| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA884712 | B | Ability to compete with radioactive ligands specific to the Serotonergic 5-HT receptor at 10 uM; NA means compound failed to compete | 1 | single protein format | Scientific Literature | |||
| 2. | ALA619199 | F | Receptor-linked G protein activation at 5-hydroxytryptamine receptor was determined by measuring the stimulation of [35S]GTP-gamma-S, binding | 1 | assay format | Scientific Literature | |||
| 3. | ALA619200 | F | Receptor-linked G protein activation at 5-hydroxytryptamine receptor was determined by measuring the stimulation of [35S]GTP-gamma-S, binding (Experiment 1) | 2 | assay format | Scientific Literature | |||
| 4. | ALA619201 | F | Receptor-linked G protein activation at 5-hydroxytryptamine receptor was determined by measuring the stimulation of [35S]GTP-gamma-S, binding (Experiment 2) | 2 | assay format | Scientific Literature | |||
| 5. | ALA619202 | F | Tested for 5-hydroxytryptamine receptor uptake | 1 | assay format | Scientific Literature | |||
| 6. | ALA734876 | F | In vivo percent increase in seizure threshold in mouse MEST model 1 hour following 10 mg/kg p.o. | Mus musculus | 20 | organism-based format | Scientific Literature | ||
| 7. | ALA788342 | F | In vivo percent increase in seizure threshold in mouse MEST model 4 hours following 10 mg/kg p.o. | Mus musculus | 13 | organism-based format | Scientific Literature | ||
| 8. | ALA3887406 | B | Radioligand Binding Assay: The detailed experimental protocols for the radioligand and functional receptor assays are available on the NIMH PDSP website at http://pdsp.med.unc.edu/UNC-CH %20Protocol %20Book.pdf. A. Serotonin receptors: 5-HT1A, 5-HT1B, 5-HT1D, 5-HT1E, 5-HT2A, 5-HT2B, 5-HT2C, 5-HT3, 5-HT5A, 5-HT6 and 5-HT7. Assay Buffer: Standard Binding Buffer (50 mM Tris-HCl, 10 mM MgCl2, 1 mM EDTA, pH 7.4) Membrane Fraction Source: Transiently or stably transfected cell lines (e.g., HEK293, COS, CHO, NIH3T3). Protocol adapted from Roth et al. (1986), J. Pharmacol. Exp. Ther., 238(2): 480-485; and Roth et al. (1994), J. Pharmacol. Exp. Ther., 268(3): 1403-1410. Adrenergic Receptors: alpha1A, alpha1B, alpha2A, alpha2B, alpha2C, beta1, beta2, beta3 Assay Buffers: For alpha1 receptors, alpha1 Binding Buffer (20 mM Tris-HCl, 145 mM NaCl, pH 7.4); for alpha2 receptors, alpha2 Binding Buffer (50 mM Tris-HCl, 5 mM MgCl2, pH 7.7); for beta receptors. | 3 | assay format | BindingDB Database |
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