mAb (6G8-F4-E3); Mouse anti Human DDX3 Antibody; WB, ICC, IF, IP; Unconjugated
Basic Description
Product Name
DDX3 Mouse mAb - Primary antibody, specific to DDX3, Mouse IgG2a
Synonyms
ATP dependent RNA helicase DDX3X antibody; ATP-dependent RNA helicase DDX3X antibody; CAP Rf antibody; DBX antibody; DDX14 antibody; DDX3X antibody; DDX3X_HUMAN antibody; DEAD (Asp Glu Ala Asp) box polypeptide 3 X linked antibody; DEAD (Asp-Glu-Ala-Asp) b
Specifications & Purity
ExactAb™, Validated, 1.0 mg/mL
Host species
Mouse
Specificity
DDX3
Immunogen
A synthetic peptide derived from human DDX3 (AA 22-178)
Positive Control
WB: Jurkat, K562, HeLa, A431, HEK293, NIH/3T3 and C6 cell lysates. ICC/IF: HeLa cells. IP: HeLa lysates.
Conjugation
Unconjugated
Grade
ExactAb™, Validated
Product Description
Mouse anti Human DDX3 Antibody, Monoclonal (6G8-F4-E3), could be used for WB, ICC, IF, IP and so on. Application WB: 1/500-1/1000 ICC/IF: 1/50-1/200 IP: 1/20 Protein Function ATP-dependent RNA helicase. Acts as a cofactor for XPO1-mediated nuclear export of incompletely spliced HIV-1 Rev RNAs. Also involved in HIV-1 replication. Interacts specifically with hepatitis C virus core protein resulting in a change in intracellular location.
Product Properties
Isotype
Mouse IgG2a
Light Chain Type
kappa
SDS-PAGE
150 kDa
Purification Method
Protein G purified
Form
Liquid
Concentration
1.0 mg/mL
Storage Temp
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
Images
DDX3 Mouse mAb (Ab099443) - Western Blot All lanes: DDX3 Mouse mAb (Ab099443) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 73 kDa Observed band size: 75 kDa Exposure time: 18.7 s
DDX3 Mouse mAb (Ab099443) - IF/ICC Immunocytochemistry analysis of DDX3 (6G8) in HeLa using DDX3 Mouse mAb (Ab099443).
DDX3 Mouse mAb (Ab099443) - IP Immunoprecipitation analysis of DDX3 in HeLa lysates using DDX3 Mouse mAb (Ab099443).