 1 uM by 33P Hotspot assay	Homo sapiens	1	ALA3425491	single protein format	Scientific Literature
3.	ALA3595095	Binding	Inhibition of p70S6K (unknown origin)	Homo sapiens	1	ALA3593212	single protein format	Scientific Literature
4.	ALA3595419	Binding	Inhibition of human RPS6KB1 at 0.1 uM relative to control	Homo sapiens	1	ALA3593229	single protein format	Scientific Literature
5.	ALA3603007	Binding	Inhibition of recombinant P70S6K1 (unknown origin) at 1 uM after 3 hrs	Homo sapiens	1	ALA3600296	single protein format	Scientific Literature
6.	ALA3630750	Binding	Inhibition of RPS6KB1 (unknown origin) at 10 uM after 120 mins P33 radiolabeled kinase activity assay	Homo sapiens	3	ALA3627608	single protein format	Scientific Literature
7.	ALA3631181	Binding	Inhibition of p70S6K (unknown origin) at 1 uM by FRET method	Homo sapiens	1	ALA3627690	single protein format	Scientific Literature
8.	ALA3705357	Binding	Enzyme Assay: P70S6K inhibitor compounds are diluted and plated in 96 well plates. A reaction mixture including the following components is then added to the compound plate to initiate the enzyme reaction; P70S6K (3 nM, T412E mutant, Millipore) is mixed with 24 μM ATP in an assay buffer containing 100 mM Hepes (pH 7.5), 5 mM MgCl2, 1 mM DTT, 0.015% Brij and 1 μM of the substrate peptide FITC-AHA-AKRRRLSSLRA-OH (derived from the S6 ribosomal protein sequence, FITC=fluorescein isothiocyanate, AHA=6-aminohexanoic acid). The reaction is incubated for 90 min at 25° C., before the addition of 10 mM EDTA to stop the reaction. The proportion of substrate and product (phosphorylated) peptide is analysed on a Caliper Life Sciences Lab Chip 3000, using a pressure of −1.4 psi, and upstream and downstream voltages of −3000 and −700 respectively.	Homo sapiens	584	ALA3639326	single protein format	BindingDB Database
9.	ALA3734492	Binding	Inhibition of recombinant human P70S6K using fluorescein-labeled peptide as substrate by fluorescence-electrophoretic mobility shift assay	Homo sapiens	1	ALA3727354	single protein format	Patent Bioactivity Data
10.	ALA3737502	Binding	Inhibition of RPS6KB1 (unknown origin) at 1 uM by selectscreen kinase profiling assay in presence of ATP	Homo sapiens	1	ALA3734692	single protein format	Scientific Literature
11.	ALA3742696	Binding	Inhibition of P70S6K (unknown origin)	Homo sapiens	1	ALA3739276	single protein format	Scientific Literature
12.	ALA3751055	Binding	Inhibition of p70S6K (unknown origin) at 1 uM	Homo sapiens	1	ALA3745631	single protein format	Scientific Literature
13.	ALA3748033	Binding	Inhibition of human RPS6KB1 using [KKRNRTLTK] as substrate	Homo sapiens	1	ALA3745707	single protein format	Scientific Literature
14.	ALA3756051	Binding	Inhibition of recombinant human p70S6K assessed as residual activity at 1 uM by radiometric assay in presence of [gamma-33P]-ATP relative to control	Homo sapiens	1	ALA3751668	single protein format	Scientific Literature
15.	ALA3756062	Binding	Inhibition of recombinant human p70S6K by radiometric assay in presence of [gamma-33P]-ATP	Homo sapiens	1	ALA3751668	single protein format	Scientific Literature
16.	ALA3757340	Binding	Inhibition of p70S6K in human TT cells assessed as suppression of S6 phosphorylation at Y317 at 0.1 to 10 uM after 4 hrs by Western blot analysis	Homo sapiens	1	ALA3751668	cell-based format	Scientific Literature
17.	ALA3757341	Binding	Inhibition of p70S6K in human TT cells assessed as suppression of S6 phosphorylation at Y317 at 1 to 10 uM after 4 hrs by Western blot analysis	Homo sapiens	1	ALA3751668	cell-based format	Scientific Literature
18.	ALA3761294	Binding	Inhibition of p70S6K(unknown origin) at 100 nM	Homo sapiens	2	ALA3758169	single protein format	Scientific Literature
19.	ALA3761493	Binding	Inhibition of p70S6K(unknown origin) at 1000 nM	Homo sapiens	1	ALA3758169	single protein format	Scientific Literature
20.	ALA3767324	Binding	Inhibition of P70S6K (unknown origin) at 10 uM	Homo sapiens	1	ALA3763027	single protein format	Scientific Literature