| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA729047 | B | Percent Enantiomeric composition (R,R) of trans-Dihydrodiol formed by Microsomal Epoxide Hydrolase from rabbit liver microsomes | Oryctolagus cuniculus | 1 | tissue-based format | Scientific Literature | ||
| 2. | ALA729048 | B | Percent Enantiomeric composition (S,S) of trans-Dihydrodiol formed by Microsomal Epoxide Hydrolase from rabbit liver microsomes | Oryctolagus cuniculus | 1 | tissue-based format | Scientific Literature | ||
| 3. | ALA1001507 | B | Inhibition of human microsomal epoxide hydrolase | Homo sapiens | 19 | assay format | Scientific Literature | ||
| 4. | ALA1051705 | B | Inhibition of human mEH | Homo sapiens | 24 | single protein format | Scientific Literature | ||
| 5. | ALA1100146 | B | Inhibition of human microsomal epoxide hydrolase | Homo sapiens | 30 | assay format | Scientific Literature | ||
| 6. | ALA3051825 | B | Inhibition of microsomal epoxide hydrolase (unknown origin) using 7-(2-(Oxiran-2- yl)ethoxy) Resorufin by fluorometric method | Homo sapiens | 32 | assay format | Scientific Literature | ||
| 7. | ALA2393699 | B | Inhibition of human microsomal epoxide hydrolase using PHOME as substrate assessed as formation of 6-methoxy-2-naphthaldehyde incubated for 10 mins prior to substrate addition measured with in 30 mins by fluorescence assay | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 8. | ALA3705305 | B | Inhibition Assay: Inhibition assay of human soluble epoxide hydrolases. | Homo sapiens | 74 | single protein format | BindingDB Database | ||
| 9. | ALA3707738 | B | Activity Assay: The invention also provide methods for assaying for epoxide hydrolase activity as diagnostic assay to identify individuals at increased risk for hypertension and/or those that would benefit from the therapeutic methods of the invention. Any of a number of standard assays for determining epoxide hydrolase activity can be used. For example, suitable assays are described in Gill, et al., Anal Biochem 131, 273-282 (1983); and Borhan, et al., Analytical Biochemistry 231, 188-200 (1995)). Suitable in vitro assays are described in Zeldin et al. J Biol. Chem. 268:6402-6407 (1993). Suitable in vivo assays are described in Zeldin et al. Arch Biochem Biophys 330:87-96 (1996). Assays for epoxide hydrolase using both putative natural substrates and surrogate substrates have been reviewed (see, Hammock, et al. In: Methods in Enzymology, Volume III, Steroids and Isoprenoids, Part B, (Law, J. H. and H. C. Rilling, eds. 1985), Academic Press, Orlando, Fla., pp. 303-311). | Homo sapiens | 145 | organism-based format | BindingDB Database | ||
| 10. | ALA3812612 | B | Inhibition of microsomal epoxide hydrolase in pooled human liver microsomes assessed as enzyme activity using styrene oxide as substrate at 10 uM incubated for 10 mins by UV-HPLC analysis relative to control | Homo sapiens | 23 | tissue-based format | Scientific Literature | ||
| 11. | ALA3825937 | B | Inhibition of recombinant human mEH expressed in baculovirus expression system assessed as formation of 6-methoxy-2-naphthaldehyde preincubated for 5 mins followed by addition of CMNGC as substrate by fluorescent-based assay | Homo sapiens | 4 | assay format | Scientific Literature | ||
| 12. | ALA4187631 | B | Inhibition of microsomal epoxide hydrolase (unknown origin) expressed in baculovirus system using CMNMC substrate by fluorescence based assay | Homo sapiens | 3 | assay format | Scientific Literature | ||
| 13. | ALA4312960 | A | Drug metabolism in human liver microsomes assessed as mEH-mediated oxidoreductive ring-opening of azetidine by measuring [3-[4-[[[3-(hydroxymethyl)oxetan-3-yl]methylamino]methyl]phenoxy]azetidin-1-yl]-[5-(4-methoxyphenyl)-1,3,4-oxadiazol-2-yl]methanone formation by UPLC-Q-TOF/MS analysis | Homo sapiens | 1 | microsome format | Scientific Literature | ||
| 14. | ALA4312961 | A | Drug metabolism in human liver microsomes assessed as mEH-mediated hydrolytic ring-opening of oxetane by measuring [3-[4-[[3,3-bis(hydroxymethyl)azetidin-1-yl]methyl]phenoxy]azetidin-1-yl]-[5-(4-methoxyphenyl)-1,3,4-oxadiazol-2-yl]methanone formation by UPLC-Q-TOF/MS analysis | Homo sapiens | 1 | microsome format | Scientific Literature | ||
| 15. | ALA4312966 | A | Substrate activity at mEH in human liver microsomes assessed as diol release at 10 uM measured after 60 mins in presence of NADPH by UPLC-Q-TOF/MS analysis | Homo sapiens | 33 | microsome format | Scientific Literature | ||
| 16. | ALA4312967 | A | Substrate activity at mEH in human liver microsomes assessed as diol release at 10 uM measured after 60 mins in absence of NADPH by UPLC-Q-TOF/MS analysis | Homo sapiens | 33 | microsome format | Scientific Literature | ||
| 17. | ALA4312968 | A | Substrate activity at mEH in human liver microsomes assessed as amino alcohol release at 10 uM measured after 60 mins in presence of NADPH by UPLC-Q-TOF/MS analysis | Homo sapiens | 19 | microsome format | Scientific Literature | ||
| 18. | ALA4312969 | A | Substrate activity at mEH in human liver microsomes assessed as diol release at 10 uM measured after 60 mins in presence of CYP inhibitor ketoconazole by UPLC-Q-TOF/MS analysis | Homo sapiens | 11 | microsome format | Scientific Literature | ||
| 19. | ALA4312970 | A | Substrate activity at mEH in human liver microsomes assessed as diol release at 10 uM measured after 60 mins in presence of mEH inhibitor-valpromide by UPLC-Q-TOF/MS analysis | Homo sapiens | 10 | microsome format | Scientific Literature | ||
| 20. | ALA4312971 | A | Substrate activity at mEH in human hepatocytes assessed as diol release in presence of mEH inhibitor-valpromide by UPLC-Q-TOF/MS analysis | Homo sapiens | 3 | cell-based format | Scientific Literature |
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