#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA831701	B	Inhibition of JNK-stimulating phosphatase-1 (JSP-1)	Homo sapiens	27	ALA1140251	single protein format	Scientific Literature
2.	ALA887465	B	Inhibition of GST-tagged human recombinant JSP1	Homo sapiens	23	ALA1144617	single protein format	Scientific Literature
3.	ALA965560	B	Inhibition of GST-tagged PTP MKPX at 100 ug/mL		2	ALA1151767	single protein format	Scientific Literature
4.	ALA1767974	B	Inhibition of human DUSP22	Homo sapiens	1	ALA1765042	single protein format	Scientific Literature
5.	ALA1804670	B	Inhibition of VHX		1	ALA1800013	single protein format	Scientific Literature
6.	ALA2321063	B	Inhibition of VHX (unknown origin) expressed in Escherichia coli using pNPP substrate after 5 mins by spectrophotometric analysis	Homo sapiens	3	ALA2311454	assay format	Scientific Literature
7.	ALA2400542	B	Inhibition of DUSP22 (unknown origin) using DiFMUP as substrate at 5 uM relative to control	Homo sapiens	2	ALA2396625	single protein format	Scientific Literature
8.	ALA3226172	B	Inhibition of human GST-tagged full length MKPX assessed as DiFMUP hydrolysis by fluorometric kinetic-based assay	Homo sapiens	6	ALA3217656	single protein format	Scientific Literature
9.	ALA3226178	B	Inhibition of human GST-tagged full length MKPX assessed as DiFMUP hydrolysis at 10 uM by fluorometric kinetic-based assay	Homo sapiens	6	ALA3217656	single protein format	Scientific Literature
10.	ALA3430031	B	Inhibition of recombinant VHX (unknown origin) using OMFP as substrate after 1 hr by fluorescence assay	Homo sapiens	1	ALA3425500	single protein format	Scientific Literature
11.	ALA3637702	B	Inhibition of GST-tagged MKPX (unknown origin) at 10 uM by plate reader analysis using DiFMUP as substrate	Homo sapiens	5	ALA3632539	single protein format	Scientific Literature
12.	ALA3637706	B	Inhibition of GST-tagged MKPX (unknown origin) by plate reader analysis using DiFMUP as substrate	Homo sapiens	1	ALA3632539	single protein format	Scientific Literature
13.	ALA3772336	B	Inhibition of VHX (unknown origin) expressed in Escherichia coli using pNPP as substrate at 200 uM after 5 mins by spectrophotometry	Homo sapiens	1	ALA3769383	assay format	Scientific Literature
14.	ALA3887782	B	PTP pNPP Assay: PTP activity was assayed using p-nitrophenyl phosphate (pNPP) as a substrate in DMG buffer (50 mM DMG, pH 7.0, 1 mM EDTA, 150 mM NaCl, 2 mM DTT, 0.1 mg/mL BSA) at 25° C. The assays were performed in 96-well plates. Normally, to determine the IC50 values, the reaction was initiated by the addition of enzyme (final concentration of 10 nM) to a reaction mixture (0.2 mL) containing 2 mM (Km for the substrate) pNPP with various concentrations of inhibitors. The reaction rate was measured using a Spectra Max Plus 384 Microplate Spectrophotometer (Molecular Devices). For compound 10, the final TC-PTP concentration was 0.04 nM. The reactions were incubated at room temperature for 30 min and quenched with 5N NaOH. The absorbance at 405 nm was read on the SpectraMax Plus 384 Microplate Spectrophotometer. For the reversibility test, the enzyme was incubated with the inhibitor at room temperature for 30 min before pNPP was added to initiate the reaction. The reaction was then incubated at room temperature.	Homo sapiens	1	ALA3886449	single protein format	BindingDB Database
15.	ALA3888474	B	Enzyme Kinetic Assay: PTP activity was assayed using p-nitrophenyl phosphate (pNPP) as a substrate in 3,3-dimethylglutarate buffer (50 mM 3,3-dimethylglutarate, pH 7.0, 1 mM EDTA, 150 mM NaCl, 2 mM DTT, 0.1 mg/mL BSA) at 25° C. The assays were performed in 96-well plates. Normally, to determine the IC50 values, the reaction was initiated by the addition of enzyme (final concentration at 10 nM) to a reaction mixture (0.2 mL) containing 2 mM (Km for the substrate) pNPP with various concentrations of inhibitors. The reaction rate was measured using a SpectraMax Plus 384 Microplate Spectrophotometer (Molecular Devices). To determine the mode of inhibition, the reactions were initiated by the addition of PTP-MEG2 to the reaction mixtures (0.2 mL) containing various concentrations of pNPP with different concentrations of the inhibitor 7.	Homo sapiens	1	ALA3886725	single protein format	BindingDB Database
16.	ALA4417347	B	Inhibition of LMWPTP (unknown origin) using DiFMUP as substrate incubated for 30 mins followed by substrate addition at pH 6.5 by standard phosphatase assay based fluorescence analysis	Homo sapiens	2	ALA4414670	single protein format	Scientific Literature
17.	ALA4417355	B	Inhibition of LMWPTP (unknown origin) using DiFMUP as substrate at 100 uM incubated for 30 mins followed by substrate addition at pH 6.5 by standard phosphatase assay based fluorescence analysis	Homo sapiens	3	ALA4414670	single protein format	Scientific Literature
18.	ALA4417867	B	Inhibition of DUSP22 (unknown origin) assessed as change in enzyme activity at 150 uM using PNPP substrate incubated for 15 mins relative to control	Homo sapiens	1	ALA4417628	single protein format	Patent Bioactivity Data
19.	ALA4712045	B	Inhibition of DUSP22 (unknown origin) pre-incubated for 20 mins followed by fluorescence substrate addition and measured after 120 mins by DiFMUP assay	Homo sapiens	1	ALA4706627	single protein format	Scientific Literature