#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA884712	B	Ability to compete with radioactive ligands specific to the Serotonergic 5-HT receptor at 10 uM; NA means compound failed to compete		1	ALA1135318	single protein format	Scientific Literature
2.	ALA619199	F	Receptor-linked G protein activation at 5-hydroxytryptamine receptor was determined by measuring the stimulation of [35S]GTP-gamma-S, binding		1	ALA1132717	assay format	Scientific Literature
3.	ALA619200	F	Receptor-linked G protein activation at 5-hydroxytryptamine receptor was determined by measuring the stimulation of [35S]GTP-gamma-S, binding (Experiment 1)		2	ALA1132717	assay format	Scientific Literature
4.	ALA619201	F	Receptor-linked G protein activation at 5-hydroxytryptamine receptor was determined by measuring the stimulation of [35S]GTP-gamma-S, binding (Experiment 2)		2	ALA1132717	assay format	Scientific Literature
5.	ALA619202	F	Tested for 5-hydroxytryptamine receptor uptake		1	ALA1127581	assay format	Scientific Literature
6.	ALA734876	F	In vivo percent increase in seizure threshold in mouse MEST model 1 hour following 10 mg/kg p.o.	Mus musculus	20	ALA1132398	organism-based format	Scientific Literature
7.	ALA788342	F	In vivo percent increase in seizure threshold in mouse MEST model 4 hours following 10 mg/kg p.o.	Mus musculus	13	ALA1132398	organism-based format	Scientific Literature
8.	ALA3887406	B	Radioligand Binding Assay: The detailed experimental protocols for the radioligand and functional receptor assays are available on the NIMH PDSP website at http://pdsp.med.unc.edu/UNC-CH %20Protocol %20Book.pdf. A. Serotonin receptors: 5-HT1A, 5-HT1B, 5-HT1D, 5-HT1E, 5-HT2A, 5-HT2B, 5-HT2C, 5-HT3, 5-HT5A, 5-HT6 and 5-HT7. Assay Buffer: Standard Binding Buffer (50 mM Tris-HCl, 10 mM MgCl2, 1 mM EDTA, pH 7.4) Membrane Fraction Source: Transiently or stably transfected cell lines (e.g., HEK293, COS, CHO, NIH3T3). Protocol adapted from Roth et al. (1986), J. Pharmacol. Exp. Ther., 238(2): 480-485; and Roth et al. (1994), J. Pharmacol. Exp. Ther., 268(3): 1403-1410. Adrenergic Receptors: alpha1A, alpha1B, alpha2A, alpha2B, alpha2C, beta1, beta2, beta3 Assay Buffers: For alpha1 receptors, alpha1 Binding Buffer (20 mM Tris-HCl, 145 mM NaCl, pH 7.4); for alpha2 receptors, alpha2 Binding Buffer (50 mM Tris-HCl, 5 mM MgCl2, pH 7.7); for beta receptors.		3	ALA3886311	assay format	BindingDB Database