#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA3588380	Binding	Inhibition of ATR (unknown origin) at 30 uM relative to control	Homo sapiens	1	ALA3585264	single protein format	Scientific Literature
2.	ALA3705235	Binding	ATR Inhibition Assay: Assays were carried out in a mixture of 50 mM Tris/HCl (pH 7.5), 10 mM MgCl2 and 1 mM DTT. Final substrate concentrations were 10 μM [γ-33P]ATP (3mCi 33P ATP/mmol ATP, Perkin Elmer) and 800 μM target peptide (ASELPASQPQPFSAKKK). Assays were carried out at 25° C. in the presence of 5 nM full-length ATR. An assay stock buffer solution was prepared containing all of the reagents listed above, with the exception of ATP and the test compound of interest. 13.5 μL of the stock solution was placed in a 96 well plate followed by addition of 2 μL of DMSO stock containing serial dilutions of the test compound (typically starting from a final concentration of 15 μM with 3-fold serial dilutions) in duplicate (final DMSO concentration 7%). The plate was pre-incubated for 10 minutes at 25° C. and the reaction initiated by addition of 15 μL [γ-33P]ATP (final concentration 10 μM). The reaction was stopped after 24 hours by the addition of 30 μL 0.1M phosphoric acid containing 2 mM ATP. A multiscreen phosphocellulose filter 96-well plate (Millipore, Cat no. MAPHN0B50) was pretreated with 100 μL 0.2M phosphoric acid prior to the addition of 45 μL of the stopped assay mixture. The plate was washed with 5×200 μL 0.2M phosphoric acid. After drying, 100 μL Optiphase 'SuperMix' liquid scintillation cocktail (Perkin Elmer) was added to the well prior to scintillation counting (1450 Microbeta Liquid Scintillation Counter, Wallac).	Homo sapiens	1	ALA3638439	single protein format	BindingDB Database
3.	ALA3705063	Binding	Cellular Assay: ATM and ATR have distinct and overlapping responses to DNA damage. They must participate together and responses must be co-ordinated. Both pathways may be activated by ionising radiation, however only ATR is activated by UV. Since UV treatment is not practical for use in a high throught-put cell assay, the UV mimetic 4NQ0 (Sigma) was chosen to activate the ATR DNA damage response pathway.	Homo sapiens	16	ALA3638805	single protein format	BindingDB Database
4.	ALA3705064	Binding	Enzyme Assay: ATR for use in the in vitro enzyme assay was obtained from HeLa nuclear extract (CIL Biotech, Mons, Belgium) by immunoprecipitation with rabbit polycolonal antiserum raised to amino acids 400-480 of ATR (Tibbetts R S et, al, 1999, GenesDev.13:152-157).	Homo sapiens	3	ALA3638805	single protein format	BindingDB Database
5.	ALA3705648	Binding	Inhibition Assay: Compounds were screened for their ability to inhibit ATR kinase using a radioactive-phosphate incorporation assay. Assays were carried out in a mixture of 50 mM Tris/HCl (pH 7.5), 10 mM MgCl2 and 1 mM DTT. Final substrate concentrations were 10 uM [gamma-33P]ATP (3 mCi 33P ATP/mmol ATP, Perkin Elmer) and 800 uM target peptide (ASELPASQPQPFSAKKK).Assays were carried out at 25 C. in the presence of 5 nM full-length ATR. An assay stock buffer solution was prepared containing all of the reagents listed above, with the exception of ATP and the test compound of interest. 13.5 uL of the stock solution was placed in a 96 well plate followed by addition of 2 uL of DMSO stock containing serial dilutions of the test compound (typically starting from a final concentration of 15 uM with 3-fold serial dilutions) in duplicate (final DMSO concentration 7%). The plate was pre-incubated for 10 minutes at 25 C.	Homo sapiens	1	ALA3638582	single protein format	BindingDB Database
6.	ALA3705961	Binding	Inhibition Assay: Compounds were screened for their ability to inhibit ATR kinase using a radioactive-phosphate incorporation assay. Assays were carried out in a mixture of 50 mM Tris/HCl (pH 7.5), 10 mM MgCl2 and 1 mM DTT. Final substrate concentrations were 10 μM [γ-33P]ATP (3mCi 33P ATP/mmol ATP, Perkin Elmer) and 800 μM target peptide (ASELPASQPQPFSAKKK).Assays were carried out at 25° C. in the presence of 5 nM full-length ATR. An assay stock buffer solution was prepared containing all of the reagents listed above, with the exception of ATP and the test compound of interest. 13.5 μL of the stock solution was placed in a 96 well plate followed by addition of 2 μL of DMSO stock containing serial dilutions of the test compound (typically starting from a final concentration of 15 μM with 3-fold serial dilutions) in duplicate (final DMSO concentration 7%). The plate was pre-incubated for 10 minutes at 25° C. and the reaction initiated by addition of 15 μL [γ-33P]ATP.	Homo sapiens	2	ALA3638647	single protein format	BindingDB Database
7.	ALA874431	Binding	Inhibition of ATR kinase using rabbit polyclonal antisera	Oryctolagus cuniculus	1	ALA1141182	single protein format	Scientific Literature
8.	ALA827225	Binding	Inhibition of Ataxia telangiectasia related protein ATR kinase		3	ALA1138727	single protein format	Scientific Literature
9.	ALA832926	Binding	Inhibition of ATR kinase using rabbit polyclonal antisera; No inhibition observed at 100 uM	Oryctolagus cuniculus	3	ALA1141182	single protein format	Scientific Literature
10.	ALA860293	Binding	Inhibitory activity against ATR protein kinase receptor at 100 uM	Homo sapiens	23	ALA1141870	single protein format	Scientific Literature
11.	ALA1107748	Binding	Inhibition of ATR		1	ALA1154286	single protein format	Scientific Literature
12.	ALA1104618	Binding	Inhibition of ATR by DELFIA assay		11	ALA1157009	single protein format	Scientific Literature
13.	ALA1226705	Binding	Inhibition of Rad3-related (ATR) protein-mediated p53 phosphorylation		1	ALA1221185	single protein format	Scientific Literature
14.	ALA1767585	Binding	Inhibition of full length recombinant ATR after 24 hrs by radiometric phosphate incorporation assay		51	ALA1765064	single protein format	Scientific Literature
15.	ALA1767685	Binding	Inhibition of ATR in human HT-29 cells assessed as hydroxyurea-induced phosphorylation of H2AX by immunofluorescence microscopy	Homo sapiens	17	ALA1765064	cell-based format	Scientific Literature
16.	ALA1767695	Binding	Competitive inhibition of full length recombinant ATR Morrison equation analysis		1	ALA1765064	single protein format	Scientific Literature
17.	ALA4352750	Binding	Inhibition of recombinant human full-length N-terminal Flag epitope-tagged ATR expressed in HEK293T cells using ASELPASQPQPFSAKKK peptide as substrate measured after 24 hrs in presence of [gamma-33P] ATP by microbeta liquid scintillation counting analysis	Homo sapiens	19	ALA4350970	cell-based format	Scientific Literature
18.	ALA4352755	Binding	Inhibition of ATR in human HCT116 cells assessed as reduction in histone H2AX phosphorylation by Hoechst staining-based immunofluorescence microscopic analysis	Homo sapiens	18	ALA4350970	cell-based format	Scientific Literature
19.	ALA3367299	Binding	Activation of ATR in human PC3 cells assessed as ChK1 phosphorylation at Ser296 at 20 uM after 12 hrs by western blot analysis	Homo sapiens	1	ALA3297648	cell-based format	Scientific Literature
20.	ALA3367300	Binding	Activation of ATR in human DU145 cells assessed as ChK1 phosphorylation at Ser296 at 20 uM after 12 hrs by western blot analysis	Homo sapiens	1	ALA3297648	cell-based format	Scientific Literature