#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA3424980	Binding	Inhibition of human recombinant N-terminal His6-tagged IGF-1R using fluorescence substrate after 80 mins by caliper off-chip incubation mobility shift assay	Homo sapiens	21	ALA3421608	single protein format	Scientific Literature
2.	ALA3428091	Binding	Inhibition of IGF-1R (unknown origin) assessed as remaining activity at 100 uM by radioactive filter binding assay in presence of [33P]-ATP relative to control	Homo sapiens	16	ALA3425453	single protein format	Scientific Literature
3.	ALA3580036	Binding	Inhibition of human IGF-1R assessed as residual activity at 10 uM relative to control	Homo sapiens	1	ALA3576773	single protein format	Scientific Literature
4.	ALA3583129	Binding	Inhibition of Tel-fused IGF1R (unknown origin) expressed in mouse BA/F3 cells after 48 hrs by luciferase reporter gene assay in absence of recombinant mouse IL3	Homo sapiens	3	ALA3580662	cell-based format	Scientific Literature
5.	ALA3594871	Binding	Inhibition of IGF1R (unknown origin)	Homo sapiens	1	ALA3593212	single protein format	Scientific Literature
6.	ALA3595425	Binding	Inhibition of human IGF1R at 0.1 uM relative to control	Homo sapiens	1	ALA3593229	single protein format	Scientific Literature
7.	ALA3599531	Binding	Inhibition of IGF-1R (unknown origin) at 0.5 uM relative to control	Homo sapiens	3	ALA3596148	single protein format	Scientific Literature
8.	ALA3606976	Binding	Inhibition of IGF1R (unknown origin) at 250 nM by radioactive filter-binding assay	Homo sapiens	1	ALA3603808	single protein format	Scientific Literature
9.	ALA3611084	Binding	Inhibition of IGF1R (unknown origin) at 100 nM	Homo sapiens	1	ALA3608257	single protein format	Scientific Literature
10.	ALA3620002	Binding	Inhibition of IGF1R (unknown origin) at 10 uM after 40 mins by scintillation counting analysis	Homo sapiens	2	ALA3616395	single protein format	Scientific Literature
11.	ALA3620032	Binding	Inhibition of IGF1R (unknown origin) after 40 mins by scintillation counting analysis	Homo sapiens	1	ALA3616395	single protein format	Scientific Literature
12.	ALA3624353	Binding	Inhibition of human recombinant IGF-1R	Homo sapiens	1	ALA3621115	single protein format	Scientific Literature
13.	ALA3631830	Binding	Inhibition of IGF1R (unknown origin) at 1 uM by FRET method	Homo sapiens	1	ALA3627690	single protein format	Scientific Literature
14.	ALA3631253	Binding	Inhibition of IGF1R (unknown origin) at 10 uM after 120 mins P33 radiolabeled kinase activity assay	Homo sapiens	3	ALA3627608	single protein format	Scientific Literature
15.	ALA3636790	Binding	Inhibition of IGF1R (unknown origin) after 120 mins by HotSpot assay	Homo sapiens	1	ALA3632549	single protein format	Scientific Literature
16.	ALA3705323	Binding	DELFIA assay: The kinase activity is measured by DELFIA assay (dissociation-enhanced lanthanide fluorescence immunoassay, Perkin Elmer). The cytoplasmic kinase domain of human IGF-1R (amino acids 964-1370) is expressed as a fusion protein with a glutathione-S-transferase tag (IGF-1R-GST) in High Five Cells (Invitrogen). Enzyme activity is measured in the presence of substances and a control substance. Poly-glutamate-tyrosine peptide (pEY, Sigma Aldrich) and biotinylated pEY (bio-pEY) are used as reaction substrates. 10 uL of substance in 25% DMSO are mixed with 30 uL of IGF-1R-GST solution (67 mM HEPES pH 7.4, 15 ug/mL pEY, 1.7 ug/mL bio-pEY, 13.3 mM MgCl2, 3.3 mM dithiothreitol, 0.0033% Brij 35, 2 ng IGF-1R-GST) in 96-well plates. The reactions are started with 10 uL of a 750 uM ATP solution. After 40 min at RT the reactions are stopped with 50 uL of stop solution (250 mM EDTA, 20 mM HEPES pH 7.4).	Homo sapiens	419	ALA3639315	single protein format	BindingDB Database
17.	ALA3705353	Binding	Inhibition Assay: Inhibition assay using IGF1R activity in a cell free kinase assay.	Homo sapiens	10	ALA3638490	single protein format	BindingDB Database
18.	ALA3705546	Binding	Tyrosine Kinase Assay: Kinase assay using IGF1-receptor.	Homo sapiens	45	ALA3638492	single protein format	BindingDB Database
19.	ALA3705429	Binding	Biochemical Assay: The inhibitory activity of putative kinase inhibitors and the potency of selected compounds were determined using a trans-phosphorylation assay.A specific substrate was incubated with the kinase in appropriate buffer conditions in the presence of ATP traced with .sup.33P-.gamma.-ATP (gamma phosphate-labeled, Redivue.TM. Code Number AH9968, 1000-3000 Ci/mmole, Amersham Biosciences Piscataway, N.J., USA), optimal cofactors and test compound.At the end of the phosphorylation reaction, more than 98% cold and radioactive ATP were captured by an excess of Dowex ion exchange resin. The resin was allowed to settle to the bottom of reaction wells by gravity. Supernatant, containing substrate peptide, was subsequently withdrawn and transferred into a counting plate, and radioactivity (corresponding to phosphate incorporated into peptide) was evaluated by .beta.-counting. Assay Conditions:The kinase assay was run with a final enzyme concentration of 6 nM, in the presence of 6 microM ATP, 1 nM .	Homo sapiens	4	ALA3638444	single protein format	BindingDB Database
20.	ALA3707956	Binding	In Vitro Kinase Activity Assay: Compounds to be tested were dissolved in 100% DMSO in a range of concentrations from 10-8 to 10-3 M including negative control (DMSO), then diluted with Kinase Buffer (50 mM HEPES, pH 7.5, 1 mM EGTA, 10 mM MgCl2, 0.01% Tween-20, and 2 mM DTT, added fresh) to 4x the final concentration. The final curve included 11 concentrations (10-11 to 10-5 M) plus negative control DMSO tested in triplicate.Kinase and ATP concentrations necessary for optimal activity were determined in separate experiments. (Table 2). Briefly, increasing concentrations of kinase were incubated in a fixed concentration of ATP (200 uM) in Kinase Buffer to determine maximal activity at various time points (30-120 min). The concentration of kinase that resulted in maximal activity with the shortest incubation time was then incubated in Kinase Buffer with increasing concentrations of ATP.	Homo sapiens	1	ALA3639188	single protein format	BindingDB Database