#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA3428407	Binding	Inhibition of p38gamma MAPK (unknown origin) assessed as remaining activity at 100 uM by radioactive filter binding assay in presence of [33P]-ATP relative to control	Homo sapiens	16	ALA3425453	single protein format	Scientific Literature
2.	ALA3630749	Binding	Inhibition of P38gamma (unknown origin) at 10 uM after 120 mins P33 radiolabeled kinase activity assay	Homo sapiens	3	ALA3627608	single protein format	Scientific Literature
3.	ALA3706161	Binding	Enzyme Inhibition Assay: The kinase enzyme binding activities of compounds disclosed herein were determined using a proprietary assay which measures active site-directed competition binding to an immobilized ligand (Fabian, M. A. et al., Nature Biotechnol., 2005, 23:329-336). These assays were conducted by DiscoverX (formerly Ambit; San Diego, Calif.). The Kd value (Dissociation constant value) was calculated as the index of affinity of the compounds to each kinase. The enzyme inhibitory activities of compounds disclosed herein were determined by FRET using synthetic peptides labelled with both donor and acceptor fluorophores (Z-LYTE, Invitrogen Ltd., Paisley, UK). The inhibitory activities of compounds of the invention against p38MAPKgamma (MAPK12: Invitrogen), were evaluated in a similar fashion to that described hereinabove. The enzyme (800 ng/mL, 2.5 uL) was incubated with the test compound (2.5 uL at either 4 ug/mL, 0.4 ug/mL, 0.04 ug/mL, or 0.004 ug/mL) for 2 hr at RT.	Homo sapiens	1	ALA3639240	single protein format	BindingDB Database
4.	ALA3707751	Binding	Kinase Screen Assay: KINOMEscan (Ambit Biosciences, San Diego, Calif.), a high-throughput method for screening small molecular agents against a large panel of human kinases, was utilized for compound 2. The technology is a competition binding assay that profiled the selectivity of compound 2 against 350 kinases, each fused to a proprietary tag. The quantity of each kinase bound to an immobilized, active site-directed ligand was measured in the presence and absence of compound 2.	Homo sapiens	1	ALA3639044	single protein format	BindingDB Database
5.	ALA3707643	Binding	Fluorescence Resonance Energy Transfer (FRET) Assay: The enzyme inhibitory activity of test compounds was determined by fluorescence resonance energy transfer (FRET) using synthetic peptides labelled with both donor and acceptor fluorophores (Z-LYTE, Invitrogen). Recombinant, phosphorylated p38 MAPK (MAPK12:Millipore) was diluted in HEPES buffer, mixed with compound at the desired final concentrations and incubated for 2 hr at RT. The FRET peptide (2 uM) and ATP (100 uM) were next added to the enzyme/compound mixture and incubated for 1 hr. Development reagent (protease) was added for 1 hr prior to detection in a fluorescence microplate reader. The site-specific protease only cleaves non-phosphorylated peptide and eliminates the FRET signal. Phosphorylation levels of each reaction were calculated using the ratio of coumarin emission (donor) over fluorescein emission (acceptor) with high ratios indicating high phosphorylation levels and low ratios indicating low phosphorylation levels.	Homo sapiens	36	ALA3639041	single protein format	BindingDB Database
6.	ALA3742697	Binding	Inhibition of p38gamma (unknown origin)	Homo sapiens	1	ALA3739276	single protein format	Scientific Literature
7.	ALA3751248	Binding	Inhibition of p38 gamma (unknown origin) at 1 uM	Homo sapiens	1	ALA3745631	single protein format	Scientific Literature
8.	ALA3751307	Binding	Inhibition of p38gamma (unknown origin)	Homo sapiens	1	ALA3745712	single protein format	Scientific Literature
9.	ALA3748032	Binding	Inhibition of human P38gamma using MBP as substrate	Homo sapiens	1	ALA3745707	single protein format	Scientific Literature
10.	ALA3761293	Binding	Inhibition of p38gamma(unknown origin) at 100 nM	Homo sapiens	1	ALA3758169	single protein format	Scientific Literature
11.	ALA3777764	Binding	Inhibition of N-terminal His6-tagged recombinant human SAPK3 expressed in baculovirus infected insect Sf9 cells assessed as remaining activity at 1 uM relative to control	Homo sapiens	3	ALA3774357	cell-based format	Scientific Literature
12.	ALA3783187	Binding	Inhibition of human recombinant p38 gamma assessed as phosphorylation of MAPKAP-K2 preincubated for 2 hrs followed by FRET peptide and MAPKAP-K2 addition measured after 1 hr by Z-LYTE assay	Homo sapiens	30	ALA3779765	single protein format	Scientific Literature
13.	ALA3802274	Binding	Inhibition of full length wild type human p38gamma expressed in bacterial system assessed as enzyme activity at 1 uM relative to control	Homo sapiens	1	ALA3797029	assay format	Scientific Literature
14.	ALA3817602	Binding	Inhibition of human P38gamma at 1 uM using MBP as substrate	Homo sapiens	1	ALA3813650	single protein format	Scientific Literature
15.	ALA3816102	Binding	Inhibition of full length recombinant human His-tagged p38gamma MAP kinase expressed in baculovirus expression system at 1 uM relative to control	Homo sapiens	1	ALA3813614	assay format	Scientific Literature
16.	ALA3815732	Binding	Inhibition of SAPK3 (unknown origin) assessed as enzyme activity at 1 uM using 33P ATP by radioactive filter binding assay	Homo sapiens	4	ALA3813616	single protein format	Scientific Literature
17.	ALA3820506	Binding	Inhibition of full length recombinant human GST-tagged SAPK3 expressed in Escherichia coli at 1 uM by radiometric assay relative to control	Homo sapiens	2	ALA3817791	assay format	Scientific Literature
18.	ALA3830956	Binding	Inhibition of human p38gamma MAPK (1 to 367 residues) assessed as remaining enzyme activity at 50 uM after 30 mins by 33P-ATP filter-binding assay	Homo sapiens	1	ALA3826966	single protein format	Scientific Literature
19.	ALA827897	Binding	Inhibition of Stress activated protein kinase 3		1	ALA1143565	single protein format	Scientific Literature
20.	ALA830663	Binding	Inhibition of Stress activated protein kinase 3 at 10 uM with 100 uM ATP		1	ALA1137112	single protein format	Scientific Literature