| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA864097 | F | Agonist activity at TSHR expressed in HEK293 EM cells measured by intracellular cAMP accumulation | Homo sapiens | 15 | cell-based format | Scientific Literature | ||
| 2. | ALA864098 | F | Agonist activity at TSHR expressed in HEK293 EM cells measured by intracellular cAMP accumulation relative to TSH | Homo sapiens | 15 | cell-based format | Scientific Literature | ||
| 3. | ALA1964011 | F | PUBCHEM_BIOASSAY: qHTS for antagonists of the Thyroid Stimulation Hormone Receptor: Hit Validation in Primary Screen. (Class of assay: confirmatory) | 844 | assay format | PubChem BioAssays | |||
| 4. | ALA1794527 | F | PUBCHEM_BIOASSAY: Antagonists of the Thyroid Stimulating Hormone Receptor: Validation. (Class of assay: confirmatory) [Related pubchem assays (depositor defined):AID504813] | 5 | assay format | PubChem BioAssays | |||
| 5. | ALA1963987 | F | PUBCHEM_BIOASSAY: qHTS for antagonists of the Thyroid Stimulation Hormone Receptor: Hit Validation in Parental Cell Line. (Class of assay: confirmatory) | 127 | assay format | PubChem BioAssays | |||
| 6. | ALA1614281 | F | PUBCHEM_BIOASSAY: qHTS Assay for Agonists of the Thyroid Stimulating Hormone Receptor: Activators of Intracellular cAMP Concentrations in Parental HEK 293. (Class of assay: confirmatory) | Homo sapiens | 12,194 | assay format | PubChem BioAssays | ||
| 7. | ALA1614014 | F | PUBCHEM_BIOASSAY: Confirmation Concentration-Response Assay for Agonists of the Thyroid Stimulating Hormone Receptor. (Class of assay: confirmatory) | Homo sapiens | 86 | assay format | PubChem BioAssays | ||
| 8. | ALA1614361 | F | PUBCHEM_BIOASSAY: qHTS Assay for Agonists of the Thyroid Stimulating Hormone Receptor. (Class of assay: confirmatory) | Homo sapiens | 16,181 | assay format | PubChem BioAssays | ||
| 9. | ALA1614489 | F | PUBCHEM_BIOASSAY: Secondary Concentration-Response Assay for Agonists of the Thyroid Stimulating Hormone Receptor: HTRF Activity Detection. (Class of assay: confirmatory) | Homo sapiens | 90 | assay format | PubChem BioAssays | ||
| 10. | ALA1613852 | F | PUBCHEM_BIOASSAY: Confirmation Concentration-Response Assay for Agonists of the Thyroid Stimulating Hormone Receptor: Activators of Intracellular cAMP Concentrations in Parental HEK 293. (Class of assay: confirmatory) | Homo sapiens | 11 | assay format | PubChem BioAssays | ||
| 11. | ALA1738625 | F | PUBCHEM_BIOASSAY: qHTS Assay for Antagonists of the Thyroid Stimulating Hormone Receptor. (Class of assay: confirmatory) [Related pubchem assays (depositor defined):AID488990] | Homo sapiens | 5 | assay format | PubChem BioAssays | ||
| 12. | ALA1614326 | F | PUBCHEM_BIOASSAY: Secondary Concentration-Response Assay for Agonists of the Thyroid Stimulating Hormone Receptor: ELISA Activity Detection. (Class of assay: confirmatory) [Related pubchem assays (depositor defined):AID1401, AID1402, AID1403, AID926, AID933, AID939] | Homo sapiens | 32 | assay format | PubChem BioAssays | ||
| 13. | ALA5256331 | B | Antagonist activity at human TSHR expressed in HEK293 cells assessed as reduction in cAMP production incubated for 2 hrs by Eu-cAMP tracer based TR-FRET assay | Homo sapiens | 23 | cell-based format | Scientific Literature | ||
| 14. | ALA5256332 | B | Antagonist activity at human TSHR expressed in HEK293 cells assessed as reduction in cAMP production by measuring its efficacy at 10 uM incubated for 2 hrs by Eu-cAMP tracer based TR-FRET assay | Homo sapiens | 23 | cell-based format | Scientific Literature | ||
| 15. | ALA5256341 | B | Antagonist activity at human TSHR in HEK293 cells assessed as inhibition of bTSH-induced cAMP production incubated for 2 hrs by Eu-cAMP tracer based TR-FRET assay | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 16. | ALA5256362 | B | Allosteric antagonist activity at human TSHR expressed in CHO cells assessed as inhibition of bTSH-induced cAMP production incubated for 2 hrs by luminescence assay | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 17. | ALA3227399 | B | Agonist activity at TSHR (unknown origin) | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 18. | ALA3227400 | F | Agonist activity at TSHR (unknown origin) transiently transfected HEK-EM293 cells assessed as cAMP level after 1 hr by ELISA | Homo sapiens | 24 | cell-based format | Scientific Literature | ||
| 19. | ALA3227401 | F | Agonist activity at TSHR (unknown origin) transiently transfected HEK-EM293 cells assessed as cAMP level after 1 hr by ELISA relative to N-(4-(5-(3-Benzyl-5-hydroxy-4-oxo-1,2,3,4-tetrahydroquinazolin-2-yl)-2-methoxybenzyloxy)phenyl)acetamide | Homo sapiens | 23 | cell-based format | Scientific Literature | ||
| 20. | ALA3887131 | F | Antagonist Activity Assay: Antagonist activity of three test compounds against human TSI at the human TSH receptor was tested in Chinese Hamster Ovary (CHO) cells (cultured 16 h in the absence of serum prior to the assay) stably expressing the human TSH receptor and a CRE-driven firefly luciferase reporter gene. TSI was partially purified from the serum of a GD patient by filtration over a 0.45 mm filter, protein G-Sepharose column chromatography, dialysis against phosphate-buffered saline and subsequent concentrating on a 10K Amicon filter. It was confirmed that the TSI preparation does not activate luciferase activity in control CHO cells lacking the human TSHR. The cAMP phosphodiesterase inhibitor rolipram was included in the assay medium (10 μM) to augment TSHR-induced CRE-luciferase synthesis, which was quantified using a luminescence counter. The cells were incubated with compound A, B or C (0.316 nM-10 μM) together with 3.16 mg/ml TSI (or bovine TSH at a equieffective concentration of 18 nM). | 24 | cell-based format | BindingDB Database |
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