Antagonist activity at Gal4 DNA binding domain-tagged human VDR expressed in HEK293 cells assessed as inhibition of receptor-mediated transcriptional activity by luciferase reporter gene assay
Agonist activity at Gal4 DNA binding domain-tagged human VDR expressed in in HEK293 cells assessed as activation of receptor-mediated transcriptional activity by luciferase reporter gene assay
Agonist activity at human VDR transfected in African green monkey COS7 cells assessed as induction of transcriptional activity at 10'-10 to 10'-5 M after 16 hrs by dual luciferase reporter gene assay
Antagonist activity at human VDR transfected in African green monkey COS7 cells assessed as inhibition of 1alpha,25-dihydroxyvitamin D3-induced transcriptional activity after 16 hrs by dual luciferase reporter gene assay
Antagonist activity at human VDR expressed in HEK293 cells co-expressing CMX-GAL4N and TK-MH100x4-LUC assessed as inhibition of 1alpha,25(OH)2VD3-induced transcriptional activity up to 10 uM by beta-galactosidase reporter gene assay
Agonist activity at human VDR expressed in HEK293 cells co-expressing CMX-GAL4N and TK-MH100x4-LUC assessed as induction of transcriptional activity by beta-galactosidase reporter gene assay
Agonist activity at human VDR expressed in HEK293 cells co-expressing CMX-GAL4N and TK-MH100x4-LUC assessed as induction of transcriptional activity at 10 uM by beta-galactosidase reporter gene assay relative to 1alpha,25(OH)2VD3
Agonist activity at human VDR expressed in HEK293 cells co-expressing CMX-GAL4N and TK-MH100x4-LUC assessed as induction of transcriptional activity at 3 uM by beta-galactosidase reporter gene assay relative to 1alpha,25(OH)2VD3
Partial agonist activity at VDR in human HEK293 cells assessed as transcriptional activity after 16 to 24 hrs by luciferase reporter gene assay relative to 1,25(OH)2D3