#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA3579960	Binding	Inhibition of human DHFR	Homo sapiens	2	ALA3576868	single protein format	Scientific Literature
2.	ALA3705472	Binding	Inhibition Assay: Antibacterial activity as measured by the minimal inhibitory concentrations (MIC) and minimal bactericidal concentrations of compounds are well known (see., e.g., National Committee for Clinical Laboratory Standards 2000 Performance standards for antimicrobial disk susceptibility tests: approved standard, 7th ed. M2-A7, vol. 20, no. 1, Committee for Clinical Laboratory Standards, Wayne, Pa.).	Homo sapiens	40	ALA3638993	single protein format	BindingDB Database
3.	ALA3707851	Binding	Enzyme Inhibition Assay: Heterocyclic analogs of propargyl-linked inhibitors of the third generation analogs were evaluated in enzyme inhibition assays, assessed for S. aureus and S. pyogenes inhibition, and evaluated for mammalian cell toxicity.	Homo sapiens	15	ALA3639084	single protein format	BindingDB Database
4.	ALA3707564	Binding	Spectrophotometric Enzyme Assay: Compounds were evaluated in spectrophotometric enzyme assays using ChDHFR-TS and hDHFR. Inhibition constants (IC50) were measured (see Table 4). The lead compound, X, has an inhibition constant of 38 nM and modest selectivity (8-fold). All of the biphenyl compounds are more potent than the initial lead compound X and exhibit greater selectivity for the pathogenic enzyme. The most potent racemic compound, D(rac), a 5′-biphenyl derivative, is also the most selective of the racemic compounds (944-fold). The single R enantiomer of this 5′-biphenyl analog is the most potent (1.1 nM) and most selective (1273-fold) of all known compounds tested against the Cryptosporidium DHFR enzyme.	Homo sapiens	11	ALA3639084	single protein format	BindingDB Database
5.	ALA3738282	Binding	Competitive inhibition of human recombinant DHFR preincubated for 2 mins followed by substrate addition in presence of dihydrofolate	Homo sapiens	2	ALA3734661	single protein format	Scientific Literature
6.	ALA3750438	Binding	Inhibition of human recombinant DHFR expressed in Escherichia coli by spectrophotometric analysis	Homo sapiens	8	ALA3745645	assay format	Scientific Literature
7.	ALA3755622	Binding	Inhibition of human DHFR using 45 uM DHF as substrate by spectrophotometry	Homo sapiens	2	ALA3751663	single protein format	Scientific Literature
8.	ALA3788092	Binding	Inhibition of human recombinant dihydrofolate reductase using dihydrofolate as substrate measured every 30 secs over 6 mins by UV/visible spectrophotometer in presence of NADPH	Homo sapiens	8	ALA3784942	single protein format	Scientific Literature
9.	ALA3789540	Binding	Inhibition of human DHFR assessed as conversion of dihydrofolic acid to tetrahydrfolic acid by enzyme immunoassay in presence of NADPH	Homo sapiens	10	ALA3785084	single protein format	Scientific Literature
10.	ALA3789541	Binding	Binding affinity to human DHFR by UV-Vis spectral analysis	Homo sapiens	2	ALA3785084	single protein format	Scientific Literature
11.	ALA3789542	Binding	Competitive inhibition of human DHFR at 20 to 30 uM preincubated with NADPH followed by dihydrofolic acid substrate addition by Lineweaver-Burk plot	Homo sapiens	1	ALA3785084	single protein format	Scientific Literature
12.	ALA3829095	Binding	Inhibition of recombinant human DHFR assessed as reduction in NADPH oxidation using dihydrofolate as substrate preincubated for 5 mins followed by substrate addition	Homo sapiens	9	ALA3826924	single protein format	Scientific Literature
13.	ALA3829123	Binding	Inhibition of recombinant human DHFR assessed as reduction in NADPH oxidation using dihydrofolate as substrate by fluorescence spectrophotometric analysis	Homo sapiens	1	ALA3826924	single protein format	Scientific Literature
14.	ALA651328	Binding	Tested for the inhibition of [14C]-DDATHF influx in CCRF-CEM cells of human leukemic lymphoblast		1	ALA1127804	cell-based format	Scientific Literature
15.	ALA666224	Binding	Concentration required for the inhibition of dihydrofolate reductase		1	ALA1145818	single protein format	Scientific Literature
16.	ALA666225	Binding	Inhibitory activity against Dihydrofolate reductase		1	ALA1133617	single protein format	Scientific Literature
17.	ALA666226	Binding	Competitive inhibition of mammalian Dihydrofolate reductase		1	ALA1122041	single protein format	Scientific Literature
18.	ALA666227	Binding	Competitive inhibition of mammalian dihydrofolate reductase		1	ALA1122041	single protein format	Scientific Literature
19.	ALA666228	Binding	Effect against dihydrofolate reductase (DHFR)		1	ALA1124413	single protein format	Scientific Literature
20.	ALA666229	Binding	Binding affinity against Dihydrofolate reductase		1	ALA1135481	single protein format	Scientific Literature