#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA3619994	Binding	Inhibition of recombinant human activated-MMP1 using Mca-Lys-Pro-Leu-Gly-Leu-Dap(Dnp)-Ala-Arg-NH2 as fluorogenic substrate incubated for 4 hrs followed by substrate addition measured every 10 secs for 20 mins by fluorometric assay	Homo sapiens	8	ALA3616369	single protein format	Scientific Literature
2.	ALA3706226	Binding	Enzyme Assay: The molecules are tested in dose-response studies on the following enzymes MMP1, MMP3, MMP9, ADAMS and ADAM10 according to the same protocol as that described for the TACE enzyme in example 28 but with different substrates (MMP R&D system reference: P126-990 and ADAM R&D system reference: ES003).		4	ALA3639220	single protein format	BindingDB Database
3.	ALA3707929	Binding	Enzymatic Assay: The products are solubilized in DMSO at a concentration of 10 mM. A serial 3-fold dilution over 10 points is carried out so as to have a concentration range of from 10 uM to 0.5 nM final concentration. The TACE enzyme is an internal production (carried out according to the publication protein Eng Des Sel 2006, 19,155-161) and is added so as to have a signal equivalent to 6 times the background noise in 2 h at 37C. The reaction is carried out in 50 mM Tris buffered medium containing 4% glycerol, pH 7.4. The fluorescent substrate is MCA-Pro-Leu-Ala-Val-(Dpa)-Arg-Ser-Ser-Arg-NH2 (R&D systems, reference: ES003). The substrate is cleaved by the enzyme between the alanine and the valine, thus releasing a fluorescent peptide (excitation: 320 nm, emission: 420 nm). The substrate is used at 40 uM. The reaction is carried out in a final volume of 10 ul (4 ul inhibitor, 4 ul substrate, 2 ul enzyme) in a low volume 384-well plate (Corning reference: 3676).		1	ALA3639319	single protein format	BindingDB Database
4.	ALA3707701	Binding	Inhibition Assay: The inhibition tests and the evaluation of the inhibition constants (Ki) on the various MMPs were carried out as described by Devel et al. (Devel et al. 2006 J. Biol. Chem. (7)).		13	ALA3638630	single protein format	BindingDB Database
5.	ALA3755720	Binding	Inhibition of human recombinant MMP1 expressed in Escherichia coli using (5-FAM/QXLTM) FRET peptide as substrate	Homo sapiens	1	ALA3751715	assay format	Scientific Literature
6.	ALA3761866	Binding	Inhibition of full length human recombinant MMP1 using MCA-Arg-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-Glu-Arg-NH2 as substrate preincubated for 60 mins followed by substrate addition by fluorescence assay	Homo sapiens	1	ALA3758105	single protein format	Scientific Literature
7.	ALA3773912	Binding	Inhibition of human MMP-1 using Mca-Lys-Pro-Leu-Gly-Leu-Dap(Dnp)-Ala-Arg-NH2 as substrate after 3 hrs by fluorometric assay	Homo sapiens	4	ALA3769331	single protein format	Scientific Literature
8.	ALA3830610	Binding	Inhibition of human recombinant MMP1 by colorimetric analysis	Homo sapiens	16	ALA3826960	single protein format	Scientific Literature
9.	ALA662793	Binding	Inhibition of radiolabeled rat skin type I collagen degradation by purified human lung fibroblast collagenase.		18	ALA1126554	single protein format	Scientific Literature
10.	ALA662794	Binding	Inhibition of rat skin type I collagen degradation by purified human lung fibroblast collagenase	Homo sapiens	6	ALA1126554	single protein format	Scientific Literature
11.	ALA662795	Binding	Inhibition of rat skin type I collagen degradation by purified human lung fibroblast collagenase without beta-mercaptoethanol	Homo sapiens	3	ALA1126554	single protein format	Scientific Literature
12.	ALA662796	Binding	Inhibition of degradation of radiolabeled rat skin type I collagen by semipurified human lung fibroblast collagenase		31	ALA1127489	single protein format	Scientific Literature
13.	ALA662798	Binding	Inhibition of human recombinant interstitial collagenase MMP-1 at 100 uM	Homo sapiens	1	ALA1128777	single protein format	Scientific Literature
14.	ALA662799	Binding	Inhibition of human fibroblast collagenase	Homo sapiens	14	ALA1127037	single protein format	Scientific Literature
15.	ALA662800	Binding	In vitro inhibition of human fibroblast collagenase at a 10 uM.		19	ALA1127884	single protein format	Scientific Literature
16.	ALA662801	Binding	Inhibition of human recombinant interstitial collagenase MMP-1 at 10 uM	Homo sapiens	9	ALA1128777	single protein format	Scientific Literature
17.	ALA662802	Binding	Inhibition of human fibroblast collagenase at pH 6.5 and at 10 uM	Homo sapiens	1	ALA1127037	single protein format	Scientific Literature
18.	ALA662803	Binding	Inhibition of human skin fibroblast Collagenase at pH 7.5	Homo sapiens	1	ALA1125066	single protein format	Scientific Literature
19.	ALA662804	Binding	Inhibition of human skin fibroblast Collagenase at pH 7.5	Homo sapiens	1	ALA1125066	single protein format	Scientific Literature
20.	ALA662805	Binding	Assayed against human skin fibroblast Collagenase using the spectrophotometric assay with the thiol ester substrate at pH 6.5.		25	ALA1125066	single protein format	Scientific Literature