| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA833475 | B | In vitro inhibitory concentration against epithelial fatty acid binding protein(E-FABP) | Homo sapiens | 7 | single protein format | Scientific Literature | ||
| 2. | ALA869162 | B | Binding affinity to human kFABP | Homo sapiens | 6 | single protein format | Scientific Literature | ||
| 3. | ALA887936 | B | Displacement of 1,8-ANS from eFABP by fluorescence based-assay | 20 | single protein format | Scientific Literature | |||
| 4. | ALA1102420 | B | Displacement of radiolabeled 1-anilinonaphthalene 8-sulfonic acid from EFABP expressed in Escherichia coli BL21 (DE3) by fluorescence spectrophotometry | 1 | assay format | Scientific Literature | |||
| 5. | ALA5253219 | B | Inhibition of FABP5 (unknown origin) assessed as inhibition constant | Homo sapiens | 4 | single protein format | Scientific Literature | ||
| 6. | ALA3404796 | B | Inhibition of FABP5 (unknown origin) | Homo sapiens | 2 | single protein format | Scientific Literature | ||
| 7. | ALA3828897 | B | Displacement of 1,8-ANS from His6-tagged FABP5 (unknown origin) expressed in Escherichia coli BL21(DE3) cells at 25 uM by fluorescence assay relative to control | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 8. | ALA3861356 | B | Displacement of Bodipy-labeled fatty acid from human N-terminal His6-tagged FABP5 (127 to 132 residues) expressed in Escherichia coli after 30 mins by TR-FRET assay | Homo sapiens | 9 | assay format | Scientific Literature | ||
| 9. | ALA3888116 | B | Time Resolved-Fluorescence Energy Transfer (TR-FRET) Assay: Stock DMSO solutions (1.8 mM) of compounds were serially diluted 3-fold for ten concentrations with 100% DMSO (50 uM to 0.003 uM final compound concentration). 1 ul of these compound dilutions and 1 ul of Bodipy labeled fatty acid 4.5 uM in 100% DMSO (Bodipy FL C11, cat. no. D3862, Invitrogen) were sequentially pipetted in wells of 384-well black polypropylene plates (Thermo Matrix cat. no. 4344). FABP4 or FABP5 protein was then added (28 ul of 64 nM protein in 25 mM Tris pH 7.5, 0.4 mg/ml γ-globulin, 1 mM DTT, 0.012% NP40, final protein concentration: 50 nM). Assay blanks contained ligand, but no protein. Neutral controls contained ligand, but no compound. After adding the detection reagent (Tb antiHis6 antibody, Columbia Biosciences, TB-110, 6 ul of a 24 nM Ab solution in 25 mM Tris pH 7.5, 0.4 mg/ml γ-globulin, final Tb antiHis6 Ab concentration: 4 nM), plates were spun one minute at 1000 rpm. Following an incubation at room temperature with shaking for 30 minutes, plates were read using an Envision reader (Perkin Elmer, Extinction wavelength: 340 nm, Emission: 490 nm and 520 nm, time delay: 100 us; time window: 200 us, 50 flashes). Final assay conditions were: 50 nM FABP protein, 125 nM Bodipy labeled fatty acid, 0.009% (vol/vol) NP40, 5.5% (vol/vol) DMSO in a total final assay volume of 36 ul. The assay was performed in triplicate. | 16 | single protein format | BindingDB Database | |||
| 10. | ALA3888564 | B | Time Resolved-Fluorescence Energy Transfer (TR-FRET) Assay: Stock DMSO solutions (1.8 mM) of compounds were serially diluted 3-fold for ten concentrations with 100% DMSO (50 μM to 0.003 μM final compound concentration). 1 μl of these compound dilutions and 1 μl of Bodipy labeled fatty acid 4.5 μM in 100% DMSO (Bodipy FL C11, cat. no. D3862, Invitrogen) were sequentially pipetted in wells of 384-well black polypropylene plates (Thermo Matrix cat. no. 4344). FABP4 or FABP5 protein was then added (28 μl of 64 nM protein in 25 mM Tris pH 7.5, 0.4 mg/ml α-globulin, 1 mM DTT, 0.012% NP40, final protein concentration: 50 nM). Assay blanks contained ligand, but no protein. Neutral controls contained ligand, but no compound. After adding the detection reagent (Tb antiHis6 antibody, Columbia Biosciences, TB-110, 6 μl of a 24 nM Ab solution in 25 mM Tris pH 7.5, 0.4 mg/ml γ-globulin, final Tb antiHis6 Ab concentration: 4 nM), plates were spun one minute at 1000 rpm. | 42 | single protein format | BindingDB Database | |||
| 11. | ALA4271807 | B | Displacement of DAUDA from recombinant human N-terminal His-tagged FABP5 expressed in Escherichia coli BL21(DE3) after 20 mins by fluorescence assay | Homo sapiens | 47 | assay format | Scientific Literature | ||
| 12. | ALA4271813 | B | Displacement of NBD-stearate from recombinant human N-terminal His-tagged FABP5 expressed in Escherichia coli BL21(DE3) by fluorescence assay | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 13. | ALA4378789 | B | Binding affinity to FABP5 (unknown origin) at 1:1 ratio by fluorescence based thermal shift assay | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 14. | ALA4399642 | B | Displacement of 1,8-ANS from human recombinant full length N-terminal his-tagged FABP5 expressed in Escherichia coli BL21 (DE3) at 25 uM incubated for 3 mins by fluorescence based assay relative to control | Homo sapiens | 35 | assay format | Scientific Literature | ||
| 15. | ALA4399644 | B | Displacement of 1,8-ANS from human recombinant full length N-terminal his-tagged FABP5 expressed in Escherichia coli BL21 (DE3) incubated for 3 mins by fluorescence based assay | Homo sapiens | 23 | assay format | Scientific Literature | ||
| 16. | ALA4399650 | B | Displacement of 1,8-ANS from human recombinant full length N-terminal his-tagged FABP5 expressed in Escherichia coli BL21 (DE3) assessed as inhibition of fluorescence at 100 uM incubated for 3 mins by fluorescence based assay relative to control | Homo sapiens | 2 | assay format | Scientific Literature | ||
| 17. | ALA4424999 | B | Displacement of NBD-Stearate from recombinant human FABP5 measured after 30 mins by fluorescence polarization assay | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 18. | ALA4605259 | B | Displacement of 1,8-ANS from recombinant human 6His-tagged FABP5 expressed in Escherichia coli BL21 DE3 incubated for 15 mins followed by 1,8-ANS addition and measured after 3 mins by fluorescence based assay | Homo sapiens | 12 | assay format | Scientific Literature | ||
| 19. | ALA4721801 | B | Covalent binding affinity to FABP5 at Cys67 residue in human NCI-H358 cells assessed as log2 H/L ratio at 10 uM pretreated for 3 hrs followed by N-(((6S,7R)-7-(4-chloro-3-fluorophenyl)-1,4-oxazepan-6-yl)methyl)-2-hydroxyacetamide treatment for 3 hrs by competitive TMT click chemistry pull-down experiment based LC-MS/MS analysis | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 20. | ALA4832571 | B | Displacement of 1,8-ANS from FABP5 (unknown origin) at 25 uM incubated for 3 mins by fluorescence based analysis relative to control | Homo sapiens | 30 | single protein format | Scientific Literature |
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