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DDX3 Mouse mAb - Primary antibody, specific to DDX3, Mouse IgG2a

  • ExactAb™
  • Validated
  • 1.0 mg/mL
Features and benefits
  • Short Overview:

    mAb (6G8-F4-E3); Mouse anti Human DDX3 Antibody; WB, ICC, IF, IP; Unconjugated

  • Species reactivity(Reacts with): Human,Monkey,Mouse,Rat
  • Isotype: Mouse IgG2a
    Application:
  • ICC/IF
  • IP
  • WB
Item Number
Ab099443
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab099443-10μl
10μl
In stock
$69.90
Ab099443-50μl
50μl
In stock
$199.90
Ab099443-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$309.90
Ab099443-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,499.90

mAb (6G8-F4-E3); Mouse anti Human DDX3 Antibody; WB, ICC, IF, IP; Unconjugated

Basic Description

Product NameDDX3 Mouse mAb - Primary antibody, specific to DDX3, Mouse IgG2a
SynonymsATP dependent RNA helicase DDX3X antibody; ATP-dependent RNA helicase DDX3X antibody; CAP Rf antibody; DBX antibody; DDX14 antibody; DDX3X antibody; DDX3X_HUMAN antibody; DEAD (Asp Glu Ala Asp) box polypeptide 3 X linked antibody; DEAD (Asp-Glu-Ala-Asp) b
Specifications & PurityExactAb™, Validated, 1.0 mg/mL
Host speciesMouse
SpecificityDDX3
ImmunogenA synthetic peptide derived from human DDX3 (AA 22-178)
Positive ControlWB: Jurkat, K562, HeLa, A431, HEK293, NIH/3T3 and C6 cell lysates. ICC/IF: HeLa cells. IP: HeLa lysates.
ConjugationUnconjugated
GradeExactAb™, Validated
Product Description

Mouse anti Human DDX3 Antibody, Monoclonal (6G8-F4-E3), could be used for WB, ICC, IF, IP and so on.
Application
WB: 1/500-1/1000
ICC/IF: 1/50-1/200
IP: 1/20
Protein Function
ATP-dependent RNA helicase. Acts as a cofactor for XPO1-mediated nuclear export of incompletely spliced HIV-1 Rev RNAs. Also involved in HIV-1 replication. Interacts specifically with hepatitis C virus core protein resulting in a change in intracellular location.

Product Properties

IsotypeMouse IgG2a
Light Chain Typekappa
SDS-PAGE150 kDa
Purification MethodProtein G purified
FormLiquid
Concentration1.0 mg/mL
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageStore at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

DDX3 Mouse mAb (Ab099443) - Western Blot
All lanes: DDX3 Mouse mAb (Ab099443) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution

Predicted band size: 73 kDa
Observed band size: 75 kDa
Exposure time: 18.7 s

DDX3 Mouse mAb (Ab099443) - IF/ICC
Immunocytochemistry analysis of DDX3 (6G8) in HeLa using DDX3 Mouse mAb (Ab099443).

DDX3 Mouse mAb (Ab099443) - IP
Immunoprecipitation analysis of DDX3 in HeLa lysates using DDX3 Mouse mAb (Ab099443).

Associated Targets

DDX3X Tchem ATP-dependent RNA helicase DDX3X 0 Activities

Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)

Application

ApplicationDilution info
WB

1/500-1/1000

ICC/IF

1/50-1/200

IP

1/20

Certificates

Certificate of Analysis(COA)

Enter Lot Number to search for COA:

To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section

2 results found

Lot NumberCertificate TypeDateItem
ZJ24F0505897Certificate of AnalysisMay 21, 2024 Ab099443
ZJ24F0505896Certificate of AnalysisMay 21, 2024 Ab099443

Related Documents

Solution Calculators