#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA3588430	Binding	Displacement of [3H]-PGE2 from human EP2 receptor overexpressed in human ECV304 cell membranes by scintillation proximity assay	Homo sapiens	54	ALA3585259	cell-based format	Scientific Literature
2.	ALA3588435	Functional	Antagonist activity at human EP2 receptor overexpressed in human ECV304 cells assessed as inhibition of prostaglandin E2-induced cAMP production	Homo sapiens	54	ALA3585259	cell-based format	Scientific Literature
3.	ALA3588445	Binding	Partial agonist activity at human EP2 receptor overexpressed in human ECV304 cells	Homo sapiens	3	ALA3585259	cell-based format	Scientific Literature
4.	ALA3603414	Binding	Displacement of [3H]-PGE2 from human EP2 receptor by liquid scintillation counting analysis	Homo sapiens	1	ALA3600314	single protein format	Scientific Literature
5.	ALA3602018	Functional	Antagonist activity at recombinant human EP2 receptor expressed in CHO cells assessed as inhibition of PGE2-stimulated cAMP accumulation at 1 uM by scintillation proximity assay in presence of [125I]-cAMP	Homo sapiens	1	ALA3600314	cell-based format	Scientific Literature
6.	ALA3602019	Functional	Agonist activity at recombinant human EP2 receptor expressed in CHO cells assessed as induction of cAMP accumulation at 1 uM after 15 mins by scintillation proximity assay in presence of [125I]-cAMP	Homo sapiens	1	ALA3600314	cell-based format	Scientific Literature
7.	ALA3705179	Binding	Binding Assay: Measurement of EP2 receptor binding affinity was carried out according to the method of Abramovitz et al. (Biochimica et Biophysica Acta, 1483, 285 (2000)). A test compound dissolved in dimethylsulfoxide and [3H]PGE2 (NET-428, available from PerkinElmer Inc.) (final concentration: 10 nM) were added to a buffer solution (10 mM MES-KOH (pH 6.0), 10 mM MgCl2, 1 mM EDTA) in which 10 μg of a membrane fraction (ES-562-M, available from Euroscreen S.A.) of HEK293 cells expressing human EP2 receptor had been suspended, followed by incubation at 30° C. for 60 minutes. The membrane fraction was recovered on glass fiber filter paper (GF/B, available from Whatman PLC) using a cell harvester (M30R, available from Brandel Inc.), and after washing with a buffer solution (10 mM MES-KOH (pH 6.0), 10 mM MgCl2), radioactivity was measured with a liquid scintillation analyzer (2000CA, available from Packard).	Homo sapiens	12	ALA3638739	cell membrane format	BindingDB Database
8.	ALA3707731	Binding	In Vitro Binding Assay: hEP1 and hEP4 membranes are prepared from recombinant HEK293 cells stably expressing the human EP1 (Genbank accession number AY275470) or EP4 (Genbank accession number AY429109) receptors. hEP2 and hEP3 membranes are prepared from HEK293 cells transiently transfected with EP2 (Genbank accession number AY275471) or EP3 (isoform VI: Genbank accession number AY429108) receptor plasmids. Frozen cell pellets are homogenized in homogenization buffer using a Teflon/glass homogenizer. Membrane protein is aliquoted and quick frozen on dry ice prior to storage at -80 C. Homogenization buffer contained 10 mM Tris-HCl, pH 7.4, 250 mM sucrose, 1 mM EDTA, 0.3 mM indomethacin and plus Complete, with EDTA, obtained from Roche Molecular Biochemicals (Catalog Number 1 697 498).Kd values for [3]H-PGE2 binding to each receptor are determined by saturation binding studies or homologous competition. Compounds are tested in a 96-well format using a three-fold dilution series.	Homo sapiens	1	ALA3638628	cell-based format	BindingDB Database
9.	ALA3707870	Binding	In Vitro Binding Assay: hEP1 and hEP4 membranes are prepared from recombinant HEK293 cells stably expressing human EP1 (Genbank accession number AY275470) or EP4 (Genbank accession number AY429109) receptors. hEP2 and hEP3 membranes are prepared from HEK293 cells transiently transfected with EP2 (Genbank accession number AY275471) or EP3 (isoform VI: Genbank accession number AY429108) receptor plasmids. Frozen cell pellets are homogenized in homogenization buffer using a Teflon/glass homogenizer. Membrane protein is aliquoted and quick frozen on dry ice prior to storage at -80 C. Homogenization buffer contained 10 mM Tris-HCl, pH 7.4, 250 mM sucrose, 1 mM EDTA, 0.3 mM indomethacin and plus Complete, with EDTA, obtained from Roche Molecular Biochemicals (Catalog Number 1 697 498). Kd values for [3H]-PGE2 binding to each receptor are determined by saturation binding studies or homologous competition. Compounds are tested in a 96-well format using a three-fold dilution series to generate a 10-point curve.	Homo sapiens	1	ALA3638377	cell-based format	BindingDB Database
10.	ALA3707635	Binding	In Vitro Binding Assay: hEP1 and hEP4 membranes are prepared from recombinant HEK293 cells stably expressing the human EP1 (Genbank accession number AY275470) or EP4 (Genbank accession number AY429109) receptors. hEP2 and hEP3 membranes are prepared from HEK293 cells transiently transfected with EP2 (Genbank accession number AY275471) or EP3 (isoform VI: Genbank accession number AY429108) receptor plasmids. Frozen cell pellets are homogenized in homogenization buffer using a Teflon/glass homogenizer. Membrane protein is aliquoted and quick frozen on dry ice prior to storage at -80 C. Homogenization buffer contained 10 mM Tris-HCl, pH 7.4, 250 mM sucrose, 1 mM EDTA, 0.3 mM indomethacin and plus Complete, with EDTA, obtained from Roche Molecular Biochemicals (Catalog Number 1 697 498).Kd values for [3H]-PGE2 binding to each receptor are determined by saturation binding studies or homologous competition. Compounds are tested in a 96-well format using a three-fold dilution series.	Homo sapiens	1	ALA3639244	cell-based format	BindingDB Database
11.	ALA3744264	Binding	Antagonist activity at human EP2 receptor	Homo sapiens	1	ALA3739413	single protein format	Scientific Literature
12.	ALA3754958	Binding	Binding affinity to human EP2 receptor	Homo sapiens	1	ALA3751833	single protein format	Scientific Literature
13.	ALA3757317	Binding	Agonist activity at human EP2 receptor	Homo sapiens	12	ALA3751723	single protein format	Scientific Literature
14.	ALA3778386	Binding	Displacement of [3H]PGE2 from human recombinant prostanoid EP2 receptor expressed in HEK293 cells at 10'-5 M	Homo sapiens	2	ALA3774372	cell-based format	Scientific Literature
15.	ALA3779739	Binding	Displacement of [3H]PGE2 from human recombinant prostanoid EP2 receptor expressed in HEK293 cells	Homo sapiens	1	ALA3774372	cell-based format	Scientific Literature
16.	ALA3779684	Binding	Binding affinity to human EP2 receptor at 10 uM by radioligand binding assay	Homo sapiens	1	ALA3774360	single protein format	Scientific Literature
17.	ALA3794958	Binding	Binding affinity to human EP2 receptor by competitive binding assay	Homo sapiens	1	ALA3792323	single protein format	Scientific Literature
18.	ALA3794972	Functional	Agonist activity at human EP2 receptor expressed in CHO cells assessed as cAMP level by HTRF assay	Homo sapiens	21	ALA3792278	cell-based format	Scientific Literature
19.	ALA3794973	Functional	Agonist activity at human EP2 receptor expressed in CHO cells assessed as cAMP level by HTRF assay relative to PGE2	Homo sapiens	21	ALA3792278	cell-based format	Scientific Literature
20.	ALA3794974	Binding	Agonist activity at human EP2 receptor expressed in HEK293 cells after 24 hrs beta-arrestin recruitment assay	Homo sapiens	21	ALA3792278	cell-based format	Scientific Literature