#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA662353	B	In vitro inhibition of supercoiling activity of Escherichia coli DNA gyrase subunit B compared to novabiocin.	Escherichia coli	17	ALA1132006	single protein format	Scientific Literature
2.	ALA1279101	B	Inhibition of Escherichia coli JM109 DNA gyrase subunit B assessed as ATP hydrolysis by ATPase assay	Escherichia coli	33	ALA1275417	single protein format	Scientific Literature
3.	ALA1279102	B	Inhibition of Escherichia coli JM109 DNA gyrase subunit B assessed as DNA triple helix formation by supercoiling assay	Escherichia coli	33	ALA1275417	single protein format	Scientific Literature
4.	ALA1279103	B	Ratio of IC50 for Escherichia coli JM109 DNA gyrase subunit B by ATPase assay to IC50 for Escherichia coli JM109 DNA gyrase subunit B by supercoiling assay	Escherichia coli	33	ALA1275417	single protein format	Scientific Literature
5.	ALA2184195	B	Binding affinity to Escherichia coli GyrB	Escherichia coli	2	ALA2176884	single protein format	Scientific Literature
6.	ALA3367589	B	Inhibition of Escherichia coli GyrB at 50 uM	Escherichia coli	1	ALA3352198	single protein format	Scientific Literature
7.	ALA3405736	B	Inhibition of Escherichia coli GyrB at 100 uM	Escherichia coli	1	ALA3399993	single protein format	Scientific Literature
8.	ALA3630321	B	Inhibition of Escherichia coli Inspiralis DNA gyrase B assessed as reduction in ATPase activity at 100 uM incubated for 100 mins by inorganic phosphate detection assay	Escherichia coli	1	ALA3627613	single protein format	Scientific Literature
9.	ALA3862791	B	Inhibition of Escherichia coli DNA gyrase B ATP binding site assessed as reduction in supercoiling of relaxed pNO1 DNA after 30 mins in presence of biotinylated oligonucleotide by sybrGOLD staining based fluorescence assay	Escherichia coli	22	ALA3861980	single protein format	Scientific Literature
10.	ALA3862795	B	Binding affinity to Escherichia coli DNA gyrase B ATP binding site by surface plasmon resonance assay	Escherichia coli	1	ALA3861980	single protein format	Scientific Literature
11.	ALA3875311	B	Binding affinity to Escherichia coli H560 DNA gyrase B	Escherichia coli	1	ALA3875146	single protein format	Scientific Literature
12.	ALA3875319	B	Inhibition of Escherichia coli H560 DNA gyrase B ATPase activity after 30 mins by ammonium molybdate/malachite green-based phosphate detection assay	Escherichia coli	1	ALA3875146	single protein format	Scientific Literature
13.	ALA4041850	B	Inhibition of Escherichia coli ATCC 25922 DNA gyrase B using relaxed pNO1 DNA as substrate incubated for 30 mins and measured by sybrGOLD staining based fluorescence assay	Escherichia coli ATCC 25922	24	ALA4041481	single protein format	Scientific Literature
14.	ALA4041851	B	Binding affinity to Escherichia coli ATCC 25922 DNA gyrase B by surface plasmon resonance assay	Escherichia coli ATCC 25922	4	ALA4041481	single protein format	Scientific Literature
15.	ALA4041852	B	Inhibition of Escherichia coli DNA ATCC 25922 gyrase B assessed as residual activity at 100 uM using relaxed pNO1 DNA as substrate incubated for 30 mins and measured by sybrGOLD staining based fluorescence assay	Escherichia coli ATCC 25922	34	ALA4041481	single protein format	Scientific Literature
16.	ALA4041855	B	Inhibition of Escherichia coli ATCC 25922 DNA gyrase B using relaxed pNO1 DNA as substrate at 50 uM after 24 hrs by sybrGOLD staining based fluorescence assay	Escherichia coli ATCC 25922	2	ALA4041481	single protein format	Scientific Literature
17.	ALA4155117	B	Inhibition of Escherichia coli C-terminal His6-tagged DNA gyrase B expressed in Escherichia coli BL21(DE3) preincubated for 10 mins followed by ATP addition measured after 30 mins by purine nucleoside phosphorylase enzyme coupled spectrophotometric method	Escherichia coli	16	ALA4152269	assay format	Scientific Literature
18.	ALA4155119	B	Inhibition of Escherichia coli DNA gyrase B	Escherichia coli	1	ALA4152269	single protein format	Scientific Literature
19.	ALA4155124	B	Inhibition of Escherichia coli C-terminal His6-tagged DNA gyrase B expressed in Escherichia coli BL21(DE3) assessed as residual activity at 1 uM preincubated for 10 mins followed by ATP addition measured after 30 mins by purine nucleoside phosphorylase enzyme coupled spectrophotometric method relative to control	Escherichia coli	39	ALA4152269	assay format	Scientific Literature
20.	ALA4198659	B	Inhibition of Escherichia coli DNA gyrase-B at 0.66 to 100 uM using relaxed pUC19 DNA as substrate after 1 hr by bromophenol blue dye based agarose gel electrophoresis method	Escherichia coli	3	ALA4196081	single protein format	Scientific Literature