# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA1012282 | B | Binding affinity to human Cyclophilin B | Homo sapiens | 1 | ALA1155051 | single protein format | Scientific Literature | |
2. | ALA1273324 | B | Binding affinity to cyclophilin B by ELISA | 20 | ALA1269000 | single protein format | Scientific Literature | ||
3. | ALA2428072 | B | Inhibition of CypB PPIase activity (unknown origin) | Homo sapiens | 1 | ALA2424541 | single protein format | Scientific Literature | |
4. | ALA3389005 | B | Binding affinity to human cyclophilin B by surface plasmon resonance method | Homo sapiens | 4 | ALA3351593 | single protein format | Scientific Literature | |
5. | ALA3705673 | B | Enzymatic Assay: Cyclophilin PPlase activity was measured at 20 C. by using the standard chymotrypsin coupled assay (Kofron J L, Kuzmic P, Kishore V, Colon-Bonilla E, Rich D H. Determination of kinetic constants for peptidyl prolyl cis-trans isomerases by an improved spectrophotometric assay. Biochemistry. 1991 Jun. 25; 30(25):6127-34). The assay buffer (25 mM Hepes, 100 mM NaCl, pH 7.8) and CypA, B or D (1900 nM stock solution) were pre-cooled to 4 C., to which then was added 5 uL of 50 mg/ml chymotrypsin in 1 mM HCl. The reaction was initiated by adding 20 uL of 3.2 mM peptide substrate (Suc-Ala-Ala-cis-Pro-Phe-pNA) in LiCl/TFE solution with rapid inversion. After a delay from the onset of mixing, the absorbance of p-nitroaniline was followed at 390 nM until the reaction was complete (1 min). The final concentration of LiCl in the assay was 20 mM; TFE was present at a concentration of 4% (v/v). Absorbance readings were collected every 1 s by spectrophotometer. | Homo sapiens | 11 | ALA3639117 | single protein format | BindingDB Database | |
6. | ALA3756832 | B | Binding affinity to Cyclophilin B (unknown origin) at 10 to 30 uM by surface plasmon resonance analysis | Homo sapiens | 1 | ALA3751796 | single protein format | Scientific Literature | |
7. | ALA3756834 | B | Binding affinity to Cyclophilin B (unknown origin) by surface plasmon resonance analysis | Homo sapiens | 2 | ALA3751796 | single protein format | Scientific Literature | |
8. | ALA4668628 | B | Inhibition of C-terminal His6-tagged CypB (unknown origin) expressed in Escherichia coli C41(DE3) using Suc-Ala-Ala-Cis-Pro-Phe-pNA as substrate by spectrophotometric analysis | Homo sapiens | 4 | ALA4665682 | assay format | Scientific Literature | |
9. | ALA4712040 | B | Inhibition of PPIB (unknown origin) pre-incubated for 20 mins followed by fluorescence substrate addition and measured after 120 mins by DiFMUP assay | Homo sapiens | 1 | ALA4706627 | single protein format | Scientific Literature | |
10. | ALA5253385 | B | Inhibition of CypB (unknown origin) | Homo sapiens | 2 | ALA5252525 | single protein format | Scientific Literature | |
11. | ALA5253389 | B | Binding affinity to CypB (unknown origin) | Homo sapiens | 2 | ALA5252525 | single protein format | Scientific Literature |