#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA3578998	Binding	Antagonist activity at human mineralocorticoid receptor overexpressed in CHOK1 cells assessed as inhibition of aldosterone-induced protein interaction with coactivator peptide after 6 hrs by luminescence assay	Homo sapiens	32	ALA3576820	cell-based format	Scientific Literature
2.	ALA3579304	Binding	Agonist activity at human mineralocorticoid receptor by cell-based assay	Homo sapiens	34	ALA3576820	cell-based format	Scientific Literature
3.	ALA3579316	Binding	Binding affinity to mineralocorticoid receptor (unknown origin)	Homo sapiens	1	ALA3576820	single protein format	Scientific Literature
4.	ALA3582532	Binding	Antagonist activity at mineralocorticoid receptor (unknown origin) by transcriptional reporter assay	Homo sapiens	1	ALA3580562	single protein format	Scientific Literature
5.	ALA3583484	Binding	Displacement of [3H]-aldosterone from cytosolic fraction of human recombinant mineralocorticoid receptor by scintillation counting analysis	Homo sapiens	17	ALA3580565	single protein format	Scientific Literature
6.	ALA3599033	Binding	Antagonist activity at Gal4 DNA binding domain-tagged human mineralocorticoid receptor ligand binding domain expressed in HEK293 cells assessed as inhibition of receptor-mediated transcriptional activity by luciferase reporter gene assay	Homo sapiens	1	ALA3596154	cell-based format	Scientific Literature
7.	ALA3599055	Binding	Agonist activity at Gal4 DNA binding domain-tagged human mineralocorticoid receptor ligand binding domain expressed in HEK293 cells assessed as activation of receptor-mediated transcriptional activity by luciferase reporter gene assay	Homo sapiens	1	ALA3596154	cell-based format	Scientific Literature
8.	ALA3607224	Binding	Displacement of [3H]-aldosterone from human mineralocorticoid receptor expressed in HEK293 cells by scintillation counting based radioligand competition binding assay	Homo sapiens	18	ALA3603837	cell-based format	Scientific Literature
9.	ALA3705363	Binding	Competitive Molecular Binding Assay: The MR competitive binding assay is based on the binding and displacement of a TAMRA-labeled Dexamethasone probe with fluorescence polarization (FP) detection. The assay is performed in 384-well, low volume NBS black plates (Corning #3676) in assay buffer consisting of 10 mM TES, pH 7.4, 50 mM KCl, 20 mM Sodium molybdate, 1.5 mM EDTA, 0.04% CHAPS, 10% Glycerol and 1 mM DTT. Full Length human mineralocorticoid receptor (hMR) present in a baculovirus infected insect cell lysate is diluted 2 fold in assay buffer and 10 μL of this dilution is added to the assay plate. Blank wells receive 10 μL of the diluted MR lysate containing 3 μM Dexamethasone. 2 μL diluted test compound is transferred to the assay plate for a final starting top concentration of 10 μM in 1% DMSO. The reaction is started by adding 3 μL of 25 nM probe in assay buffer for a final assay concentration of 5 nM.	Homo sapiens	57	ALA3638806	assay format	BindingDB Database
10.	ALA3706043	Binding	Receptor Binding Assay: Using cytosol from progesterone receptor-expressing insect cells (Hi5), competitive binding to the progesterone receptor was determined from the ability to displace 3H-progesterone as reference substance from the receptor. If a compound has an affinity corresponding to progesterone, this corresponds to a competition factor (CF) of 1. CF values greater than 1 are characterized by a lower affinity for the progesterone receptor, and CF values of less than 1 are characterized by higher affinity. The test was carried out as test above, with the following modifications: cytosol from mineralocorticoid receptor-expressing insect cells (Hi5) was used, and the reference substance was 3H-aldosterone.	Homo sapiens	12	ALA3638672	assay format	BindingDB Database
11.	ALA3748816	Binding	Agonist activity at mineralocorticoid receptor (unknown origin) expressed in HEK293 cells assessed as transcriptional activation	Homo sapiens	1	ALA3745677	cell-based format	Scientific Literature
12.	ALA3767967	Binding	Displacement of [3H]-aldosterone from mineralocorticoid receptor (unknown origin) expressed in HEK293 cell lysate incubated overnight by microbeta scintillation counting method	Homo sapiens	5	ALA3763123	cell-based format	Scientific Literature
13.	ALA3776776	Binding	Antagonist activity against 6xHis-MBP-fused human MR LBD domain (732 to 984 amino acid residues) C808S mutant expressed in Escherichia coli BL21(DE3) assessed as inhibition of aldosterone-mediated receptor activation after 16 hrs by luciferase assay	Homo sapiens	26	ALA3774336	assay format	Scientific Literature
14.	ALA3776777	Binding	Displacement of 3H-Cortisol from 6xHis-MBP-fused human MR LBD domain (732 to 984 amino acid residues) C808S mutant expressed in Escherichia coli BL21(DE3) preincubated for 1 hr measured after 30 mins by microbeta plate reader analysis	Homo sapiens	26	ALA3774336	assay format	Scientific Literature
15.	ALA3776778	Binding	Agonist activity against 6xHis-MBP-fused human MR LBD domain (732 to 984 amino acid residues) C808S mutant expressed in Escherichia coli BL21(DE3) assessed as receptor activation after 16 hrs by luciferase assay	Homo sapiens	1	ALA3774336	assay format	Scientific Literature
16.	ALA3817089	Binding	Inhibition of Mineralocorticoid receptor (unknown origin)	Homo sapiens	1	ALA3813625	single protein format	Scientific Literature
17.	ALA653489	Functional	Transcriptional activation activity in CV-1 cells expressing mineralocorticoid receptor	Homo sapiens	1	ALA1129684	cell-based format	Scientific Literature
18.	ALA654292	Functional	Inhibitory activity (IC50) against human mineralocorticoid receptor expressed in CV-1 cells	Homo sapiens	4	ALA1131161	cell-based format	Scientific Literature
19.	ALA654293	Binding	Inhibitory activity (IC50) against human mineralocorticoid receptor expressed in CV-1 cells	Homo sapiens	3	ALA1131161	cell-based format	Scientific Literature
20.	ALA654294	Functional	Inhibition of 10e-9 M aldosterone activity in CV-1 cells expressing mineralocorticoid receptor	Homo sapiens	3	ALA1129684	cell-based format	Scientific Literature