#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA3428395	Binding	Inhibition of IR (unknown origin) assessed as remaining activity at 100 uM by radioactive filter binding assay in presence of [33P]-ATP relative to control	Homo sapiens	16	ALA3425453	single protein format	Scientific Literature
2.	ALA3428315	Binding	Inhibition of human IR using poly[Glu:Tyr] (4:1) peptide substrate at 1 uM by 33P Hotspot assay	Homo sapiens	1	ALA3425491	single protein format	Scientific Literature
3.	ALA3583130	Binding	Inhibition of Tel-fused INSR (unknown origin) expressed in mouse BA/F3 cells after 48 hrs by luciferase reporter gene assay in absence of recombinant mouse IL3	Homo sapiens	4	ALA3580662	cell-based format	Scientific Literature
4.	ALA3599532	Binding	Inhibition of IR (unknown origin) at 0.5 uM relative to control	Homo sapiens	3	ALA3596148	single protein format	Scientific Literature
5.	ALA3607967	Binding	Inhibition of human IR	Homo sapiens	1	ALA3603755	single protein format	Scientific Literature
6.	ALA3610968	Binding	Inhibition of insulin receptor (unknown origin) by homogeneous time resolved fluorescence assay	Homo sapiens	8	ALA3608257	single protein format	Scientific Literature
7.	ALA3630664	Binding	Inhibition of INSR (unknown origin) assessed as remaining activity at 10 uM by high-throughput assay relative to control	Homo sapiens	1	ALA3627674	single protein format	Scientific Literature
8.	ALA3631257	Binding	Inhibition of IR (unknown origin) at 10 uM after 120 mins P33 radiolabeled kinase activity assay	Homo sapiens	3	ALA3627608	single protein format	Scientific Literature
9.	ALA3707513	Binding	In Vitro Kinase Activity Assay: Compounds to be tested were dissolved in 100% DMSO in a range of concentrations from 10-8 to 10-3 M including negative control (DMSO), then diluted with Kinase Buffer (50 mM HEPES, pH 7.5, 1 mM EGTA, 10 mM MgCl2, 0.01% Tween-20, and 2 mM DTT, added fresh) to 4x the final concentration. The final curve included 11 concentrations (10-11 to 10-5 M) plus negative control DMSO tested in triplicate.Kinase and ATP concentrations necessary for optimal activity were determined in separate experiments. (Table 2). Briefly, increasing concentrations of kinase were incubated in a fixed concentration of ATP (200 uM) in Kinase Buffer to determine maximal activity at various time points (30-120 min). The concentration of kinase that resulted in maximal activity with the shortest incubation time was then incubated in Kinase Buffer with increasing concentrations of ATP.	Homo sapiens	1	ALA3639188	single protein format	BindingDB Database
10.	ALA3734479	Binding	Inhibition of recombinant human INSR using fluorescein-labeled peptide as substrate by fluorescence-electrophoretic mobility shift assay	Homo sapiens	1	ALA3727354	single protein format	Patent Bioactivity Data
11.	ALA3744315	Binding	Inhibition of InsR (unknown origin) at 30 uM by microfluidic assay relative to control	Homo sapiens	19	ALA3739348	single protein format	Scientific Literature
12.	ALA3742721	Binding	Inhibition of INSR (unknown origin)	Homo sapiens	1	ALA3739276	single protein format	Scientific Literature
13.	ALA3751212	Binding	Inhibition of InsR (unknown origin) at 1 uM	Homo sapiens	1	ALA3745631	single protein format	Scientific Literature
14.	ALA3750762	Binding	Inhibition of INSR (unknown origin) at 10 uM	Homo sapiens	9	ALA3745672	single protein format	Scientific Literature
15.	ALA3750796	Binding	Inhibition of INSR (unknown origin) at 1 uM	Homo sapiens	1	ALA3745672	single protein format	Scientific Literature
16.	ALA3751161	Binding	Inhibition of INS1R (unknown origin)	Homo sapiens	1	ALA3745712	single protein format	Scientific Literature
17.	ALA3749383	Binding	Inhibition of interaction of insulin with insulin receptor in human MDA-MB-231 cells assessed as rupture force of binding at 50 uM incubated for 1 hr by atomic force microscopic analysis	Homo sapiens	1	ALA3745706	cell-based format	Scientific Literature
18.	ALA3749384	Binding	Inhibition of interaction of insulin with insulin receptor in human MDA-MB-231 cells assessed as binding probability at 50 uM incubated for 1 hr by atomic force microscopic analysis (Rvb=22.4+/- 2.1%)	Homo sapiens	1	ALA3745706	cell-based format	Scientific Literature
19.	ALA3749385	Binding	Inhibition of interaction of biotinylated insulin with insulin receptor in human MDA-MB-231 cells at 50 uM incubated for 1 hr by avidin-FITC-based flow cytometric analysis	Homo sapiens	1	ALA3745706	cell-based format	Scientific Literature
20.	ALA3749386	Binding	Inhibition of insulin-induced dimerization of GFP-tagged insulin receptor (unknown origin) expressed in human MDA-MB-231 cells assessed as level of monomers at 50 uM by single-molecule fluorescence microscopic analysis (Rvb=59.4%)	Homo sapiens	1	ALA3745706	cell-based format	Scientific Literature