#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA931514	B	Inhibition of TrxR1 in human MCF7 cell lysates at 5.0 uM by insulin assay	Homo sapiens	1	ALA1147045	cell-based format	Scientific Literature
2.	ALA1023576	B	Activity at human recombinant TrxR1 at pH 7.4	Homo sapiens	1	ALA1151465	single protein format	Scientific Literature
3.	ALA1023593	B	Ratio of kcat to Km for human recombinant TrxR1 at pH 7.4	Homo sapiens	1	ALA1151465	single protein format	Scientific Literature
4.	ALA1031235	B	Inhibition of human recombinant TrxR1 at pH 7.4	Homo sapiens	1	ALA1151465	single protein format	Scientific Literature
5.	ALA1110820	B	Inhibition of thioredoxin reductase 1		1	ALA1156161	single protein format	Scientific Literature
6.	ALA3399657	B	Inhibition of oxidized human TXR1 reductase activity by insulin reduction assay	Homo sapiens	1	ALA3396965	single protein format	Scientific Literature
7.	ALA3705973	B	In vitro Assay: The method of determining thioredoxin activity is based on the reduction of insulin by thioredoxin. Thioredoxin is reconstituted is by thioredoxin reductase, with NADPH. The resulting free thiol -SH groups react with 5,5′-dithiobis-2-nitrobenzoic acid (DTNB). The termination of the reaction results in the formation of a red colour. The intensity of colouration is determined spectrophotometrically at 412 nm and this corresponds to the number of reduced sulfahydryl groups. The reaction was performed at 37° C. over 30 min. in a buffer containing 50 mM Tris-HCl and 20 mM EDTA at a pH of 7.6. The substrate concentrations used were: 0.25 μM human recombinant thioredoxin and 0.325 μM rat recombinant thioredoxin reductase (IMCO Corporation Ltd AB, Sweden) and 316 μM of insulin, 0.8 μM NADPH, 8 mM DTNB (Sigma Aldrich).		7	ALA3638411	single protein format	BindingDB Database
8.	ALA4008620	B	Inhibition of thioredoxin reductase in human HCT116 cell lysate using thioredoxin as substrate at 0.0001 to 1 uM after 16 hrs by end point insulin assay method	Homo sapiens	7	ALA4007411	assay format	Scientific Literature
9.	ALA4008621	B	Inhibition of thioredoxin reductase in human HCT116 cell lysate using thioredoxin as substrate after 16 hrs by end point insulin assay method	Homo sapiens	4	ALA4007411	assay format	Scientific Literature
10.	ALA4008622	B	Inhibition of thioredoxin reductase in human HCT116 cell lysate using thioredoxin as substrate at 40 to 70 uM after 16 hrs by end point insulin assay method	Homo sapiens	3	ALA4007411	assay format	Scientific Literature
11.	ALA4118689	B	Binding affinity to TXNRD1 in human NCI-H23 cells at 1 uM by mass spectrometry based pull down assay	Homo sapiens	1	ALA4118021	cell-based format	Scientific Literature
12.	ALA4118867	B	Binding affinity to TXNRD1 in human NCI-H358 cells at 1 uM by mass spectrometry based pull down assay	Homo sapiens	1	ALA4118021	cell-based format	Scientific Literature
13.	ALA4134738	B	Inhibition of TrxR1 in human HL60 cells incubated for 12 hrs by TRFS-green dye based fluorescence assay	Homo sapiens	1	ALA4130472	cell-based format	Scientific Literature
14.	ALA4134739	B	Inhibition of TrxR1 in human HL60 cells incubated for 24 hrs by endpoint insulin reduction assay	Homo sapiens	1	ALA4130472	cell-based format	Scientific Literature
15.	ALA4142444	B	Inhibition of TrxR in human HL60 cells using thioredoxin as substrate up to 24 hrs measured for 5 mins by UV-Vis spectrophotometric analysis	Homo sapiens	2	ALA4138191	cell-based format	Scientific Literature
16.	ALA4142445	B	Inhibition of TrxR in human HL60 cells assessed as residual activity at 2 times IC50 using thioredoxin as substrate up to 24 hrs measured for 5 mins by UV-Vis spectrophotometric analysis relative to control	Homo sapiens	2	ALA4138191	cell-based format	Scientific Literature
17.	ALA4142488	B	Inhibition of TrxR in human HL60 cells assessed as residual activity at 1.5 using thioredoxin as substrate measured for 5 mins by UV-Vis spectrophotometric analysis	Homo sapiens	2	ALA4138191	cell-based format	Scientific Literature
18.	ALA4256352	B	Inhibition of NADPH-reduced TrxR1 in human GM02152 cells assessed as suppression of insulin reduction by measuring enzyme rate using Trx as substrate pretreated for 30 mins followed by Trx addition for 30 mins and subsequent eosin-labelled bovine insulin addition measured every 5 mins up to 15 mins by fluorescence assay relative to control	Homo sapiens	1	ALA4251733	cell-based format	Scientific Literature
19.	ALA4256363	B	Inhibition of NADPH-reduced TrxR1 in human GM02152 cells assessed as suppression of insulin reduction by measuring enzyme rate using Trx as substrate pretreated for 30 mins followed by Trx addition for 30 mins and subsequent eosin-labelled bovine insulin addition measured every 5 mins for 15 to 45 mins by fluorescence assay relative to control	Homo sapiens	1	ALA4251733	cell-based format	Scientific Literature
20.	ALA4273141	B	Inhibition of TrxR in human SGC7901 cells	Homo sapiens	1	ALA4270560	cell-based format	Scientific Literature