# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA3431457 | Functional | GSK_TCAKS: pIC50 NIH 3T3 cell line viability assay using luminiscence. Tissue culture surface treated assay plates (1536-well) were used. 5uL of a 2 * 10^5 cells per mL solution were dispensed per well (~10^3 host cells), into plates already containing compounds which had been pre-dispensed. The 100% cells survival control was determined exposing the host-cells to a 0.5% DMSO concentration, whereas the 100% toxicity control was achieved by seeding the cells in presence of Amphotericin B at 50uM. Plates were incubated for four days at 37C, 5% CO2. The assay readout was developed by addition of 5uL of CellTiter-Glo Reagent following the manufacturer's instructions. Luminiscence was measured using a ViewLux. | Mus musculus | 222 | ALA3430912 | cell-based format | GSK Kinetoplastid Screening | |
2. | ALA615595 | Functional | Effective dose required against Trypanosoma cruzi amastigotes in 3T3 Fibroblasts | Mus musculus | 3 | ALA1133799 | cell-based format | Scientific Literature | |
3. | ALA615596 | Functional | Effective dose required against Trypanosoma cruzi amastigotes in 3T3 Fibroblasts | Mus musculus | 1 | ALA1133799 | cell-based format | Scientific Literature | |
4. | ALA615598 | Functional | Ability of the peptide to inhibit 6 nM bombesin induced [3H]thymidine incorporation was measured in swiss 3T3 cells using mitogenicity assay; na=Not active | Mus musculus | 1 | ALA1127325 | cell-based format | Scientific Literature | |
5. | ALA615599 | Functional | Ability of the peptide to inhibit 6 nM bombesin induced [3H]thymidine incorporation was measured in swiss 3T3 cells using mitogenicity assay; nt=Not tested | Mus musculus | 1 | ALA1127325 | cell-based format | Scientific Literature | |
6. | ALA615600 | Functional | Effective dose against murine 3T3 fibroblasts cells | Mus musculus | 40 | ALA1148384 | cell-based format | Scientific Literature | |
7. | ALA615601 | Functional | Dose required against murine 3T3 fibroblasts cells; 1-10 uM | Mus musculus | 4 | ALA1148384 | cell-based format | Scientific Literature | |
8. | ALA615602 | Functional | Cytotoxic effect on 3T3 cells | Mus musculus | 1 | ALA1127530 | cell-based format | Scientific Literature | |
9. | ALA615603 | Functional | Cytotoxic effect on 3T3 cells | Mus musculus | 13 | ALA1127530 | cell-based format | Scientific Literature | |
10. | ALA615604 | Functional | Compound was tested for the concentration necessary to inhibit swiss 3T3 Mouse Fibroblast cell growth rate by 50%. | Mus musculus | 5 | ALA1127622 | cell-based format | Scientific Literature | |
11. | ALA615605 | Functional | Concentration of NO in supernatant generated by mouse fibroblasts (3T3) cell line in the presence of compound was determined using NR coloration | Mus musculus | 1 | ALA1149233 | cell-based format | Scientific Literature | |
12. | ALA615606 | Functional | Concentration of NO in supernatant generated by mouse fibroblasts (3T3) cell line in the presence of compound was determined using NR coloration; nd=No data | Mus musculus | 1 | ALA1149233 | cell-based format | Scientific Literature | |
13. | ALA884526 | Functional | In vitro cytotoxic concentration against mouse fibroblasts (3T3) cell line using MTT coloration | Mus musculus | 13 | ALA1149233 | cell-based format | Scientific Literature | |
14. | ALA615607 | Functional | In vitro cytotoxic concentration against mouse fibroblasts (3T3) cell line using MTT coloration; n.d.=no data | Mus musculus | 7 | ALA1149233 | cell-based format | Scientific Literature | |
15. | ALA615608 | Functional | In vitro cytotoxic concentration against mouse fibroblasts (3T3) cell line using MTT coloration; n.t.=not tested | Mus musculus | 1 | ALA1149233 | cell-based format | Scientific Literature | |
16. | ALA615609 | Functional | In vitro inhibitory activity against mouse fibroblasts (3T3) cell line using CV coloration | Mus musculus | 13 | ALA1149233 | cell-based format | Scientific Literature | |
17. | ALA615682 | Functional | In vitro inhibitory activity against mouse fibroblasts (3T3) cell line using CV coloration; n.d.=no data | Mus musculus | 7 | ALA1149233 | cell-based format | Scientific Literature | |
18. | ALA615683 | Functional | In vitro inhibitory activity against mouse fibroblasts (3T3) cell line using CV coloration; n.t.=not tested | Mus musculus | 1 | ALA1149233 | cell-based format | Scientific Literature | |
19. | ALA615684 | Functional | In vivo inhibition of H-ras-transformed 3T3 fibroblasts in nude mouse tumor models | Mus musculus | 1 | ALA1149326 | organism-based format | Scientific Literature | |
20. | ALA615686 | Functional | Inhibition of Swiss 3T3 Mouse fibroblast proliferation | Mus musculus | 10 | ALA1127560 | cell-based format | Scientific Literature |