# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA880638 | Binding | Inhibitory activity against decatenation of DNA topoisomerase IV isolated from Escherichia coli | Escherichia coli | 3 | ALA1141057 | single protein format | Scientific Literature | |
2. | ALA983314 | Binding | Induction of Escherichia coli DNA topoisomerase 4-mediated negatively supercoiled Escherichia coli pBR322 DNA cleavage assessed as amount of linear DNA generated per unit amount of enzyme at 50 uM after 10 mins by gel electrophoresis | Escherichia coli | 1 | ALA1154411 | assay format | Scientific Literature | |
3. | ALA983316 | Binding | Induction of Staphylococcus aureus DNA topoisomerase 4 ParC S80L mutant-mediated Escherichia coli pBR322 DNA cleavage assessed as ratio of drug-induced DNA cleavage to spontaneous DNA cleavage at 50 uM after 10 mins by gel electrophoresis | Escherichia coli | 1 | ALA1154411 | assay format | Scientific Literature | |
4. | ALA4374974 | Binding | Inhibition of Escherichia coli DNA topoisomerase 4 subunit ParC assessed as reduction in decatenation of kinetoplast DNA incubated for 60 mins by ethidium bromide staining based gel electrophoresis method | Escherichia coli | 1 | ALA4373693 | single protein format | Scientific Literature | |
5. | ALA4374981 | Binding | Inhibition of Escherichia coli ATCC 25922 DNA topoisomerase 4 subunit ParC assessed as reduction in decatenation of kinetoplast DNA incubated for 30 mins by ethidium bromide staining based gel electrophoresis method | Escherichia coli | 40 | ALA4373693 | single protein format | Scientific Literature |