#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA3705108	Binding	ALDH2 Assay: Standard ALDH2 reaction mixtures contained 150 uM formaldehyde, 2.5 mM NAD+, 10 mM MgCl2 and 10 nM recombinant human ALDH2 in 50 mM Hepes buffer, pH 7.4, 0.01% Tween 20 in a final volume of 50 ul using 384-well plates. After 60 min of pre-incubation of compound with ALDH2 and formaldehyde, the reaction was started by adding NAD+ and the reaction mixture was allowed to proceed for 90 minutes. Activity of the enzyme was determined by monitoring NADH formation using Perkin-Elmer Envision Reader with excitation and emission wavelengths set at 340 and 460 nm, respectively.	Homo sapiens	41	ALA3638670	single protein format	BindingDB Database
2.	ALA3706196	Binding	Inhibition Assay: Standard ALDH2 reaction mixtures contained 150 uM formaldehyde, 2.5 mM NAD+, 10 mM MgCl2 and 10 nM recombinant human ALDH2 in 50 mM Hepes buffer, pH 7.4, 0.01% Tween 20 in a final volume of 50 ul using 384-well plates. After 60 min of pre-incubation of compound with ALDH2 and formaldehyde, the reaction was started by adding NAD+ and the reaction mixture was allowed to proceed for 90 minutes. Activity of the enzyme was determined by monitoring NADH formation using Perkin-Elmer Envision Reader with excitation and emission wavelengths set at 340 and 460 nm, respectively.	Homo sapiens	14	ALA3638424	single protein format	BindingDB Database
3.	ALA641008	Binding	Inhibition of Hamster Liver mitochondrial ALDH-2	Cricetulus griseus	12	ALA1133504	single protein format	Scientific Literature
4.	ALA641178	Functional	Compound was evaluated for the inhibition of Hamster Liver mitochondrial ALDH-2	Cricetulus griseus	1	ALA1133504	assay format	Scientific Literature
5.	ALA641179	Binding	Compound was evaluated for the inhibition of Hamster Liver mitochondrial ALDH-2; No inhibition	Cricetulus griseus	5	ALA1133504	single protein format	Scientific Literature
6.	ALA641141	Binding	Inhibition of hamster liver aldehyde dehydrogenase ALDH-2	Cricetulus griseus	43	ALA1134620	assay format	Scientific Literature
7.	ALA641142	Binding	Inhibition of hamster liver aldehyde dehydrogenase ALDH-2; No inhibition uM	Cricetulus griseus	13	ALA1134620	assay format	Scientific Literature
8.	ALA693590	Functional	Accumulation of 5 HIAL in isolated Hamster Liver Mitochondria at 0.3 uM.	Cricetulus griseus	18	ALA1133504	mitochondrion format	Scientific Literature
9.	ALA693591	Functional	Accumulation of 5 HIAL in isolated Hamster Liver Mitochondria at 0.9 uM.	Cricetulus griseus	18	ALA1133504	mitochondrion format	Scientific Literature
10.	ALA693592	Functional	Accumulation of 5 HIAL in isolated Hamster Liver Mitochondria at 3 uM.	Cricetulus griseus	17	ALA1133504	mitochondrion format	Scientific Literature
11.	ALA693593	Functional	Accumulation of 5 HIAL in isolated Hamster Liver Mitochondria at 9 uM.	Cricetulus griseus	18	ALA1133504	mitochondrion format	Scientific Literature
12.	ALA3421191	Binding	Inhibition of recombinant human ALDH2 using propionaldehyde as substrate preincubated for 2 mins with NAD+ followed by substrate addition by UV-Vis spectrophotometric analysis	Homo sapiens	5	ALA3414499	single protein format	Scientific Literature
13.	ALA3421192	Binding	Inhibition of recombinant human ALDH2 using propionaldehyde as substrate at 100 uM preincubated for 2 mins with NAD+ followed by substrate addition by UV-Vis spectrophotometric analysis	Homo sapiens	14	ALA3414499	single protein format	Scientific Literature
14.	ALA3418004	Binding	Activation of recombinant human ALDH2 using propionaldehyde as substrate at 20 uM preincubated for 2 mins with NAD+ followed by substrate addition by UV-Vis spectrophotometric analysis	Homo sapiens	17	ALA3414499	single protein format	Scientific Literature
15.	ALA3419568	Binding	Effect on recombinant human ALDH2 using propionaldehyde as substrate at 20 uM preincubated for 2 mins with NAD+ followed by substrate addition by UV-Vis spectrophotometric analysis	Homo sapiens	1	ALA3414499	single protein format	Scientific Literature
16.	ALA989246	Binding	Inhibition of human recombinant ALDH2	Homo sapiens	1	ALA1155835	single protein format	Scientific Literature
17.	ALA1019409	Binding	Activation of human ALDH2 assessed as phosphorylated enzyme at 20 uM in absence of rat PKCepsilon by spectrophotometry	Homo sapiens	1	ALA1152868	single protein format	Scientific Literature
18.	ALA1019410	Binding	Activation of human ALDH2 assessed as phosphorylated enzyme at 20 uM in presence of rat PKCepsilon by spectrophotometry	Homo sapiens	1	ALA1152868	single protein format	Scientific Literature
19.	ALA1019419	Binding	Increase in wild type human recombinant ALDH2*1/*1 homotetramer activity expressed in Escherichia coli BL21 cells using wild type allele relative to basal activity	Homo sapiens	1	ALA1152868	cell-based format	Scientific Literature
20.	ALA1019421	Binding	Activation of wild type human recombinant ALDH2*1 expressed Escherichia coli BL21 cells at 10 uM relative to control	Homo sapiens	3	ALA1152868	cell-based format	Scientific Literature